CR01522

CRIMIC CR01522

Gene awd

CG2210

Information

Donor Location Cytology FBti Stock number
pM37-p03R:..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence GCCCCACGCGGGGTTGCCATCGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GGCCATGTCAAGGAGTCCAA TCGGACCTAACCTCAAAGGC GGCAACCCGTTTCCACGTAT GCAAGAACCCTGCAGCATTC
 
Amplicon 5' Amplicon 3'
GGCCATGTCAAGGAGTCCAAGAAGGTGGCTTCACTTGAATCTCAATGGAAGGCTGTGTTCTTCGGCCGATCCTTGCCAACTCCCAAATTAAATAAGGGGAAATTTCTTGAAATTCGGTATGTCTAGGGAAATGCATTGTCCGGCGGGAGCTCCCTTTTAATTGCATCGCTTTTTCCAGCTCCACATGGGATTCTTACATAAGCTACTGTGGAAGAGGCTCCTCTATACCCAGAGGATGGGTGCTACCGCCAACAAAGCCTACAGCTCAGTGGATAGGCTATCGACTATCCTTAACTTAATATCATAGAAACCTTTCCAAAGCTCACACTCCTTATAAAGAAGCAAGATTATATAGTAATTATTGTGGAATTTTATTTCTTTAGAAGAATCAATCTGTATTCAGCTTAGACGAGGGCAGACAGTTAAAGTAGCCGTCTATTCGTAGATCCAGTCCTTGGCGGCCGGGGTCCAGGTGACCAGCTCCTTTTCGTTGAACCACAGGGCGATCTCCTTCTCGGCAGACTCGACGGCATCGGAGCCGTGGATGATGTTGCGTCCGACCTGAATGCAGAAGTCACCGCGGATGGTGCCGGGCAGCGAGTCGGCGGGGTTGGTGGCGCCGAGCATCTGGCGACCGGTCTTGACCACATTCAGACCCTCCCACACCATGGGCACCACGGGGCCGGAGTTCATGTAGTTCACGAGTCCGGGGAAGAAGGGGCGGGCGGACAGATCAGCGTAGTGCTTCTCCAGCAGCTCCTTGGAGGCCTGGTTACAAAACGGTTCGTTATCAGTTAATTGCAGTTCTGACCTCTAAACACATGTCATTCCGCGATGACGCCTGCACCCAGCCTTTGAGGTTAGGTCCGA GGCAACCCGTTTCCACGTATTTATATCTATTGATTTCTTCCTAATTCAATTATCCGCTTACCCAGGTGAACTTCAGGGCGACCAGCTTGAAGCCCTTCTGCTCGAAGCGCTCGATGATCTTGCCGACGAGCCCGCGCTGGACGCCATCGGGCTTGACCATGATGAAAGTCCTCTCCTTGTTAGCCGCCATTGTCGCCGAGATTACAGAAAAGAATGCCAAAATTGTGCCGAGCATCAGGAGCTTCATGTGTTCGCCGTTGCAAAAAAATCCCGAATACGAAATGCCGGCAAGGTGAATTGTGGGAAATCGTCAAGTGGTCACACTGGGCAGCCAGCCGCGTTCTTGAGTTTTTGTTTTTGTTGGGCGATTTAAAGGATAGGAAAATACTTGTTATTTAAATTCCAAAGCGGAAATTTGAGTGCATTGCTAAAGTATTCATATTTTTTTCAGTTTATAACCGCTCAAATGGGGCTGCCATGTCGCCTTGTACTTTGAATTTCAGAATGTATTCTGAATGCATTTCATCGAATAATTTAAAGCAAAAATAACGAAAACTCAATAGTATTATTTTTAATTACTAGACACATTAAAACATTTAAAAAAAAACAAAGAGTTGCCCACAGTTGATGATAAAATGCCCGTCAAACTAGGATAGTTACTAGGCACTCATTAGCATCAGCATTCCCACCAGTTGATTCATCCATCCAGCAGGTTAATCAAAAACCACAGGGAAGTCACGTTCAAATTTCTTTTTATGTCTCGATAAGATTAGGTCCATGTGATCACAGAAGTGACAAAACAATACAAGTTTACGACTAATATATACACAGCTGGTAGATGCAAATGTTCGGCGAAAATCACTTGGTCGCGTCTAGTTTGGCTAGGCCGTCGGCATATTGGAACTCCGGACTGTTCTCGTACTCGCACATGTCCAGGGCGATGACGCAGCTCTCCTTGACCACGCGCTTGTCGTCCTCGGCGTAGCGGTTCAAGATCTGAATGCAGTCCTCTGTGGCGATGGCGCCAAGGGCCTCGGCACACTCGTGACGCACCATTTCGTTTTCCAGGCGATCCTCCAGATTCTCTTGAAGGAAGGGAATGCTGCAGGGTTCTTGC
 

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