CR01830

CRIMIC CR01830

Gene l(3)72Dr

CG32154

Information

Donor Location Cytology FBti Stock number
pM37-p23L:..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
short band OK

Images

Construct Design Sequences

sgRNA sequence AGTGCGATTAACGCATGAGCGGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GGACGGGCAAGGAAAGATCC GTTAATCGCACTGCATTCTGC AGCGGGGAGAGGTGTTCATA CCAGTTGGAGCCCTTGAACT
 
Amplicon 5' Amplicon 3'
GGACGGGCAAGGAAAGATCCATTGGTCAAGTGATTCCGATCAAAAATGTATATAGTTAATAGGGTCGGAAACGCTTTTTTTTATAGCTTTGAAGTGTATTAAGTATATCATCTATTTATTATACATATAATATATAATTCATTTTTTTTTTTGGTTTTTAAACGAAATTATTTAATTTACAATAATTGACTAGCGTACTATTACCCTTTTTAGGAAACGTAATCATAATATCATAATTCATATATTTATAATTATAGATTTATAATTCATATAAATCATTTACATTACTTAGCCACTATAGCTTGGCTCATGATAAATTTTATCTTACTTATAATTCCAACGACAATGGTTGCAAAAGTACAATATATTGGCTAGGTTATTGTTTTTCTCTCGCCGACTCGAATGCGAGACTCATTTCTATCTGCTCGTGCCCGAATAAATAGGAATGTATGGCATTTATATCTGGCCACTAGCTCGTAGTCTGAAATCCGAAGAAGACGATGGCGGAAAGCGCTTATACCGATGCGCCGTACCTCAACCGCGTACCGACAGTTGGTGTGATGTGCATCGATATCGCCACGCAACTGCAGCAGAACTTTAGTGGTGCATATCACAGCTATTTGGCTGCATCCTATGTCAAATTTTTGGAGGCATCCGGTGCGCATGTGGTTCCCATTTGGTAAGTTATAAATTTTCAATCATTTTGCAACACTTTGACTTTTATTTTACCAATTTTTACATTGCTAGGAAATAATATGACAAATATAAAAGGGGACAGAATCAATTCGTAATCTTTTTGTATGGTTTAATGTGTATAACCATATTTTCATTAGCCGCAAGCATTCCAAGCATTTAGATCGAAAGTCACGTACCAACAAATAGGGCAAAAGCGATTCGTATGATTGGGGTGTCTCAAATCTGCAAAAGCATCATGTGCATCGATTTCGCCAAGTCACTGGGCCACACTTTATCTGCCCGCTGCCAATTTGAAGCACTTGGATGCCATTGGGGCATTGGGTGTGCCCATCTGGTTACCAATAAACAAATTGTATAAACAAACGATGCACGAGAATTAATTACCTCATCTGGCAGTTTGCCAAAATAAGTTTTGCAGAATGCAGTGCGATTAAC AGCGGGGAGAGGTGTTCATATTTAAGGCAAATTTATTGTAGTCGGCAAACGAAAGTGCTTTCGATTTCATTTGAAAACGCGGGGGCATTTAAATATCATAAACACATACGTTGAATAGTGAAATTTTTTAATTATTTATTTTTAGGATCGGCCGAGAACGAGCTTACTATGCGTTGATGATGAGCCAGTTAAATGGCATTCTTCTGCCAGGTGGAGCCGTGTTCATCGACGAGGCTGATAGGCAAGCCAATCCGGATGTGACCAGCGATTGCGTCCGGAGTGCCGAGCTCATCTACCAATTGGCTATGGAGCGTAATATGCGGGCGAAGAAGTTGGATGATCGTGGCGCATACTTTCCCGTTTGGGGAACTTGTCTTGGCTTCCAGTTGATCCTTATTCACGCTGCCGAAGCACCGAATGTTCGAATCGCCTGTCAGCCCATGAGGGAGGCGATGCCAGTTACTCTGACCGATGACTATCAACAATCACAACTGCTTGGAAGTCTGCCGAAATCGGTTGCCGATGAGATGGAGAAGCACCCATTTGCCTGCCATCAGCATCGGTATTGCATCACTAAAGAGAGTTTGGAATCCTATGGTCTAGCTAAGGATTGGCATCCCCTGGCCACTCAGAAGGACACTTCAGGACTGGAATTCATCACGATTGTCGAGCACAGGCGTTTTCCCATCTTTGGATGTCAGTTTCATCCTGAGCGCGCTGCATTTGAGCAGCTTTTCAACTCACCGGACAAATGTTACATGGCGCATTCTCGCATGGGTATCGATTTGTCCCAAATCTTTGGCAGTCGGTTTGTTGACTTCTGCCGCCGCAACAACAATCAGTTCGAAAGTGACAAATTAAAAACCAGACATCTCATTTGGAACTGGCAGCCCGTTTTCTCTGGCAAGTTCAAGGGCTCCAACTGG
 

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