CR01498

CRIMIC CR01498

Gene lace

CG4162

Information

Donor Location Cytology FBti Stock number
pM37-p02L:..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
short band OK

Images

Construct Design Sequences

sgRNA sequence ATAATGACTTCCATATGTTGGGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GGAGTACGACCCCAAAGCAA TTGGGGATCCATGGAGTCCA ATCTGGATCCCACACCATGC GGCTTGGACTCCTGATCCAG
 
Amplicon 5' Amplicon 3'
GGAGTACGACCCCAAAGCAAACACACACATAAGTACGTAGTTTGGGTGCACAATGTGTGTGGGGAGAGCGGCACAATGCTGGTCTGGTCATAATATTGTCATGCCACGCCATGCCCGGCTCGCCTTATCAGGCCCATTCCACCAGTCTTTTACGTAAAGCCTCGCAGAATGCGATTCGCTGGTGAATCACAATGCTCCGCAATCTCCGAAGGCAGCGGTTTTGGGTGGGCGTTGATTTGTCGGTGATAAGAGTTTTGTTTTAAAATTCATGGACTCTCCCTGCGTCTGGGCTGCCAAAGAATAACTGTTGGCGGGTATACTTAACCGATTATCATATTTTTTATTATTCATAACTTTTTAAAAAGATAAGATAAACATATTATAACATTACATTCATTGGGTAATTCCGATACAATTCGATCTTTGGGGAGTGAGCAATTGTTAGTTTTCTTTTAAGTACCTAAAGATTTCATTATTGGTAGCTTTGTATTTCTTTTTTTCGGAAAGTAATACAATGGTTTACATAAAATTTATTTAATGAAATAGTTCCGTACCATGGCGACAACTTATTGAAATCATTCAGCTTGATTTAATGGCAACTAAAGACCTTTTCAAATTTTTAATCACACGAAAATAATGTTAGCGCGTGCAAGTCTCATATCTCCAATATCAACGGATAGAAAACTTTTATCAGCTCGGCAAGATTACAGTATGCATGATTTCAAAGGTAAAGGCTTAAATAAAAAAGAAATACTTAAGAATATGAAATTGCCAAAGCAATTATCTACTGACTCACAGCTAATACTTCTTGGAATCCCCACAAAATCGGTTAGAACTTGGCGTGCGCTGGTCGATTGTCAGTCGTTATCTTGGACTCCATGGATCCCCAA ATCTGGATCCCACACCATGCTCCCTACTTAGCTGTCCTTCTGTACGCGACCCATAGCGTAATTAGTATTACACTTTTTCTATTTATTTATTTATGTCGGTTTCGATTTTGATCTTTCTACGGTAGCTTCGAGCTCGTCTATATACCCTAACACTGGGACTCCATTAAATAACTAGGCATCGCGTAACCCACTTGACTTTTCGCTCCCCGCACTCCGTCTCATCGTATTTAGTAAACAAGCCCGGTGGCAAATGATAATAAACACGAAATATTATTGTCGCCGACTCGTTATTGTTATACGATTAAGCTGACGTTCAACTATATATATTTTTCGGAATATATATCTGCAGTTTGTGTTGCTCAGTTAATGAGATATATATATGTCTCGTATCGGATCCTTTGGCCAGCAAAATCAATAAGCAAATGATATATACACTTAAATAAGTACGTTAATTATAACGCTCTATTTGTTAGCTGGTGAGGTTGAAGTGTGCCAAGTTGGGCACACAAATACTATATACATTTATGGCTTCCCCTGTATGATTTTTGTGTCTGTCTCTTTTTGTGTGACTTCATGTCGAACTCGGCTCATGTGAGCGGTGGAGCAAATTGTTCAGCAGTTATCGTGTGATTGTATTTATATATGTACATACGTACCTTGCATATATGGATCTTTTAGTTAAATGTTAATTGACTGATGAATTGACTGAACGCATAGAAAGGGCAAGCAAGTGAGAATGTAGAATTTTTCATTTTTTTATAAAAGCGTTTACGCATGAGAACTATAAGTTTTGATATACATACTCGTATAAATGGGTATTACTGTGCTGATCCCTTTGAAGCGAACATGTTTACAATCTGTCTTACAATTTCCCATCTAGCCCGTCTAACAATATTGATTATTTGCTCTTTGATGAGTGATTTTCAATGGAAATTATGAAGATTCACAGTGATACCAATGATAGCGATAACCAGCCAATAATCGTTCCTCTCCCCTCTTACAGAGTCCCAGCTCGATCAACTACCATCCGGAAAAGAAGTCGATCCCCAATGGGCATGCCTCCATTCTGGATCAGGAGTCCAAGCC
 

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