CR01856

CRIMIC CR01856

Gene CG17029

CG17029

Information

Donor Location Cytology FBti Stock number
pM37-p03L:..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
long band OK

Images

Construct Design Sequences

sgRNA sequence ATGACATTATTCCTGCAAACAGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
TCCTCTGCACATGAACTGCT CAGGAATAATGTCATGCACGGT CAGGTTTATCGGTGCACAGC CGTACACGGTGACCAAGTCA
 
Amplicon 5' Amplicon 3'
TCCTCTGCACATGAACTGCTCATATCAGCTCAGTTCATCCACTCACGAATTGTGAATTTATAACACACAAGATAGTCTCTAATAACCATCATACAACTTCAAGGAAACACACCTTTACATCTTTAAAATACGCAATGTATTATGAAAATATATCCTATGTATGATAGGTAATAGTAGAAGATAGATTGGGATATATCCTATATCCTATTGCAGTTATGTAGTATTAAATTCGTATATCAGAAAATATACTACAGGCTATGAAGATAAAAATGGTTTTCTTTAAATTCTATACCAATTTATACTCAATTTTATTAAAATAATAAAATTATCTTAAATAGTAAAAAGAATTTCCTATGCATTCATGGATTTTTAAATGTTAACAATAATAGTATTTTCTGATTCTTGGCTGACTAGTGTAGTAATATCACATCCATAAACTTGGAACAACGGTTCGTAAGCAACTCTATCGCGCTCTCCTAAATGTCCAAAGTTCTATTGCATCAATGAAAGCTTCGGCAGAAAGCTTTCCTCTGGCGTCGCCAACATTCCACAGCTGACTCGCGTCTATATAGTTCTGCTGCTCAGTCGGAAACCAACAGAAATAGCAATGAGTTCCGCGGTCAGCGAGTCCAAGCTCAAGGAGTACTACGATGTGGCCCTGAAACTGGTCCTCAAGTGCGGGCCTCTGATGCAGGAGGGCTATCAGAAGGCAAAGACGGAATACAAGGTCAAGGCGGACTTCTACGACCTGGTGACCGTGTACGATAAGCAAATCGAGGATATTTTGACCGAGGGATTGGTGGCCGCCTTTCCCGAATCCCTGATCATTGGCGAGGAGGAGTCGGCAGTTTCACAGCGCCAGGCGGAGCTGACTGATGCACCCACGTGGATCATAGATCCCATCGACGGCACCACAAACTTCATTCACCGCATCCCGCACTGCTGCATCTCCGTGGGCCTGGCCATCAACAAGGAGCTGGTCGTGGGCATTATCTACAATCCACCGGCGAATGAACTATTCTCCGCCTACAAGGGACACGGAGCCTATCTGAATGGCGAACCCATCCACACATCCAAAGTGACGACGGTAAGAGTGTGCTTATATAACCCGGGAGCAACAATACTATGTATAGCGACAAACCAGATAAACGAAATTTGACAAATAACCGTGCATGACATTATTCCTG CAGGTTTATCGGTGCACAGCAAAAAAAAAAAAAAAAGGTTGGCTAAACTTAAATTTGCATTTCGATAGGAATGTTTGAGAAATTTGATAAATGTGATAAAATAATTTATTATAACCGCTTTATAACAAAATTCCCATACAATTGTTATAACAACTTTTTGGAATGGAAAAATACAATCTGCTAACATTTTTTTCGGTGCACCGATATCATTCATTCGTATACCACCAAACAAAAATAGCTCTCCAAAATTAAGCGCATATACAATTACTCTCACAACTAATGTACCCCTTTCCAGATCAAGCAAGCAGTGATTGCCTACGAGATCTCCCTGATCCACGCCGCCGGAGTGAGGGACAAGAATGTGAAGCGACTGTACAAGATGGCCTCGAATGCCACGGGAACCAGGTGTTTCGGAAGTGCCGCCCTGACCCTGTGCTACGTGGCCACGGGCCAGTGCGATGCCTATCATGTGGAGGATCTGAAACCGTGGGACATTGCTGCCGGAGCCATCATTCTGACCGAAGCTGGCGGCACTGTGTGCCATACCAGCGGATCCAAATTCGATGTGATGAAACCGGATTGCGTGTGCGCCGCCACGCCGGAGTTGGCCAAGAATGTGATTAGTCTTATCGAAGAGGCCGGTCAGATTACAGAGTACACATTTAAGTAAAGACAGTTAAAGGCTCAGTATGCAAAGACAGTATCCACATTTATCACTTGTAAACTATTCTAATCGCGCAGATATTCCAGTACAATAAAACGACCTAAGTACATATAAGTATCGCAGAACTTTCAGGAGCTTGCGAAGAAAGCTTTACCATAATGTCAGCTGTGTTTCTAAATTAAGTATTAGTTTCTACTCTGATCAGTCGATAGACGAGCCATGAGTTACCGAATCGGCGAGGAAAAACTTGAAGTATATTACCAGGTATCCCTGGAACTGGTCAGGAAGTGCGGTCCGCTCTTTTTGGAGGGTTTCCAGAAACCGAAAACCGATTACGAAGTCAAGTCAGCGTTCTATGACTTGGTCACCGTGTACG
 

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