CR01844

CRIMIC CR01844

Gene eRF1

CG5605

Information

Donor Location Cytology FBti Stock number
pM37-p23L:..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence GCTTCACACCGCTTTAGAGTGGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GAATTACGGTGTTCGCGCTC TAAGGACCGGGTCAAGGGAG TCAGCAAATCGAGTGACCCC TGTGACTATTGTGCCGCAGT
 
Amplicon 5' Amplicon 3'
GAATTACGGTGTTCGCGCTCTGCTTGTGCTTTCCACTCCACTCCCCTTCTTAACTTCCCCCCTCCCAGCCTATTTACTCTGTGTGCATGTGTAAATGAATACTTTAAAACGTTTTTAATCGTTGCAGTGTATTCATCGCCAGCCACGTTAAAAGGAAGAACGTGTATGTGAATACGACCATCAGAGGATCTTAGCGAAGGATTCAGGAGCCCAAATTCTTAATCCCCATCCCACACACACACATATACTCGCACGTAGGCACGCTCTCTGTGAGAGGAAGGGTAAATAAAAGAAGCAACGAGCAAAATGTCTGGCGAGGAAACGTCTGCCGATCGCAATGTCGAGATCTGGAAAATCAAGAAGCTCATCAAGAGCCTGGAAATGGCCCGCGGGTGAGTCTTATCAATGATATACATGTCCTTTAACGTCCCCCCCAACGACTGTTTTGCGTACGTGTGTGGGGTATACACAATGCATGGCACGAAAACAACAACACTGGGGTGGAAGTGGGCGTACTGGACGTGGGTGTGGGTGGGGACTTGAGGGAGCGGCGAGTACAACCACCTTTCCTTGTTGTCGCCGTGTGAATCTCCTTTGCATTGTTTCCAATCTTGGCAAGATCCCCCGCCTCACTCTATCTCTCTTTTTTGCATACGAGCAACACGTTTTTGTCGAGTTTTTATCAGTTCAAACTAGACTGATTGAGAGACGGAGAGAGAGAGAGAGAGAGAGGAGTGAGTGAGTGAGTGAGTAATCGCCTCGTGGCTGTTGTGCGTATGCGTGCATGTGTGTGTGTGTTTTTCCCCCAAAATGGGCCAAGCTTTGTGGACCCCTCGCTCTCACTCATCCTGCCTCGCTCACTCCCTTGACCCGGTCCTTA TCAGCAAATCGAGTGACCCCCACAGGAGCATATCTGCTATGTATATACATATATATGTATATTGTTGTCAATATGCTCCACAATTGGAGCTAACATTACACCTCTTCCAATTGGAGTTGGCCACTGTTGCCAATTGGGATTCGCTTTGACAGATATCTTCCTATTGGTTTTCAGCCATATGCGAAATATATACACACATATGTATTTGTTTTTCTTTTTTTAAGTTCTGTGCTATTTTTTGTTGTATTTTGCATTCCAACATTTTGTACAAATTACGATTGGCACTCCTCTGTATTATAACGAACGAGAAAATGATTTGCATCAGAGAAACAAACGTTTGGAAGTTACAGAAACGTATTTGTTTCAATAAATTAATCAAAGTTTAAATTTAGTCCAATAACCGAATGTCAAGTTTAAAAGACATATGCCACGAAATTTCACGGCTAATTAAATAACTTTAAAGTAGGTTGCTATTATGTGCATCTGACGCGCAAATATACGATTGAATAGCACTGTTGCTATGGGAGAAAGAGCCATTTCCGCGACTTATGTTCTCCCTTGTTTGGGTTTCTTGCAGCAATGGAACCAGCATGATTTCTTTGATTATTCCGCCAAAGGATCAAATCTCGCGCGTCAGCAAGATGTTGGCCGATGAGTTTGGAACGGCGTCGAACATCAAGTCGCGTGTAAATCGGTTGTCCGTCCTCGGTGCCATTACGTCGGTACAGCACAGACTCAAATTATACACCAAAGGTAAGCTGAGCTAAGAAATTGTTCATAAACAATCGAACAAACGAACTCTAAACCGTATCACCAACCGCTTCAATTCAGTGCCTCCCAACGGTTTGGTCATCTACTGCGGCACAATAGTCACA
 

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