CR01506

CRIMIC CR01506

Gene Hil

CG30147

Information

Donor Location Cytology FBti Stock number
pM37-p02R:20612829..2061285057A7Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence ACTAGCCTACATAACCACGGCGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GCCATCAGACACCTGTTCGA TGGTTATGTAGGCTAGTGTGCT AAGCCCAGGAACATTGCACA ATTGCCACCTGCTGTGTGTA
 
Amplicon 5' Amplicon 3'
GCCATCAGACACCTGTTCGAAATTTGTTAAATTGAGCGTTGCAATAAAAATAAAAATATTTGCTGTTGTCTCTGCCCGCGGTGGTGCTACTTGGCGGATTGGTGAGCTGGTGAGCCGGTGGAGCTGGGATGTGGTGCTGGTGTGCTGGTCAGTCGCGTGTGGCATTCTCGAAAGTGAAAGAAAAATCCGTTTTCGGCATTTGCAATGGCAAAGAAAAGCAAAACGTGTGCGATTTGAATTTCGTTTTTACGTAAAATGCGATTATAATGCTTTCACCCGAGCATCAGGCGGATAAGAGATTACCATTCCAGTCGAGGCAAGTGCCAAGTCATGGATAAAGATGCGAGCACCGATAAACGCCAGCAGCTTTTGGCCAACCAGTCGGGCTTATAATTTATAATCTGGCCGAGAGATCATCCCATCACATGGCGCGCCACCACTAACTAGATCAATTTGGGTATCACATTTATCCGTCTTCAATTTTCCACTTTGCAAGCCAATTTCAAATTTCTGAACCGTTCAAAAAAAAAAAAAAAAAAAATATACCAAAAGACTAAAGTACTACAGGTTTTTTAGCAGATGTTCAATAATTCAATGTTCAATAATTTATATGGCAAAGTAAAGCACGGATATCTTTTTATATTGCCTAGACAGATCTGATTCCTTATCGCATCTTAGTAAAGTTATAAGTAAACTATTCATATTTATCTTTGTTAAAGTCGGGAATTTTGTGTAATAGTAGTACCGACAAAACTGTATCTTCAGACTTGGCCACTTGAGCAATCGCACAGCAAGCATTACACACAGCCAAGTGTAAATAGCACACTAGCCTACATAACCA AAGCCCAGGAACATTGCACAACCGCTGAAGCTTGGAGAGAATGGGAGATACACACTTGACCAGCTGGCCCCGCTAGCCACTTAAAATTTCATTAGATGTTCTGCACTAAATGTGCTCTTAATTTGTGGCCACTGTGGCTCCGCATCTCGGGAGATGGATGGATCACTTCTTATGATTATTGTACAATAATTTAAGAACAGAAGGATATTGCTTACCAGGTAAAAATCAATCGGCTTCTCCCGCGCCTTGTTGTAAGCCTTTTGTATCTCCGTTTGTTTGAGGGCGTGCGCGATGTGGAGGGCACTGGCGTCGAATCTTCCGTACTTGTAGTCAGAGTCATTCTGCGATGGAGAACTGATCTCCCGGCTACCGTAGCCATTCGGCGTGGACTGCCGCGAGCCACCCAGTGGGCCACCTGCACAGATCCGGTAAAACAGTGTTGTTAGCCAAGTTGAGAGCACATGACAGCCCCATACTCACCATCCACTTCGCTCCGCGTGCCTCGGATCTGCTCGTTCACGAACTTGTTGGTGCGCGGCGCATTCAGGGCCTGGCGAATACCCACAGAGGTCTGGTCGAGATGGCCGGGCCCGCTCTTTGGTACATGTGAACTGCAATAGACCTAGAGCGAAGGTGTTTCCGAAGATTATTAGTTGTTGCACATATCGCTGGGTCATATGATATAGTGCATACTTATTCAAAAAGATAAACTTCCAACTAACTAAACTGCATTCATTAAAAGATTTAATCCTCATTACTCATTTCATTATACATCTAGAAAAAAAGGAATGCTCTTTCTGAATCATGTCATCTAATTGCTATTTTGAAGCCATGAGATTTCTTAATGGGCACCGATAATTATCTTCAATTAAAATTTCCTTAAACAAAAAGGAATTTTCAACTAAAACACTTTTGGAATGCATATATCTAGCGTCCAAATCGAAACACACCATTAGCCTTATTGGGTTTCGCTTTGAGTGCGTGAGCAGACCCCAAAAGTCAGTCGCTTGCAAATGCAATTTTAACCACTTCACCTACACACAGCAGGTGGCAAT
 

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