CR01835

CRIMIC CR01835

Gene BI-1

CG7188

Information

Donor Location Cytology FBti Stock number
pM37-p23L:..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence CCGATGGACAACTGTTGATGTGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
TGGCTGCAGGAAATACCGTT ACAGTTGTCCATCGGATCGA GGTTGGATACCAAAACAACACGT CAGCACCTAGTCGCCCAATT
 
Amplicon 5' Amplicon 3'
TGGCTGCAGGAAATACCGTTTAAAATGGTTTTTCACATCGAATTTTATACTGGAAGCTTTTGCAAAGTGATCAGCATGTTTTCGAGGATAATCTGATTGAGCTATTTTGGGCAACTGGCTCCGGCTTGAGCTATCTTAAACCATGAAGGTCTTGAGCTGGGTAAGGGGGCTATATAACAACTGATCGTTGGGATAAACCCTTGTCGCGGAAACATACCTTTTGCGTCAGTATAATCAGCAAACGGCGGAACATGCTGAGTACATCGAAGAACAAATCTAAAGCGTGCTGCACCACATCTCGATTTCCGTTTCGGCACTTCTCCACAATGTTCTGTGTATCGTAGACGATGAAGGCAGCCATAACGAAAACGCCGACGTAAAGTTGAGTCTATAAGGAAAATAAGAATTTGTTAAGTATTACAATTATGAAATATTCAGTTTTCAATGCCTTGGAATGGAAATTAGAAATTAGATTTAGAGGATACAAATTAATACGCAAATTAATGGGCACTCACCACTTGCACGAAGTAGGACTTGAAGACCATGTTAAAGAGACTCAGCAGCGCCATGGTGTTGATGACGCTAACCAGCATCCCACCCAGATAGAGGTACTTGCCCTGCTCGGCCAGAAGGGCGGACAAGGAGAGCGAGATGAAGGTGACGAAGGTGCCCGTAAGGGCAGACAGGATTATTGCCGGATTTATGCTGCATATATAGCCGAGGAGCGGTCCGAGCGTCTGGCCGGAGCAGAATCCGAAGGCGTAGAGCATGCCCAAACGTGTATAATAGTTCTTGCCGTCATCCTTGTAGAAGTGCAGACCCAAGACCAGGACTAGAGTGGCCACCGCCGCCAGGACTCCAAGATCGAGAAAGTCACGCATCTGAAGCATGGCTCCCATGGCCGTGGCAGCGGCAGTGCTGCCCAGGACCATGTAAACCTTAGACAGGTGCTCGCGCACATAGGGCTCACTGAAGGAAATAAAAGGAGGACTATTAGTTTCAAGGTCCATCGATCCGATGGACAACTGT GGTTGGATACCAAAACAACACGTCAGTGCAACGCACTTGGACCCCAACCTCTAAGGGGGTGACCCATCGGCATGTAATACTTACTAACGGTCACCGAGCCCGTTCATGAAGGTCTGGAAACGGTCGTTGATGTAATTCGCAGTATCTGCCATTGTAGTCTTTTTCGCACTTGCTCTTGCTAGTTTCCAGTAATATTACACATAAGTTTGTCAAACGGAGGAGTGAATTAGAACTGTTTGCACTGATGTTTTAAAACTGACTGTTTACTTTGTAAGGCCTACTCGGCTCTATTTTCTTGCCTTTTCTTTCTGTATTTCACGAAATCTGTTTTATCAGCTTTTAGTGTTGGATAAGTTGCTATAGGTTAGTGCTGACGACGTGGTCTTTTTACAGCTACATTTAGATTTGAACTTTATGCTCGTATTCATTATTAAACTCACCGTATACACATAAATACTTGTACAACATACTATAAATTAAGTTTAACATATAATCCTGTTAATTGAATAAATAAATAATACTCCAGTTCAATTTTGTATAAATCTTTTGGATTTTATACCAGCCTTTTTTTTTTCAAAACTAGAACACCGTCAGCACTGTTGATAAGGTGGTGTTTTCGACAATTGCCCACCTGAGTCAGTGTTGGGTAAACGGACATTTTTCGAACTTCCAGATGATTCTATGACGTGTTTTTCGGTTGCGCGCGCTGCCTACTTTCCATTTGAAAAGCAACGAAGTTCGAAACGACTAGTAAATGTTTTTTTTATTTCGCGCTAGATCCAAAAACAAGGTAATTGGCGTATTTTCTAAAAACATTGTACATGGTAAATAATAAACTATGGCAAATTGGGCGACTAGGTGCTG
 

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