CR01482

CRIMIC CR01482

Gene CG10188

CG10188

Information

Donor Location Cytology FBti Stock number
pM37-p12L:19507402..1950742137E5Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence ATATTTGTATACCTGCAACATGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GGCAGCAATCCTCTCGAAGT CATGGCTGGCCCTGTTGATT CTGAACTACTATTGCATTGCACA AACAAGGTGTTTTCGGGGGT
 
Amplicon 5' Amplicon 3'
GGCAGCAATCCTCTCGAAGTCACGGGCCAGCTCCGCCTCGTTTAGCGAATGGGTCGAAATACCTGCGAGTAGTCAAAGTTAGAGAGTCGGTCCGTTAGCAATAACTAATCGCAAGGGGTTTCTGATAGCTTTGGTCGGAGCTACCAATACCAAAACACTATATGGGCTTACAGATAAGGGGGTTTTGTGGAATTCCACATCGAAACGAAGCTAACTTGGTAGTAGCCCCTACTCTTTGGTTGAGTGGCATATTATTTTGAGCAATCAATTGGCAAAATTTATTGACTTTATTAACGATATCTTATCATACATATTTTCCCATTTTTTTTCCGCGAGTCCCACGGTCACACCTCTACAATATTCTACATTCTACAATAATTTAAGATTAGGGCTTGTAGTTTCTAGTGAATCTGCTTGGAACAGAGAAGTTATTCTGTCGAAAGGTCATAACTTTTCACTTGGAGCCATTTAAGTTTAGTACGTTACCACGGCACCATATTTGAAAGCTATTGAAATCGGATTGTAAAGCTAAATGGGAAGAATTTTCATTATACTGGAGGCATAATTTACCATACGATGCGTACATAAGTACAAGCGAATGTTTCATTGCTCACTAGGAGAATACGAGATAGAAAATTTTCAAAGAGTACCCGGATGCGTCCTTTAAGTTCAGATAACTTAAAATCCTTTTTAGAAGATCAACAGATAAACCTAATAAATCAAGTTTGCTTACTAGAAGAGAATTATTTATAAAGACAAATGCTTTACTAAAGTCAGTGTAGATGACACCTGTTTGAATATTATTTCTGAAGCCCTGTATTACGAAGGAAGTTAGCTCCAAAAGGTTATTGGTTGTTTATCTGCGTTTCTGCTGACATGGTGATATAATTGACCTACAAAGGCGCTGCAAATGAGTAGTGATAACATTTTCAAAAACCTTTGGAATAGCCGACAATTTAGAGATTCCTCTGTAAAATCCATTTCCGACTTAATTTTGAATGCATTTTTGTTTAGTATTATTTTAAACTTTCAACACCTAAACACTTTTGTCTAAATAATTAAATAATATTGGCAAATTTACCTAATCAACAGGGCCAGCCATG CTGAACTACTATTGCATTGCACACTTATGGCTTTGCCAACCCACCCCCGAAACCTTAGTGCATATCTACCTCCAGTGCTGTTCCTGGCCGACCTTCGTCGCTGCTCGGTGACGCGAATGGTTTCCTGCTCCTCGCTGTCCAGGCTGGATAGGCTGACGCTCTTGTTGGTGCAGTCGCCAGCGCCGCTGCCATCGTCCACGGCTGTCCAGGATTTCCGGCGGTCTGTGTGAAGAAACTGGCACTGCTGCGGTGCCGTGGAAGACCACATGGCGTAGTTGGAGCCACCGGACGCGGGACCCGTGCCCGTCCCCGTAAGATCGGGCAGCGAAGTACTCAGTATAGCTGCGCTGAGCAGTCCATGATTGGCCAAGCTGCCGTCCTGCATGTGCTGGCTAGGCGAGGAGGAGTTCTTCATCTGGACGACCGTTTCGCGGATGTTCTGCAGCTGGCTCAGCGTGTCCTCGAATATGTTGTTGTACTTGGAGGCCAGGGCGGGACTGTGTGGCGTCACATTTATGGTGGGTATCATGTTGTTGTTATTGATGGTGTTGCTGGCAGGATTAGATGCTGGTGGCGGAGCAATGGGGGCGCGCGCTGGACCAGGAACGGGTGCGTTTATTACGGGTATGCAATAGTTCTGGCTTTCGATGGAGGCGCCTCGGTTGGTAGAGCCACCATTAACCAGCGCTTCCGAGGACTCGCTGCAGTTGGAGTTCAGAAAATCGGTGACCACATCATTGTCGCTGTCCTCGCCCGCGTTGTCCATAATAGGGTCTACTGAATTCTCTGCGTTTTGGGCCAATTTCCTTTGTTTAGCGACTGTGTTCTATTTTACAGCTGGCTGCCTAGAAAAAGAAAGTTTCAGTATACAACGCACACACACTCAACCGCGACGGGCACCATACATGCATATATGTGTGTACATACGTACATATACACTTGTGTATGCACTTGCTTTTTGCTATTTATTGATTTTCCCGCCACCCCCGAAAACACCTTGTT
 

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