CR01804

CRIMIC CR01804

Gene ssp2

CG9028

Information

Donor Location Cytology FBti Stock number
pM37-p13L:..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
long band OK

Images

Construct Design Sequences

sgRNA sequence ATCGAGGTGCGTGTCACACCCGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
TTTACTTCCTGCCCAGCCAG CACGCACCTCGATTTTCTGG CGGTCTGCCCAAATCGATGA TGCAATACGAATGTGCGCTG
 
Amplicon 5' Amplicon 3'
TTTACTTCCTGCCCAGCCAGCGAAAGTCAATCAAGCAATACGCAAATAAACTATTTGCGTACAATTAAATAAACAATTACCACCGAACTAAAGACGAGGATCAAAAATCGATTAGGCGATTTAATTTGAGTGAAATATTGTCATGCTGAGCTATTTTTAATGATTTTCCAAAGTGGAATGCCAGAATTCCAAATATTGCTAAAAATATAAGTTAAATAAAACTATTTACAACAAAGTTCATACTTAATAGTCATTCCTGTAAATGTATGCAATGGTTAAACTCAGCATAGATAAATATTTTAAATAGCTTAAATAATATATACAAAATATTCTTTTAAGCGATAACAATAGTAGAAGTGGAAGTAAGAAAATTACGTTAACAAACAACAATTACTTATAATTATATATGAAAGCTTCAGGGCGTTTATCCAGTAAGTTAAAAAATAGCTAAATGCTAACTTAACGGTAAAATATCAAATAGTGTGCTGAACACATCGCTTTTGCGAAGCGTCCTTTGGATCTTTACTAACACTAAAAAAATTGTAAACTCCCGGAGATTGCTAGTAGTGTGCACGGAATTTAATTACTGGGTTTATTTCCTCAAAAGATTTTGTAATTTTACTAGTTTCCTGAAAACTTTGCGATGCAAATATTTGCCACTTTCAAATCCCACGAGCCGGACAGCCGCTTGATCCAAAACTTCAAAAAGCACGTGAATTCGCAGTCGCCACTTGACGATTTGCGAAAAAATCTAAATGAAAAAAGTAAAATTCGAGAATATGTACAAGAGCTGATTGGAAATGAGCAACAAATGGGATCCGTGCTGGACACGATCTCTTCCACCGAAGAGGACCACCGCGTGGCCACTGTGACCGGATGGTCCACGGTGAACATCGCCAACTGCGCCGGCGTTTCCAATTCTTCAATTTTTGTGGCACTGAAGTGGGTGATGATGCCCATTAAGGTGGAGATATTCAACTTCAAGCGAAGTGGACCGGAAGATAATATGGCACGTGGACAATTTCTGTTGGACAACCTGTTAAGCGCAAATCGCCTAAAACGCTTGCAAGAGGAAGTAGCAGTTATTAACACGTGCCAGAAAATCGAGGTGCGTG CGGTCTGCCCAAATCGATGATGTGTGCCCAGATAACAGACGAATTTACAACTGCCATTCAGGAGGCCCTTAGATCACGCTATGAGGTGGATTCACGCACCTTAGATCTCTCCAGGTTCCACGCCAGTCCGGAGTTGTCTCTGCACTTTTGTCCGCTGCATATGGTCAAGCTGCTAGAAACAGTGCTAGTTCTTTCAAACCATTTATTTCCCCATGTGACCAGCCTAGTACTGAGCAACAATTATTTGTGTTCCCTGAAAGCGTTTGCGGGAAATTCCCAAAGTTTTGCCAGCCTAGAGCGGTTGGACATAAGCGCAAACAGGATTCAAGATTTGGGAGAACTCAATTACATCAACAAACTGAGTTGCAAGACGATCTTCTTGGCAGGAAACGGTTTGGCCAAGCTAAGCGTGGACGTCATTCGAAAAATGCTACCACAGTTGAAAAATGTTCATGGCTGTGTGCAGTTGGGGGAAAGTACAGAAGCTGTTGATAATATTCCGAAGTCTCAACAACTTCAAGGCGGTGGGACTAATGGTTTAAAGTTTTGCCAGGACTTCATAAGTTCATACTACACTTTCTTTGACGATACCGAAGAGCGTTTCAAACTTAAGAAGTATTACGACGACCAAGCCATGTTCTCGCTTAGCGTGCCGGTACAACTAAATTATGTCTACGGCTACAAGCTGTACAACAGAAACCAGAAGCGCCAGCATTCCTCGTTTGCACAAAACGCCAAGCTGCAAGTGGGTAGAGCTGCGCTACTTCTCGCTTTAAGCCGCTTGCCTTTGATGCATACAGACTTAGAGAATGTCGGGCTAGACATCCAGGTGTTCACTTCGAGTCTACGCATCTTTACGTTGACGGGATACTTTAAGGAGATATCATCGGATACTCTGGAACCACGACGCTTTCAGCGCACATTCGTATTGCA
 

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