CR01769

CRIMIC CR01769

Gene barc

CG6049

Information

Donor Location Cytology FBti Stock number
pM37-p03L:..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK seq verified

Images

Construct Design Sequences

sgRNA sequence ATTGGCCAGCCTTATGCGCTCGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
TTCAGCGCCAGATTGACACT GCTCGGCATACAGATCATGC ATACCGAGCGGGAGAAGCAA AAACATGCGTGACTGGTTGC
 
Amplicon 5' Amplicon 3'
TTCAGCGCCAGATTGACACTCTCCACCTGAAATAGAAACAAACTAACTAGTAAATGTTTCTTCTAGGTACCACAGGCGTGTCAAGGCCGAGGACAGTGACGAAGAGATGATTATTCCCGACCGTCGACGCAATAACAATCGTCGCAACAACCACAGAAACCGTCAGATCAAGAATCAGAATCAACATCCCAATGATGTGAACTACCAGCCAGCACAGCAACGCCAAAGGAATCGTCCTCGTACTTCTACTGTTGCGCCAACTACTACGCAATCGGTTAGCCAGTCTAATGATGTCCTGGCTCCCTCACCCACGCCTCAGCAGATGACTCAGTACGATCGTAAATTGCAACGTCAACGGGAAAGGGAACGTCGCCGACAGCTGCGTCGGCAGGAACGCCAGAAGTTGGCTAGGGAGCAAAAGCGCGAAAGGCAACGTCGCCTTAAGGAGGAACGCCAGCGATTGCAGCGGGAGGAACAGCAGCGCAGGCAGCGATTGCATCACGATGAACCCAAACCCCAAGTGAACCCCCAAGTGAAGCCCCAAGTGATAGATGAACTCCGTCAGAGATCTGGTGAGGATTTGGATAAGAATCAAACGGACGAGCACGAACAGAAACTAAGAAATGAACAGGAAAAAAAACTAAGGGAAGAACAGCAAAAGCAACGCGATGAACAGGAACAAAAGGACAGAGAGGAGCAGGATAGACTTAAACAAGAGGAAGAGCAGGCCAGAACGCATCAGAAAGAGCTCAAGGAAAACCAGGAACAGCAACTTCGCGAACTTAAAGCCAAGCAGGAGCGAGAAAAGCAGGAACGGGATTACCAGCAGCAAAAGCGTGAGCACGAGCTCGAGCTCCTGAAGCAACGGCAGGCTGAAGCAGACCGCCAGCACGCAGCGGACGAGGAGGCAGAGAAGCTACGACTGGAGCGCATCCAAAAACAACGCGAACTGGAGGCACAACAGCGACGAGAGCGCGAAGAGCAGCGCCGCAAACAGCGCGAAGAACAGGAGGAACAGGATCGCCAAAACCATGCCAAGCGTCTGGCTGAAGAGAAGCGACTGCATGATCTGTATGCCGAGC ATACCGAGCGGGAGAAGCAACTTGCCGAGGCCCATGAAGCCAAGCGACTTGAGGAGCTGAAGCTGCAAGAGCAGCTTAAGAAACAGGAGGACGAGCGGCAGGAGCAGATTCGACGCGAGCAGGAGGAGGAGGAAAAGCGCCTGGAACTGGAGAGGCTTGAGGAGGCTCGCCGCTTCGAGGAGAAGGAACTAAAGCGCCTGCACGAGGAGAACCAGCGGCGCGAGGAGCAGAAGCTCCAGCGCGAGCGGGAAATAGCACTCCGCGAGGCTGCGGAGAAAAAACTAGCTGAGGAGGAGGAAATGCTGCGCAAGGAAGTGGCTGAAGAGGAACGCAAAGTTAAGCAGCGACTAGAAGATGAAATGCGACAGGCAGAAGAGGCGCGTAAAGCCAAGGAGGCCGAGGAACGGGCTGCCGAAGAGGCCAAGGCCGCCGAGCAGAAGCGACGCGTGGAGGCGGCCAAAAAGAAGGCCGACGAGGAAGTCAAGGCTAAGTTGGAGGAGAAGAGGCGTGAGTATGTGACTCGAATCTCGGCGCTGAGTCCCGAGGACCAGAAGAAGTTTATCGAGATGCGCAAACGGCGCAAGCAGCTGAAGGAGAAAAAGGAACGTGACCAGCGGGCAAAGGAACTGAAGAGGATACAGCAAGCCATGCGATTGGATGACAACGAGTAGAAGTCCAGGCGGCAGTCAAATCGCCACGCACTAACCAATACTAACCGATACATATCGATCACAATTGGTTCCACATACGCACCGTTGCCCAGTCGATCCCTTTTCGATTGTTTTCTTTGTGCTTTAATTAAAACAAACCACAAGATGAACACGCAAAAGTATTATTTATCAGAAGAGAAGGAAGAATGGAGGAACATCGCTGCGAAGCAGATTTTGTCGGTTAATTAATTGCATTTCGATCGAGCAGGAAACCCAATCTCCATGCGCTTCGCTAGCTATTTTAGTACTGTACAGTAAAGATATACTATATATAGGTCTAGGATATGGACACGCCACATACTCACGCAGCACACAGGACACACCGTAAACACACGGCAACCAGTCACGCATGTTT
 

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