CR01833

CRIMIC CR01833

Gene CG12004

CG12004

Information

Donor Location Cytology FBti Stock number
pM37-p23L:..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence CAGATTTACTGGTTTACAGTTGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
TCGGAGCAATGTTCGTGTGA AATTTATTGCGCACGCTCGT TCTGCCATTAAGTAACACTTTGC CTTTTCGTAACGCTGAGCCG
 
Amplicon 5' Amplicon 3'
TCGGAGCAATGTTCGTGTGAAAGTCGAGACGAGAACGTAAACATTTTGCCTGCCTAGCTGTCTGTGCTGAGCACTTCAACTGGCCGCACAATAGCTCCTGAACCTAGCCCAGTTACACCCACGCTAATCGGTCTTCCTCGTTCTGGATTCCATTGCAGCATGAGCACAACCGCCGCCAGTCTCATCGAGGCGGCTCTAAATGCCAGTAGTGGAAACGCCACGGCTACAACTGCGGGATCGCTTGAGGCTACAGGGAAGCCCAACTACGTGGTGCAGGTAAAGAACACTCCGATGAGCACGCCGCTGGACCCGCTGCTCCACGTGGGCGATGGCATCTTCCTGCAGACAAAGACCGCCCAGGTTCTAGCTGGGGTGTGCGTCTGGGCCGCCCTCTTCATCACATGTCAACAGGTGAGTTAAGGCGTCATTGGTCTGAGTTGTGTACTAATGGATATCTTTGGCAGATCTACCAGCACCTGCGCTGGTACACGAATCCACAGGAGCAGCGCTGGATCGTGCGCATTTTGTTCATTGTGCCCATCTATGCCACATACTCCTGGATCAGTCTGCTTTTCTTCAACTCGGATAACGTTTACATCTACTTCTTTACGGTGCGCGACTGCTACGAAGGTTCGGCTGATTTGATTTTGAATGATTTTTGCCCATCGACTAATTGCTAACCCCATTTCCATCCAGCCTTTGTCATCTACAACTTCTTGTCGCTGTGCTATGAGTACCTGGGTGGCGAGGGCAATATTATGTCGGAGATTCGCGGCAAGCCCATCAAGACATCGTGCCTGTATGGCACCTGCTGTCTGAAGGGCAAGACCTACACGATTGGCTTCCTGCGCTTCTGCAAGCAGGCCACTCTGCAGTTCTGCCTGGTCAAGCCGCTGGTGGCCTTCATTATCATCTTTCTGCAGGCATTTGGCCACTACCACGATGGCGATTGGAGGTAATGTGGTTTATCTTTAATGACCCGCGTTATTGCATCTGAAATGGTGGCATAAAAACAACGAGCGTGCGCAATAAATT TCTGCCATTAAGTAACACTTTGCAAAAGAGAGAACTTATAGGTACATATCATTCCCACTAATAATATCTCCTTATCTAAATTGCAGTGCTGATGGCGGTTACATTTACATCACGATTATCTACAACATCTCCGTGTCGCTGGCGTTGTATGGCCTCTATCTGTTCTACTTCGCCACGCGTGATCTGCTGACTCCGTTCGAGCCCGTGCTAAAGTTCTGTACCATTAAGTCCGTCATCTTCTTGTCCTTCTGGCAGGGCGTGGGTCTGGCCATCCTGGAGAAGGCCAATGTTATCTCGCCCATTGTAGACAGTGCAGGCACTGTTACCGTGGAACCCGGTACCGTCTCCGCCGGATACCAGAACTTCTTCATCTGCATCGAAATGCTGTTCGCCGCCATCGCGCTGCGCTATGCATTCCCCTATCAGGTGAGTTTTGGAATGTCGGAGTGGGCTAGATTTTACGCAGCTATTTACGATTTTTGGTGATTAGATAGTGCTGACTTGAGTGCTTTTTTTCGGTTATTTCGGTGTTTATTAAGCGGCTTTCCTTACACTTGTTAGATATGTGAAAGGTATTCGTGTAATGCTGACTTGGCTAGAAGAAATCAGCTAAAACGAAGTGCTTGCAGTAAAAATAACTTAAATGTTGTATGCTCTTTGTAGGTCTACGCACGCAGCTGCATCAGCGATGGCCACGGACGGTCTGTCACCATGCAGTCCATTTCCAGCAGTCTTAAGGTAAGCTGGAATATTTCCCTGGGAATTCCCGTATCTAAAACTCCCGCAATCCTTCGCAGGAAACAATGAATCCCAAGGATATTATGACAGATGCTATACACAACTTCCATCCGCAGTACCAGCAGTACACGCAGTACAGCTCAGGTTGGTTTTTACTCGCTTTCTCGTCTTTTTAGCCCAACTTCTTCTCTTTGTGCTCTGTGTCTGTGGCATGTTCGTTCTTTTCATCGAGCCTGCTTAACTTATCTTATCTCTTGTGTTCGTCGCTCTCATTTAATGCATTTCGATGCGAATTTCCATTACCATTTCCATTCCATTATCGTTATGTTTTTCACACTTTTGTTTTTCTTTCTTGTTTTCTTATCTTTTGCAGAAGTAACCTCGGCTCAGCGTTACGAAAAG
 

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