CR01760

CRIMIC CR01760

Gene GAPsec

CG5978

Information

Donor Location Cytology FBti Stock number
pM37-p03L:..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence ACTTGCTGCTAAAATCCACACGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
AATGAGACGATGCCACACGT AGTAAGCCCATCTGGAACTGC CACGGTATGCATGAATGAAACA TTACCGCCCAATGCACTCAA
 
Amplicon 5' Amplicon 3'
AATGAGACGATGCCACACGTTCATCGGTATTCTTTTAACGACTGACCAACCTATGACTTCTTTAACGTTCTGACGTTTATCTAATTGGGACCACGAAATGTTAAAATTAATTCGTTTATTAATGACATACAAATTATAAGTCATGTAAAAAAGTGAAAATGCGTGTACTTATTGGACTTCCTGTAAGGGTGCGTTTCCAAGATTGGGGCTAACTAAAGTATAGTTGATTGCTTGGTTCTTTCAGTTGTTAAACTAACTAGGGGGCTTTGCAAGCATTTGGGCACAAGGATATATTTCTGGAGGAATGCTACGCCAATGATCCGGCATGAGCAATCACAACATTAATGTCAATTGGCGGATAGTTTTGCAAGAGTTTCACATTCGAGGCAAAGTCGTTTTCCAAAATTGCTTCCCTTTGGATTCTGCAAAATTTCATATTAGTTCTTGCTTTATACTAAAGCTAAGTTTACTTACAATATCATTGAACAGCATATTTTTATCAGAAAATCAAATCGCTGTTCGTCCGCAAACACGGAATCCCAAATGCGCAGTACATCTGGCAGTGGAAACTCCTGCGACAGGAGCAGTGTGAGCCACCTGAAGCTGTAATACTGCGGATGCAGCTCTTGGCTCCTGAGTAGCTCGTAAATGCTTAGGTCCTTTGATTTCAGCATATTCGATAGCCTGGCCATCATGAACTTGATGCCGCCTTCTGCGTCGTCTAGTGTTTTAATAAAAAAGTCTCTTATCTCGCTCATCAGGGCGGTAAAACAAAAGAAACAATCAGCCTCAGCGTGGGCGCGATATGTGAGGTCAGGATCGGAGGCCATAACATAGTAAATGGGTCCCACGATTTCGTTCATGCCCTGGACATATCCCTGGCCGGGATTTAGTTTGGCATAGATGAACAAAATGCGTTGCACAACTTCCCAGTGAGCCTCTTGGCCCTCCTCCATGGCGGCATAGTTTTCAACGGAGCGTTTTGTAATCAAATTTATCTATATAGAGCACACATATTCATTAGGTTTGGGGCAGTTCCAGATGGGCTTACT CACGGTATGCATGAATGAAACAACACATTCTGAGCTTATTGTCTAGCACAATTCCGATCTTGAACTCACCTTTGTCATGCCGAGGCCCTTGCGTTCCACATTCGCCGAACTTAGAACGGCGGGAACCACCCGTTCGTGGAGCCTTCGTCCATGCTCTCCTTTGCTGTGGACCACAATCTCGCAGGGATAATCGGTGGGCTGCTGGAAAAAGGATATGTCCGGGCACAAGCGGCGGACATCCTTGTCGATTTGCAGTAGAAACTCGTTATCATTGAAGAACGTGTTCCACGCGCTTTCGGGTCCCTCGCTCAGCGGATGATCTTGCAGGCCAACGCCCCCCGAATCGACTTTGTCCCCATCTCCTCCGTCTACGCTAGCTCTATTGCTCGAGTGTCCGGGGGGCAGGACTAGCTCCTCGATGAACTGTTTATACAGGGCTCGCTTTTGGGCCAAGGTTGTGGTCCAACTACTGCGCCTAGGCCCCAAATAGCCAAGGAGTAGCTTCCAACTGAGTGCTCGAAAACTCTGCACATCGGGCACACCTGTGGAGTGCTCAGACTTCAAATTTTGAACATTTCAAATGCACTCTTAGCTTTCTACCATTAAATGCCAGTTTCCTGAGTTGTTTCAGGTCAATTAAATCCTGTGGCCCCGCCAAAACATCCTCAAATTCCTTGACTCTGCATTTTGGTTAGAGAAAGTCTTTGTTTACGGAGAAGTTTGGATTAGCTTAGAATAACAGCTTACCGCGCCTTGAAAATAGACATGCTGCGGCTTTATGTGGGCGGGGCTATGCTGAGATCTTGCAGTACCCCTATGTGTAATTAATTAAAGCTTTATCTCAGTTTTATGCTGGGTGAAATCTGCAGGTATAAAACACAACAAAACAATGGGCGTTGCCAGATGGTTCGAGCTCTAATCGATTATTCTCTGCGACTATCGATTTTTGAGTGCATTGGGCGGTAA
 

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