CR01369

CRIMIC CR01369

Gene dmpd

CG11866

Information

Donor Location Cytology FBti Stock number
pM37-p02R:10118622..1011864846F1FBti020691983291

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK GCGACCTATTAGCGGAAAACGTTTCG TGGGCAGCGTATAGAAGTCCTTGTTG

Images

Construct Design Sequences

sgRNA sequence AGTGCAAGCGTGGTACGACTGGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
TTTGAAATGGCTTGCTGGGC GCTTGCACTTGGGCAAATTG ACAAACTACCTGCACTTGTATGC TGAGAATCGCCTGGACTTCG
 
Amplicon 5' Amplicon 3'
TTTGAAATGGCTTGCTGGGCGCAATTGGAACAGCAATGTTTCAGTGGAATCAGCCTATAATAATCGATGTTTCTGCAGCAACTTCACTGCTTGCAAGCTATCGGCGCAATCGATTGTCGGTGCCATCGATGCGCTTCCAGCCCTAGACACTTTGACTTTTTTTGCCACGGATTGTGTATGTAGTTTTAATTGTGCTCGAAAAACTGCGTTAAAGTGGATGTCAGCGTGGATGAACTGCGTCTCAAAGAACTAGACATATTGGACCACGGCGAAGCGCGTCCAAAAACAATGCAATCGCAATAGTTCCATTTACTAAAACGGGTACGAAGCCATCTCGAAATCGAGTCGCAAAGTGCGTAGCACAAAATTTCAAAGAAATTTCGAAAGGGGGGAGAAAAAGCAGCATAGAGACGCTACATATATATGTATATAGTCGAATTAATGCGTATATACGTTGTTTTCTGTGTGTGTTCCGCTATTGCAGCAACTTCAAAACGCTGCTGCAAACGAATTCTGTCCATTTAGCACCGAAACGCCACTGGAAGCAGAAGGAAATGGTGTCCACCCGCCAAATGTGCAGTGGAGGCGGTACGTCCGCTTCGGAGGGGGTTTCGCCGTTCGGGGGCGCGGGTTCGGGATCGGCCTCTGGAACGGGAATGGCTTCCGGATCAGCAGTGTCATCCGGATCGGGTACTGGAGTACAAGAGCCCCCCGAGTCCACAGCAAACCGTTCCTCCGTCCTGCGGCGAACCACCAGCTATCAGGGTAACCAGGCCCACAGTTCTTATCGTGTCGTCTCGGCGGGCAGCCTGGCCGAGTCCGCCAGTGCGGTGACTCGCCTCCACTCGTACACCATGCAGATGCAACGGCAGCGTCCACCGACGGCCACCACCACCACCACCACCAGCGCACCCAGTCTGTACGATCTTCCCAACGAGCTGATTGAGAAGATCCTCAGCTACGTGGACTACAAGAAAGTTTCAAATCTCAGATTGGTAAGTGCACTGCTGCCCCTCTGTTCCACCCAAAGCGCTGGCTATTAAATCAATAAAAAGCAATTTGCCCAAGTGCAAGC ACAAACTACCTGCACTTGTATGCCTCTTCTGATATGTATAAGGTGTATTCTGTCGCTCTGCTGTTATGAGCATTATTTTGCGCCATGCGTTGCACTTTCCTCAACGGATTTCGGATTTCCCTTGGCAGGAGGCAATTAAAACTTTGTCACGAGGGCACATGCATTTGCGAATGCTTATTTTTAGCAAAGAACTTGGAATCTTGGCTTCGGTTGGGGGTATGAGCAAATGTATTACATTTTTCAGATTTAGCGGCTTTAAAAACACTAGGTAATGGTTGAGTTCATTCACTTTTTTTTTTAATAAACAACTAGCTTTACACAGAAAGCATTAAAGTGAAAAAAGAACAAGCCAGTTGAAGGATTATACACATTCAAATAAAGAGATAAACATTTATGAATATCTAATATTCTACTTTTCAAACAAATTACACCAATTATAGAAATATCACCATTTTAAAGTCACGATTATCGCATACAGACTGCGTAAGAAACAAATCCAAGAACACTTTCACAGAACAGCTTTAGTTCAAGGTTCAGAGGGTAACTACACTTCGTTGTTTGTTTCTCGTTGGAATTTTTTGCCTGCACCCGCGTCTCCCATCCACAAATCGTTGTGTATGTATGCACGGTTATTTGTCGTAGGTAGCGGAGACAAATGTAATCTGATATAATTTAATAACAGGCAGAACCACTAGCTCGTGGATGAATGCGGGCACAGCAGCCGAAAAAAGAAGTCGGAATCTGAGCTTAGCTTAGCCCAAAGCACGACACATTTTTATCTGTTTTACTTACCCCCCTATTAACTTGATTCCCGGATATGCATACTCGCAGGTTTCGCACCGCATGAACGACATTTGCATGGCCATGCTGAACACAGCCTTCACCAAGCAGATCAAGACCACATTGAGCCGCTTCCAGGCGATCAAGGCCAGCATGCCGCGCCGGGAGTCCCATCGTCGTAATCATCCGTTGGCCTGCGAGTGCGACATCATCGAGACATGCTACATGCGTCTATCCCTGCTCCAGATGTCCATGGGCAAGCACATCGAGCGGGGCCATTGTTGCTTCTTTCCGGGCGCCGTAAGTGCAGTGAATAGCATACCCGAACTCGACAAGCGCTAATAAGATGGTTGTTTATTTCCAGATACTAGACGAAGTCCAGGCGATTCTCA
 

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