CR01873

CRIMIC CR01873

Gene CG8560

CG8560

Information

Donor Location Cytology FBti Stock number
pM37-p23L:7402332..740418165F11Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK TTTCACAACGTGGCTTAGAGGGAGGA AGCGTACAGAACGTTGGTGGAACT

Images

Construct Design Sequences

sgRNA sequence TGTGCAAGTTACAGACTAAATGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GGCACTGCTGATTGGCTTTC TGCACAAAACCTCTTAGCCAG TAATTGCCCGTGGAACCACT AGCAGCAGCCGAAAGTTTTG
 
Amplicon 5' Amplicon 3'
GGCACTGCTGATTGGCTTTCTGGTCTTGCGCCACATACGGGTGGTGGTGTGAGAGTATTCATAGCCATCGGGATTCACCACCGGCAGGATAACCCAGTCAAAGTCCTTCAGCAGTTCCGAGTTGGCAGCAAAGTTCTCCACCAGCTGATGGATCACATACAGAGCTCCAGCATGGGCGATCCATTCGCGAGCATGGATACCAGCATCCAGGAAGACAACATTCTTGCCGGTCTTACCATCGCCATTGGTGATAGTGATGGTCTTGATGTCACGATTCTCATACGACTTGCCAGCCACCTGGACGGATACACGGCTGGGATAGGCAGCAGCCAGTTCATCCAGGTAGGCATTGATCTCGGCATGACGATGGAACGCCTTGAAGCTAACGCTGCGTTCAGCGGATCGCAGGTTCATCAACCTCTGGTTCTGGTTCTCATCGCGCTCCTGACGCAGCGACTCACCGAAATTCTCGTTGATCACCGAGTAGTTTACGCCGTACTTCTCCAGCAGGTGCTCGAATCCCTCCTGATCCTCGGGCTTGACCATCACGCGACTGGACGCGTCCAGGGAACGGGGCAGGTCAAAGAAGTCGTATGCCTCATTTCCGGACAAACGCAGCAGCAGCTGCTTCTCAAAAGCGTTGCGAGCATTGATGTCGTAGATCTTGTAGCTGTAGATAAAAAAAAATCCATTTTAAGTTGCTTCGAACTTATTTTTTTTTTTAGAGTTTCACGAGCTTTAAAAGTTGATATTTTTAAAATATCCTATTAAAATCAGCTTGCACCCCTTGATTTAATTGAATCACGCTGGCTAAGAGGTTTTGTGCA TAATTGCCCGTGGAACCACTCCTCCTCCGCCGGCTCACTCACCCATCGTAGTTTTCCACCGCCAAGGCCACGCTGGCCAGCAGGAGCACCACAGCAAATAGTTTCAACATTTTTCAGTCCGCTCCGTTCCGTTTGGGCCCAGGGCTTCTAATGTGGCTCGAATACACAGATTGTGGCTTATATAGCCAACTGCCAGCTCAGCGATCATTCACAGCCCACCACCCCTTTTGGGCTTCTTATCAATGAAGAACTCTATCGGTAAAGAGCAGTTATCAGTGCGCAGATGATGGGCGATGTTCGATTTCATTTGATTTGATTTGTTGTTGTGTCTTCGCCGAGCGATGGAGAAATTCTAAAAAAAAATCTCTCAAAATTTATACTCATTGACAAGAGTATGACAGACTGGCTCGTAGTAAAAGTTTTTCACTTGACTAACTCATGACTACTCGGCAGGGTTTTAAAACATTTTGTTTTGTTACGTTAATGGCTGGAAAATAAGTTGAGTCAATGAATAAAACGCATTTAAAGTAAACCGTTTTGATTGTGAATTGCAAATTTTTAAATAAACAACTATGTGCTTGGGTCAATTTTGTATATGTCATTCACCGAAGATATCTGACATTTTTAGACGGCTTTGGAGCGACTCGTAAATTTGGTCTTCTGACTTTGCGCAATCAACATTTGATTTATATGGCCTTTTCGGCCTTAGACTCCGTCAATTCTTGGAGTGCGCCAGTCGCAGTCTCTATCTTTATTTTTTTTTTGGTATTTGAATTTGTTTGCAAAACGTGGCTTTATCAGTGTGCTCTGTGCACTGCACCAAGAAAAAAGTTTTCGGTTTAAAACAATGAAAGTCCGTACAAAAACAAAAAGAAAAACTAACGCAAAAACAAAAAAATCAAAAACAACTAACTAAAAACTCCGGCTGCCGCGCACACAAAATATATTTGAAAATTTATTGCATTTCATGAGTTTTTCCATATTTGTCTGCATTTGTAGGCAGAGTTTTGGTCACAAAACTTTCGGCTGCTGCT
 

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