CR01500

CRIMIC CR01500

Gene mdy

CG31991

Information

Donor Location Cytology FBti Stock number
pM37-p02L:..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence CAGCGTCTGCTGCAAATAACCGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
TGTCCTCAAAGAACTCCGGC CTGAGCGCCGATCCCAAT CGGCCAGCAGCTGTAAATTT CTCATAGCAGAGAGTGGGCG
 
Amplicon 5' Amplicon 3'
TGTCCTCAAAGAACTCCGGCAAATCTTGGGAGTTGAGCGAGAAAAACACCTTGTTCACAAGGACCAATGAACCGTAGATGACCTTCAGGGCGCCGGCATTGTTCTCGTGCACCTTAGTCAACTGCATTGTGGCCTGGAGCAGGTCTGTCAGAGGTTTAGCCATACGATCCAGTACAAATTTGATCTCCTCCCACAAAGCCTGCGACTTGAACTCATAACGATAGCGCTTGAAAAGCGAGTGTGCGGTTTGCAAAACGCCGTTGATGACATTGAAATCACCAGAGGCAAATCTCTCCACCATCTCGTCGATAAGCTGCGGCCATTTTTTGGGAAAATCATATTTGCCTATGATGCTGACCGCGTCGCTTAGTTGTTTTTGCAATGCCACCGGTGAGTGGAGCATTAGCGTAACGATCAAGGTCTTAATTGTATTCCTGTCACTTTCGTGGATGCGATCCGGTCCGTCGCTGTCAAGGTGGGCTGCCCAATTCCGCTTAATATAGTTCTTAAAGGCAATGGCTCCCGCCACCCGCGTAGTCATGTCCATTTGGGCTTTGTCTATCAGATTGAGCAGTAGGATCGGGTAATTCTGCTGTAGTTCCGTGGACTCCAAAAGTTTTTCGGCTATAAAAGTAGAGAAGGAAATGGTGTGTTTGTTAGAACTATTGTACTTATGTAAATCGGATGTTGCAAATACAAATATATAAGGAACCGATAGGAGCTTACAGTGTTTCTCGTTAGACTAAACATATTTTCAATTCAAGCAATAAGTTTTTTATACAATATTTTCAAAAGCTTGGTTATGGTGGTGCGGAGTCTATAGGCTTGGTATGCCTTAAATAATCCTAAAACTAGGTCTATGGTTTTGAAAGGTGGCTTGGCAACAGTCATATCAAGATCAGCTAATCTCGATTCAATTAATAGAAAGCGTGACTGTATAAATTCTTTGACATAGGGAAAACTTGTAGACAACTTCAAATGGATTTAAAAACCGTATTTTTCTTTGGCGTCGCCAAAAACCATTTCCCGCGCACATACACATCAGCACCCCTGCACGCATGTGCAGTTGTGTGTGTGCACATGCATTCCTACACACACACACTCACCCGGCCGGCGGACATTGGGATCGGCGCTCAG CGGCCAGCAGCTGTAAATTTGCCTCTGTCACTTCCATTTTGAGTATATTTTCTTTTCGATGGTCCTATTTGCTTTAATTTATATCACTCGTGGCTTGTTCCTCCACACTCTTGCAACATGAAATGAAATCGCAATTCGTTTTCGCACACAATGCTCGCCGATCTGGGGATGGAACTCAGGGCGAGTTTTCGATATCGATTGTTTATAGTTACTGTTACTAGGATTCGATGGTTTTAGATTTTAAATGTTCATATCAGAAATATCGCATATTGAACTTTACTAATTTAGTATCATTTGATATGATATCAATTCCTGTTTTTTTTTTTCAAAAACCAACTGAATGAATTAAAAATTAAAACAAATTTGTAATTCTTTTCCAGGAAATAATTGCAGAGGGCTTGGGCTTGTTCATCCAGATAGTGAACATTGTTGTCTTGGTTTGCCTACCGGTGGTAACAATTCACCTAAAAGGACATGCTTTTAGTTTGAGTGAGAAATTAACTTAGTTTAGGTGTATTTTAAAATATTTAATTATCTGTTAATCTTTAGTGGGCGCTTCAACAGTTTGCTTCTTTTACTCTGTGTTGTTCCTAAAACTATGGTCCTATGTGCAGACGAATATGTGGTGCCGTCAGACTTATTATCAAAAGAATCCGCGGGAGCGTCGACCAAGCATAACTTTGGCGGAACTAAGTATGTTGAGATCTCTCAACGGAATGTCATATATTAAAAATTCATCTTTCATCCAGAAAAAGGAGTTTTGAATGGAGGTGAAGAAGACGAGGACGTTTCCAAGCTGGTGCAATATCCTGATAATCTCACATACAAGGATCTCCTGTACTTCCTTTGCGCGCCCACTCTCTGCTATGAG
 

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