CR01853

CRIMIC CR01853

Gene Lst

CG8317

Information

Donor Location Cytology FBti Stock number
pM37-p02R:..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence ATGCGTTCGGCCTTGTCCGGCGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
AAAGTACGGCTTGGATCGCA AGGCCGAACGCATTATCGAA AAATCAGAGTACGCTGTCCG AGCTTTACGACTCCACGGTG
 
Amplicon 5' Amplicon 3'
AAAGTACGGCTTGGATCGCAGACTATCCGGCTGTGCCAACGGCGAGGGACTGTAGTTGAACTGATTGGGCGTCTCGATGTCCAGCAGGGCGTGGGGCACCTCCTGCTGCTGCGGTGCAGCCATTGTCGTCGTCATCGTTGTCGCCAGAATGACCAGGATCAGTGGAGCGAGCTGCAAGGAAGGAACAAGGAAGGAATGAAGGGAAGGGAACTTTGGTACACAAGTCAGTCAGAGGTGTGAATAGCTGATGGAGGCCAGCGCCAGAACCTGTCTGCTCTTAGCGCTCTTATGCTGCCAATTAGCATACAATAATTAATTTAAATTTAACCAACTGGCGGCGACGGGAAGATGTGATCCATGATATAATCGCAATCAAATCTAATTTATAGTCGCACGTAAAATATTCATGAGCACGTCGTCGCAATTTTGGTGGGCGTCATCGTCATCATCCAGCTGATCTCTAGGCGCCACCCAACCGGATGCATCACTGGCCATAGTCTCCTGGATCTCAAGCATTTGAATCAATCGCCAAGCCAAGTGTAATTGTTACTATGTTTTCGATGGTTTTTTTTTTCTTTTTGAATTATGTAAAACCTTAACCGCAGTCAAAAACAAATTAATTACAATCGGCGGCGGGGCAAAAGGAGAAGATCGAGATCGAGGTGGAGACTGTAACCGGTTGCGTTTCATGTGGTTCAGGGTTAAGAGAGCTCGACTTTGTAAACGGTATCAAGGCTCCACCAGCAGGATGGATGAGCTGCGGATGAGCTGCGGATGAGCGGATGAGCGGATGAGCCCAAGATCAAGTGCAAAGTGCCATGGGCACGAAATAAAGACATTTTACGACACTTCCTCATCGAGATGATCACAAGATCGAATCGAATCGAAATCGAAATCGAAATGGAAATGGAAACGGCTATAAGATATAGATGTAGAAATAGAATTGGAATAACTTGATTCGATAATGCGTTCGGCCT AAATCAGAGTACGCTGTCCGTTTTGCAGCCTTAATATGTGAATTTTTTTTGGTTTTTGGTCGAAAATGTTAATCGGCGAAACACGTTTCTTTGTTGGCCTGTGTTGTGTTTTGGCTCATAAATTTCGAACTTTGAGTAACCATAATACTCACTTGCCAAGTGTACGCAAACATCTTTGCTGGGTATGCGTTAGTCGAGTTGCGTTGAGTGGAGTATCCTGTTTCGAGTCTGCACTTAGTCACTTTTTCCAATTCGGATGAAAGATTGTAGATTGCAGATTGCAGATTGTAGATTGAAGATTTCACTCGCGAATAGTCGAAACCGTTCTGATCGATCGATTAGTTAGTCAGTTTGTTTGTTATCAGTGATCGCTTAACACCTTGTTGGCCCCCGGGGCTATCGATTCGCTTTGGTTGGCTTCGATTCTCGTTGCTGTCGCCGCTGAGCGTGCGCTTCGACTGTTCAGAATTTTTGTGACAATTCACTTTTATACGGCCCGATTCGATGCCCTCGCCCCGCGGAACTGGTTTCCCGACCCGGGTTTCGCTAAATATTGCCGATATCGCGGCGTGCGTAGCAGCCCCCTTTGCCACCAAAAAAAAAAAAAATAAATAAATAAATAAAAAAAAAACACAACCTAGGGTTCCAGTTCCAGTGTTTTGTGGCCAAATTGCCAAATTGGTGGCACTGGCTGCATTTTGGCCGCTCCAGCTGGTCACGTAATTAACGCTTCTGCGGCTTCCCAGGGATATCAATTCAATTCAAATCAAATCAAATCGAATCGAATTGAATCACATATGCGTCACATAAGCCGCGTTTGTTTGCGCTTGGCATTAACCGATCTTCGATCTTCGGCGTTAACTAATTAAACTTTTTCCATGACGCACTTACCAATCAAGAAGACACTAATCTCCCCAGTAGTTCGGATAAGCAGATAGCAGGATGAGAATGGCGATGGGCAACTGGCGCCACACACACACACACGGAAGCCACCGTGGAGTCGTAAAGCT
 

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