CR01868

CRIMIC CR01868

Gene CG13950

CG13950

Information

Donor Location Cytology FBti Stock number
pM37-p22L:..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK short band

Images

Construct Design Sequences

sgRNA sequence GACTTTAACCCAGATCAAAGCGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
AGGCCAAGATGGAGAACACG AGTGGAGAACCGGGTATCCA TCTGGGTTAAAGTCAAAGGTCA TCCGATCGAAGTTCACGTGG
 
Amplicon 5' Amplicon 3'
AGGCCAAGATGGAGAACACGGCTAGAAGACATTCTAGTAATAGTGGTTACTAGGAGTACTAAAATGTAGCAGTAAGATATAAAAAGGATATGAGCCACCATCAAGGAGTGCCTATTCCTCCAACTAGCACTTATACTTTCTTAATATGAATTTAAGATGACAATTACAACATATTCCTTGTATTTTTATAACTAGTAGCTTGCAAAGTTATTCGGGCAATTAATTTTATGATCGGAAATTCATTATGCGATAAGCAGTTTTTCGAGTTAGGATCATAAATGTTTTATCTTCGAAAAAGTATTACAACGCTTCCGGGTTTCCCAAGTTATGTGGTGCAACAATACTGAATATTCAATATTTAAGCAAAAATAAGGATAATAACTTATCTTTGGCTAGTTTTTCTCCCAGTGTCGCTGGTAACTAGCAACAAATGGGCAGTCGGCAGTTGGGGGCGTGACAGCAACCGTGAAAAGAACTCGAGGATGACAGTTATGGGTTACAAAGGCAAGGATTGAATCAAATAAATAAACGCGCAAGGTTCTCGTCATGGAGCGCGGCTCACTGGGTCGGTTATTAGGTGGCCAGGGGGACAGGGAGCCACCACCGAGTGGATTTATATGGGATTCGATTGAACGGGTTGAGCTCCGACCGGGGTGGGTTCGGGCATGGGCCTGGGGGTAACCTTACCACGTCCACATCCACGTCCATGTCAAGTTTGCATGCGGCTGCAGGTAATTCCCCATTTCACCCGCCTTTACATAATCACAACAAATGAACGAGGAAAGGCGACGGATGGCGAATCTATTAGCATAAGGGCGCATAAAACGGCGCCGGATCCGGCTGGATTCCATCGAGGATTCGCACTCGTCGACTCAGTTGCAGTGGAGCCGTGGAGCCGCGATGAACGTTTGGAAAGCAAAGGTCCTAACGGAGGTCCCATTCGGACACGTCCTCATCGTCAGTGGGCGCGTCAAGCCTCATCCGAATAAGTAATCATCTTTCATAGTTTGGATACCCGGTTCTCCACT TCTGGGTTAAAGTCAAAGGTCAAATGTAGAAGCTGATTTTCTAACCAAATGAAATTAACCAAATGAAATAAACCAATTAGTTCAAAAAGTAAAAAGAGATTAAGTATAAATGGTATTCGAATTATCCGATTATTGACAAAACAATGCAGTTCTAATGCCAACGAAAGGCTTATAAAGCTGTGACAGAGATTTGCAGAAATGAAATGGGTATCCTTAGCGAGTTTGGGGGAGTGCCGAGTATCCTCCCAATCCATATCTAATACTTTTTACTAATGACACCTCTCGAATTACGGACAGAATTTCGCTGGACCTCACGGACAATGTGAACGCGCAGAACGAGAGCGAAACGGTGTTCCTGAAGATCGAGGCCAATTTCCGCGAGGGCCAAATCATCCGCAGCATGTTCCAGCCGGGCGGGGGATGGCAGCAGGAGGAGATCTCGAAGAACTGGAAGTGCGACGGCCCAAAGAACCCGCTGCAGCCTGGCCAGAGCTTCACCTTCCGGGTGGCGGTGCTGCAGCGATGCTTCGAGATCTACGTGAACGACCAGCTGTACGGGTCCTTCGAGTTCGTCAAGTTCCCCAAGCAAATCAACTACGTGCGCACCTACGGCGACTTCGAGAAGATCACCCAGTTCCACCACCGCATGCTCTTTCCGCTCGTCTTCCCCAGAACGCTCATGTGTCCGGACAAGGTGGCCTTCCAGAGCGACGTGCCCAGGCGGTATGAAACCGGCACAGTGGTGGCCATGGAGTGCATCGCCAAGGGCCCGCCGACCACGGAGTTCTCCATCTGCTTCCAGTGCAACGACACCGGGCGTACGGTGCTCCGGTTCCACGTGAACTTCGATCGGA
 

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