CR01515

CRIMIC CR01515

Gene Spn55B

CG10913

Information

Donor Location Cytology FBti Stock number
pM37-p02R:18127775..1812778755B7Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence TATGAACGATTAGATATGTGAGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
CTCGTTCTCGGACAACACCA AGGTGCTTGGAAAGGTGTGT TCGTTCATAAATCCTCAATCGGA AGGTGTCCTGGTTTCCGTTC
 
Amplicon 5' Amplicon 3'
CTCGTTCTCGGACAACACCAGGCTGGTCCTGCTCAATGCCCTCCACTTCAAGGGCAGCTGGGCGCACAAGTTCAGCGAGGAGCGCACCGAGGAGGATATCTTCTGGGTGGGCGAGGAGGAGCAGGTTAAAATCAACTACATGAACCAGAAGGCCAAGTTCAACTATGGCTTCTTCGAAGATCTGGGCTGCACTGCCCTGGAGATGCCCTACCAGGACTCTGACCTCTCCATGTTCGTGCTGCTGCCGCAGGAGCGGACAGGTATTTACGCCCTAGCCGAGAAGCTCAAGACTGTGAACCTGGTGGATTTGGCCGACAAGTTGACCGTGGAAGAGGTCCATGTCAAGTTCCCCAAGTTTAAGGTGGACTACTCCCTGGAATTGGCCGAGAAACTGAAGCAGGTACATATTATTTTTTTTTTTTGGGGGTGGGGGGGGTTGATTGTTCCACCCAATTTATGCCACAATTAAGCTCCATTTGGGGTCAGCTTTTGTTTTTATATAACACAGTTCCTTGACCTCCATTTGATGTTGCGTGCTTAAGGTGAAGTTTGGAGTTATTAATAGCAGTAGCTCAATAAATTTCTCCATAAAATCCAGAATTCAATTTTATGGACACGTTGCAGTGCTGCAAACCGCCCACAAAAATATTTATTGCCCACTTATCTCTAAAATTCCGTCTATTGATGTACATATGTATTATTTTTAGTAGCTTATCGACGGTAGGGTAGAAATGTCTTAGATAACCAAAATGAGCTGTTGTGGTATTGCGGTGGCAGCTAGTGGCTCGAACTCAAGATCATGTATCAGCCGCACGATCCTAAAAAAAAATGATGGAATTTTAGCTGCAGTCGGATGTGACACACCTTTCCAAGCACCT TCGTTCATAAATCCTCAATCGGATATGACCTCATTAGTTATTTACTTGGCACTTATGTATTTACCTTTTCACTTTCAGCTGGGCATCACGAAAATGTTTACGGACCAAGCTGAGTTTAGCAATCTGCTGGAATCCCCCGAGGGCGTCTTTGTGTCCAAGGTGCTGCACAAGGCCACCATCGAGGTGAATGAGGAGGGCACGGAGGCGGCGGCTGCCACCGGCATGATCATGATGACCCGCATGATGACCTTCCCGCTGCAGTTCCAGGCGGACCGGCCCTTCCTCTACGTCATCTGGAATAAGAAGAACATCCTCTTCGCTGGCGCCTTCGTTAAGGCTGCCTAAAAGCCCATTTCTCACTTCTTTCGTATTGTTTGTATTGTTGTTGGAAAAGTATTTTTAAATAAATAAGCGCTCCATGATTAAATTACCCGTGTTTATCGTATTGCCAATTGGAAATTCTTGGCAGATGTAAACAAATTTGAACTAAACACCTGTTTCAATAGAAAAATATGAGAACGTTGTTTGAGGATCAACATTTCGGTTATTTAAATATATGTTTTCATGATATTTGAAGTGCGCGCCACTCATTCAGCCTTGCCACCAAGTTCGTGGTCTGTTATCGATTCGATATCGAAAAGAAGTCGCGAAATGGCCTAAAACTGGCCGAACCGCAAGACCAGCCGAATTGAGTCTGCGCAGAGCGCGTAAAACCAAAACGAAGAGAACGCAGGAGAGTCGAACGGTAAATCGGTCGCAGCAACATAAACTTTTAGAATCGCCAGCGAGAGCCGTCTTAAAAGTGCGAATACAAACAGAAGGAAAAGCGGCCGCCTATGGCAACCTGACTAGCAAAACATTCGAAAGCAATTGGCCAAAGTTAGGACGAAATGAGCCGAAAGAACGGAAACCAGGACACCT
 

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