CR01469

CRIMIC CR01469

Gene SmydA-1

CG8503

Information

Donor Location Cytology FBti Stock number
pM37-p22R:14244694..1424470650E5Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence TGGTCATAAGTATTTATGATTGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
ATTGGTCCACTCAAGAGCGG TGACCACAGACAAAAACCAGA GATTGGCGGGCTATCAAAATT CCGGGATCACCATTAGCTCC
 
Amplicon 5' Amplicon 3'
ATTGGTCCACTCAAGAGCGGTGGTCTATAAATAGTTATAAGAGGACCGAGCGTAGAGCACGGCAGCAGATGTGATGTGTGTGCTCTGTTCATCAGCTGGCCCAGTTTATAGGAATTGAGTGTGGCGTGCGGAGGTGGATGCACATCGCGCAACTGAATGAGAGTGCGGACGAGATTCTCGCCGACTGAAAACCATCTCTTTTTGGATTGTACATCTGCCGTGCCACCATGAATCCCTGCCATGTGTGCGAGGAGCCGACGAAGAACAAGTGCTCCAACTGTAACCAGGTCTCCTACTGCAGTGTCCAGCACCAGAAGCAGGACTGGAAAGTGCACAAGCCAAGCTGTCATCCATTCAAGGTACTGCGGTACTGATATCCAAACGAAAGGGCCCAACATAACCTCAACCCTCTTACAGATCGCCCACAATGAGCAGCTGGGCCGGCATCTGGTGGCCACCCGCACCATTAAACCATACGAGATAGTCCTGAAGGAGGCGCCTCTGGTCCGGGGACCAGCACAGATCTCCGCACCCGTTTGCCTGGGCTGTTTGAACGGCATCGAGGCGGAGGATCACATCGAGTGCGAGCAGTGCGGTTGGCCGCTGTGCGGACCGGAGTGCAAGTCCCTGGATGAGCACAAGGCGGAGTGCGGTCTGACCAAGGATCGGGGTCAGAAGGTTAATGTGCAGGAGTTCGGTGGACCACATCCCCTGTACACGTGTTTGAGCACCGTAAGGTGCCTGCTGATTGGCGAAACAAGTACGGAGAAGGCCAGCAAGTTCCAGGACCTGGAGTCACTGGAGTCCACCAGAAGGGGGTCCAACCAATGGAAGGCGGATCTGGTCAGCATCGGGCAGTTTATTCCAAAGTGGGTACACAAACATTTATATATCATAATTGAATGAATCTATATAAACATAAACAATCTTATCTTATCTCTCTCACCGATATAAGTACGTTAGTCTGGTTTTTGTCTGTGGTCA GATTGGCGGGCTATCAAAATTATAATCTAATAACATAAAATTATTTCAGCAAGTTAAACAATATTTCATTTTCTGTTCTTTGAGACAGTTACATGCTTACAAGCGTATTTTCTTTAACGAAATATAATACTTTCAGGTTCTTTAAAACACAGAAGTTCACCGAGGAGGAGATTATGAAAGCAGTTGGTGCTCTGCAAATAAATGGACACGAGGTGCCCACCACTGATCCATCTCATGTAGCGGTCTTTTATACCGCCTCCTTTACGGAGAACTCCTGCCTGCCCAATCTGGCCAAGAGCTTCAACAAGAACGGACACTGCATCCTCTGGGCACCGCGCGAGATCAAGAAGAACGCCCACCTGAGCATCTGCTACTCGGACGCCATGTGGGGCACCGCCGATCGCCAGCGCCACCTGATGCAGACAAAGCTGTTCAAGTGTGCCTGCGAGAGATGTGTGGATGTCACCGAGTTGGACACCAACTACAGCGCTATTAAGTGCGAGGATCGCCAGTGCGGCGGATTGATGCTGCCCACCAAGGCGGACGAATGGAACGGCAGTTGGCGGTGAGTCTCCATGTGCCTTCAACCAAGTCGTCGTGAAATGTATGTGATTTCCTAATCTCTTCTAGCTGTCGCGAGTGCCACAAGCAGGTCCAGAAACACTATGTGGAACGCATTTTGGAACGGGCTGGCAAAGACATTCAGAGCATGGAGAAAATCGCTGAAAATGGATTAAAGTAAGTAAGCATAAATATGAGAAAAGGTCTCCTAAATGCAAATCTTTAACTCACAGATACCTCAAGCATTACGAAAAGTGGCTGCCGCCACAGCATTTTCACATGAGTGAGATTAAAATCCTGCTCGTTCAGCTGCTGGCCAAGGATCAGAAGGAGCTAATGGTGATCCCGG
 

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