CR01846

CRIMIC CR01846

Gene Ir64a

CG10633

Information

Donor Location Cytology FBti Stock number
pM37-p23L:..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence TGACTGTGGCGATATTATTCAGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
ACAAGGACGACTCGACCAAC TCGCCACAGTCAAAGGACAA GGAAGCTTTGGAGGTGCTTG TTGTTCCAAGTGCTTGCGTC
 
Amplicon 5' Amplicon 3'
ACAAGGACGACTCGACCAACTGCGACCGGCCACATCGATCCATTTGTGCCGCTCCAGCCAGTAGAATATCGGTGCTCCGCCAATGTCCGTCTCGTTGCGTATGAGTGCCCCAATCATGCCATCAAACCGGCCGTTCTTCACATATCCCCACGAAGTCGTCCTGGACAGGACGAACGTCCAGTTGAACATGTCGCGAACCGCCATCAGCAGATTGAAGTTGAAGCGGTTCATCGTGTCGATGTGACCGGACATAGTGTGCGTGAGGTAGTACATTAGGGTGCCTGTCATGTTCTTGTTGGTCACCTGGCAAATGGACAGATATCGAAAATATTGCTGCACTGTAATAAACTAGTACTTTATACGCACTAAAACCTGTAAAGGTGTCTATGCTACCTAATATTTTCCATCCAATTCTTTGCGAAGTTCAAACACTTTTGTAGTTGATTCAAAAACCAATGTGATTTCTGTGGTGCCCCTTCATTTTTCTCGAATGCTCCACTGACCACCCCCCGGCATGATGATGGATCTACGCATCCCACCCAATTTCCCAATCCTCACCACAACCATGCATCGCACCCTGGCGTGCTGCATGTCCATCCTGCGACGAACTGTGGACGTGGTCCGAAACCATGTATGCAACTGGATTCCCTCCGTCGGCGTGAAGCTGCCAATTTCGGTCAGATTGACGTGGCCGCCGTAGTTGAGTCCGGGGTTCCTAAGCGATGGTCCAAAATGGGAGCAAGTCGTTGTGGGAATTAGCAGCCGGGAAGTCGACAGTTGGGAAATTATGTCTGATGTGTTGATGGCAACCAGGCAGAAGTGCCACGCTTCTCTCTGGTATTTCAGTGGAATGGAATGAATGGTTTTGCGGTCAAGTGTTGGTGAAATGATAGTTTGTCCTTTGACTGTGGCGA GGAAGCTTTGGAGGTGCTTGAGGTCAATATGGTTGAACAGGGATGTGAGATACATTGATCTGGTTGGTAGCCATTGCATAATTAAATAGGCAAATATTTCTTTTTATTACAAAATGTCCTAATAATCAAGTATTCGAAATGAACAGAATACAGAATACATTTAATGACTATAATTAAATGCCTCCGAAACCGCAATTGCCAGGTTAATTTGAACTAACCACACATTGCTAAATGAAACACCAAATTAAATACATTGAATGTGTAAATAAATAAAATTTAAGAAACAATATAAGGTACAATGAAATAAGCAAGCAAATATATCAATATCAATCAATATATACAACAAGTCCTTTCAAGTGCTCAGTCATCAATGAACAATAAAGAGACAAATATGGCATAAAGTATTTTCGGTAGAGGACAACTTGATTCAAGTTAAGATTGAAGCTTGAGGTGATTCTGAAGTGGATGACCAGGCCAGTTAATCACTCACCAAACATCGAACAGAGTGTAGTGGGCTGCCTCGGACATTCGCTTGGCCAGAGTGAGTTCGGTGTTCATATACAGGTTCATGCCCCCCAAGAAGCTCTCGATGGAGGGCAGGTCCTCATCGTCGGTGGCCTGAATTTGCTGCCCGGGCGGCTCTTCTTCCTCCATCTCGCCATCATCACGTTTCCCCGATCCTTCGGCTGTCTGCGTCCTTCCATGCCAGGCGAAATCCTCTATCAATAGCCAGTGAAACTTCTCGCTGTAGAGCATCTTGGCGGAAGCCTTTGAAATGATTGTACTTGAGTACTTAATAAGGAGTCTACAACTAGACGCAAGCACTTGGAACAA
 

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