CR01530

CRIMIC CR01530

Gene wapl

CG3707

Information

Donor Location Cytology FBti Stock number
pM37-p0X:2153065..21530672D5Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence AAATACCAAGCGCCCAAAGGCGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
CCCCTTTGACCTAGCAGCTC TGGGCGCTTGGTATTTGCAT AGGCGGTTTGGAGCTCAATA CAGGTAACGCAGGTGCTACA
 
Amplicon 5' Amplicon 3'
CCCCTTTGACCTAGCAGCTCGGCTCCCAACGATGGCTGCGCCTCGTTGAACGTGTGCGTCAGGTTGATCAGTACCTTGAGCACCGGCACCAGCAGCTCGCGCATAGCCACACCCGGATGCTCCTTGTTGCTGCCACCACCATCCGAAGGATGCAGCATTATTTGTCGGCTGCAAAGACGGTACAGTTGGCAAAGCGTCTCCACTGCTTTTCCCTGGCCAGAGGTGAGCATGTAGCGCTGGTTCGTCTCATTGTGCTGCGTCACGTTTTCGAGGACTCGCAGACAACGCGCCACCGTTTGCATGTTATCCAGCAGCGTCGGCTGCCAGTCAATCTCCGTGTCGCAGGCAATCACCGGTCTGCAGAAGTCCGAGATGGTCTTGATAATGTGCTCCAGGCCACCCAGCTTGCGCAGATCCTCTTTAAACCACTCGCCCGCGCGCTTGGATGTTAGCGATAGCAGCGTTTCCATTGCCAGCGTGCCCACAGTCAGAGAATCAACGTTGAGATGCGTTCCCTTGCCCTGCGCCTTGATCTCCTCGCACAACTCGCGCACCTTCTGCTTGTTGCGGTCGTAGCCCGCTCTATCCGGGTGCCCAGTCTCCGTGCTGCCACCCACGCCGTCCGCCTCCAGAAGGTTGATCATCAGCTCCAAGGAGTCGCGATCCAGGTCCATGTTGAGGCCCTCCTGGGAAAGAATGTACATGATGGCCGAGGTGCACAGGCCTAGGCTGAGGTCCTTGTTGGCGTCGGATAATGCCTACAGTTAAATAGTCAGTTAATTAACAAAGAGGTTGATACTTTTCCTAAAACTTTTTTTTTTGTTATTCACTTTGCAAATAATCAATAAACTGTACTGATTTAAAGTGGAAAACATTACCAAGTTGATTAAAGTACAACTGCAATGGTTTAATAGTTTATACAGGCTTTTAATACAGAATAACAAAATAACAACACAGCTTTCAAAACAAACTGGCTATTCTGGGGACAGCTTTGCCGAAAGCTGATATTTTGAATTTGATAAGATTCAATTGCATAATCAAACTATGCAAATACCAAGCGCCCA AGGCGGTTTGGAGCTCAATATATGTAAGAATTGGGGATCTAACTTACCTTGAAAAATTTGGTGACCACCCCATGGGCACGCACGTGCATCCGGAAGGCGGGCATCATACACTTGGCGGCTAGCTGAAGGGCGGAGAGACACCGTGTTGCCGGGGGATTGTGCGGCTGAAGTGCGTCCAGGATGTACTCGACGTCGTCGTCCATTTCCTGGTACTCGCCGATCTCCTGAATTTGATGGGCCGTCTTAACGTTACGCACCACTGGATAGTAGTCCTTAGTTTTGCGGTCGACCCGAACGGGGCCGCCACCGCCCGTCGCTCCACCTCCGCCTGTCGTTGCCCCTGTTCCACCAGCGGAAGCACCAGCTGCACTACGCTCTAGGTCAAGCTCTCCGGCAATTGGTTCCAAATCCAGGTCAAAGGCATCGGAACTAGTGGAGGTGGCAGGTACTCCCTGTTTTCCCGCCAGTCGCGAGATCTTTCCGAGGGCCGGGCTATCGCCGCGCAACGTGGATGCGCTGCTGCACGCACTGGAGGAGCCATTGTTGTTCGACGACGGACTGTCACCGAAATCACCATCGGACTTGCCCGCCGCTAAATGATGCAGATGATCCTTCGCCCCAGCGACGCCCACGCCAGATGCCGAGGCATTGCTGGCATCGCTGTTGGTTCCACCGCCATTCGCTTCTAGCGCAGCATCCCAACTGTGCTTGTACAGATGTCGCTTGGCCACTGTAGCCTGTTCCGCTTGATCGGATGGCACCAGAGCGCGGCTTTTGAAGATGCTGCCTTTCTTCTTTGAGATGACCAGCTTGACAGCGCCCCGCTGTTCGCCAGAGGACTCAGGTGGCGCCGCCGCTGCTGCTCCTCCCGCTCCGGCGTTCGCCGCGCCCTGTGTGTCCAGTGTCAATGTTCGCTTAAACTTATCCTTAAACTTGAAATCGCGGGGTGGGGGCGAGGAGGAGGACCCATCGTGCTGCAACGAGGAGTTGTTTGAGTTGTTCGACAGTTCATCCGGATCAGGATCCGGATCATGGTCTGGCGAATGTTCCCTGTGATCTGTGTGCGGAATCTTGGCGGGTGTGGGCTGCTCCTCCTCAGCATCAGGAGTTTCCTGCTCTTCGGGCACTGGCTCCTGCTGTAGCACCTGCGTTACCTG
 

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