CR01370

CRIMIC CR01370

Gene JhI-26

CG3767

Information

Donor Location Cytology FBti Stock number
pM37-p02R:16289742..1628975753B1FBti020692083292

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK CTGAGATCCTTCTTATCCCGCTTCAC ATGGATAAGCTGTACGGCAGGAAACG

Images

Construct Design Sequences

sgRNA sequence AGTTTGCTGGAAAACAAAACTGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
ACTCGCTTGCATTGGACAGA AGTAGTACGCAGTTAAGGCACT TCCAGCAAACTCAGATAGCTT CCTTGGCGTGCTCCCTATAG
 
Amplicon 5' Amplicon 3'
ACTCGCTTGCATTGGACAGACGACATCGTCGTGTTGAGCATTCTTCGACATTTTCAACTGATCGTGTCGGCCGCTCGTTATTTTGCCTTTTCGCGAAGAAGAGGACATATATGACATGTTATCCAAATCCGTGATCCTGATCGAAGCACTTGTCCTCTTTGGCACTTTGGATGGCATGCTGATATCTCGACTTTGCTGCGATCCATTCGTGAACTTTCGAACGTAGGTGTTGGCCCTGGTCATTGACCCGGTCGTTTTGAGTAAATACCAGTTGAGGCCCATTGGTAGTTGGTCGATAGTCGAACTGCAACGGTAGGATAGCTTTTTCTAAAACTGGAAATTTTGACTAAAAATATATCGATACCTGTGATTATGGAAAATAGAACTTAATCCAAAGGAATCATTAAACTTTGCACACAATAAACAAAAAAAAAAAACTAATTAAAACTAATAAATAGCGTATCCCCCTATCTTTGCGTCATGATATCTTATCATATCATTTTAACATGCCAAATTGCATCATGCTAATCATTAGGTAGGTACTGCTTCTCTATAAACCATAAAGTGCTTTTAATGATTAGAAAGCTTTTCATTTCCAATTCGCTCTTATCGTGTAATCTCTAACAACGAATGAGCTTTTGGAGAGTAGTACAAAATGAGCTATATGAAATGGTTTATGCTCATTCGCTATCCACAAGCGAAGCGGTTGGGATCGTCAGTACCGACGAAATGGAGAAGATCGAGGCTGAGAGGCAATGGCTGAGATACACGGTTCTTCCGTCAATTCTGAGGAACGGGCGCCTGGTGGACAACTACAGCGAGTCCAAGGTGACCACATTCCATGTGGGGGACATCGACATTGATGTAATTGGCCACTCGGAGGCCTTTATGCTAACTTTTTGCTACCGGACAACCATTAATTTTGAATACGACGGGCAAAAGTTTCAGCGAAAAATGGTGGTTAAGGTAGGTTTATTCCATTCACATAGTATAAATAAATCTTAAACAATCTATCTGGATATCTAAAAACACAACGAAAAGATTTACTATACTCGCGGAATTTAAATTATATTATATAAGTGCCTTAACTGCGTACTACT TCCAGCAAACTCAGATAGCTTAGTGAGAGTGTGGGTTTTATGGCAAACATTGTGAAATAGAGGGAGGCATCTCAACAAAAGAACAAAGCGAATTGATTTTGAATTCAAAATAAATCACATCTAATGATGAAGTTTACTTTGGCATGCAATGACCCCAGAATACCAATAATTAATTGTTTTTTTTTTTTTTTGTAGAAAACACCTGCTATGCCACCAGAAATGTATGAATCCATACAGTTTGGGCCCCTTTTTACCAACGAGATAAATTTCTATACGGAAATTCTGCCAGAGTTTCAGAAGTTCACCGATGGCAAGTTTGCCGCACCCAAGTACTATTACGGCGAGTTGAACCAGCATTCGGCTGTGGCCATTCTGGAGAATTTCGCGGAGCAGGGATGGCGGGTCACCAAGGATCGTGTGGGTCTGAGTCTGCAGCACGCCATGATTGCCGTGAGTTACCTGGGAAGGTTCCATGGCTTTGCGTACGCCATGAAGCACAAGAACCCGGAAAAGTTTGCCCAGCTGACTGACAATCTGAAGGAATCTCGCTATGCCAACGATAACATTCATCCGGAATGGAAGCTCACCATGAAAACGAGCATCGATCGAGCGGCCAAGGCGGTGGCCACCTATCAGCCACAGATCGACGAGGAGTTTGTTAAGAAGTTCTGTTTCATGATCTCGGACTATTCGCAATACGGTAGGCAACGGGTGGCGCCCAGGGAGCCACTGGCCACCCTGTGCCACGGCGATTATGTGAGGAATAATGTGGCGTACAGGTACGATGACAAGGAGGAGCCGCAGGAGATCATGATGTTCGACTACCAGACATTGCGAGTCTCCTCTCCCATGGTCGATCTCAACGTCTTTCTAGCCGTGTCCATTTTTGCCGAGGTACGCGATCCAAACTTCGAGGCCATCTTCTGTGAATACACCTTGGCGCTTCATAATAGCTATAGGGAGCACGCCAAGG
 

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