CR01847

CRIMIC CR01847

Gene TrpRS-m

CG7441

Information

Donor Location Cytology FBti Stock number
pM37-p23L:..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
long band OK

Images

Construct Design Sequences

sgRNA sequence TTTAAGTCTGATAAGCAACTAGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GCGAACGCTCAGTGTCAAAA TCTTGCATTCCCGGCTTACA AGACTTAAATGCTTTGGTTCAGC TGCTTGTAAGAGCCACCCTG
 
Amplicon 5' Amplicon 3'
GCGAACGCTCAGTGTCAAAATTTCGGCCGTTTCGAACAAATAATTTATTTTTGGGCTTTGTTCACACATTTAGCACCGCATTATTCCCAATATTTTTTACTCAAAAGTTTTGGGGTACAAAATTCATAGCTCATTGCAATGTTCCGATTTGGCAAGCTCCATCGCAGTCTGTCTAAGGTGGCCAAAAAGGGACAGACAGCTGTGTATTCCCAGCGCCGTTGCATTCAGGCGAGGAGTTTCTTGCCGGGATTGGCTAGTAGGACGGCTGGAACGCCCTCTGCGACGGCCCAGGTAACCGGTGGAAACAAGCAGCACGTACACCCCCATGGTAATTCCGTTAACAGTGGCCACGAAGAAGTAAGTGCATTCGTAGAAATAGTTTACATAACACAAGACTCTAATATATTACCCCAACCCAGCATAACACCCGCTGGCCGAGGAAAGTCTTTAGTGGCATCCAGCCGACGGGTTCCCTGCATCTGGGTAATTATCTCGGCGCCGTGCGCAAATGGGTGCAGCTGCAGAATGCTCGTGACGATGTCACCGTGTGTATTGTGGATTTGCACTCTATTACCATGCCCCACAATCCGCCGCTTCTGCGAGAGAACATCTTCACCATGGCCGCCACGTTGTTGGCATGTGGCATCGATCCCACCAAGAGCACCCTCTTTGTCCAGTCCGCCGTTGCCGAGCACGCCGAGTTCAATTGGATCCTTAGCTCCTTGACCACCATGCCGCGATTGGCTCAACTGCCGCAGTTCCGAGAGAAGTCCCGTCTTCTAAAGGATGTCCCTCTGGGATTGTACGTTTACCCGGTTCTCCAAGCAGCCGACATTATGCTTTACAAGTAAGTAAATATATTCCGTTTTTTGTTCAGAATGTAAGCCGGGAATGCAAGA AGACTTAAATGCTTTGGTTCAGCTTTACATATTTTTAAACCACCCTTTAATATCTGAAATGGTTTGCCATTTGCTTACTTATTATAGTACTTATAATATTCATATTCGTTTTTCGATCAGGTCCACTCATGTTCCTGTGGGCGCTGACCAGATTCAGCACATTCAGCTGGCCCAGCACTTGGCCAGAATTTATAACGGTCGCTATGGCGAGACTTTCCCTGTTTGCACCGCCATCATTGAGGATGGTGATGCCTCCCGTGTGCTGTCCCTGCGAGATCCCTCCAAAAAGATGTCTAAATCGGAAGCGAATCCCAAGGCCACCATCAACTTGTGTGACTCGCCCGACTTGATTACGCAGAAGATAAAGAAAGCCGTCACAGACTTTACATCGGACATTACTTATAATCCTGGCAAGCGTGCTGGCGTCAGCAATCTGGTCAACATCCATGCCCAGGTCACGGGACAGAGCATTAAGACAGTGGTGAACGAGGCCGCCACTTTGGACACGGCAAAGTATAAGGACCGTGTGGCGGAGGCTGTGGTCGAGCACTTGCGTCCGATCCGAGAACAGATACACCATCACATGACTAAGCGCAACGAGATGATCTACTTGCTGGAAGTGGGTGCAGAAAAGGCACGTCAGCAGGCACGCCAAACGTTGAACGATGTTAAACAGCGCCTAGGACTAGGAACCAGTGCCAACATTCCGGCTGCGGTGCATGTGGCTCCACTTCTGCCCGCACCCGATATTTCCAAAACCTCAGCAAGCAGGCGAATGTCCAAGGACTTCGACGGGAATGTCCACGAGCGTCACGAACGGATGTACGGATTCGGAGATCAGCCAAGCGGAGGAGCTGGTCAGTCAGCCACCGGCGAGGTGGCGGATATGCCTCAGGCCGTTGTACCGCGGGTGATCCGTCGGGCGCCCATCGTTCCCACTCAGTCTATGCGCGTAGAGAAACAACTAGGTAGCACTCGAACTCGGCACGTATTCCAGATGGACACCTGCAATGCTCCGCATATAGGGTTCAAACCGCCGCCAGGATTTTCGGCCAGCATGGTCACCGGTGCTCTAGCCAAGAACAAGCGCGCTACAGTTAAACCCGTTACTGGCAACCTGGGCTTCGGCCAGCAATCGCAGGGTGGCTCTTACAAGCA
 

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