CR01367

CRIMIC CR01367

Gene pio

CG3541

Information

Donor Location Cytology FBti Stock number
pM37-p02R:24588930..2458895360D8FBti020691883290

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence AATGCGATTAACCTGGATGCGGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
ATCCGGGGTGAGTGGATGTA CCAGGTTAATCGCATTGGCA GACTGCTCCCCTCCTCAGT CCTCAATGAGGCAGCTCCAA
 
Amplicon 5' Amplicon 3'
ATCCGGGGTGAGTGGATGTAGATAGAGTGCTGGTCTTGTGACATCATCACTCCGCTCCCTTGTGTGTGTGTGTGTGTGTGTGGTGAATGAAATGTGTTGGCAGCGTCAGCAGTAACAACAAATCATTATCGCCTCTCGGCCATCAGTGTAAGCCGCATTTTGTTGTCTTGCGACGCCCAAAACACCGATCCACATGCACGGCAAAAAAATGATCTCATTTCCGATATCATCTGGGTGGTGGTAGTATGGTACTATGAAATGGGTTTCTACAAAAGATCTACTTAGTTAAGACAAAGACATAAAATAGTATACATGGAGCACTTCTTTTTGAAACGGTTATTTCAATTTAATAAACCAAATTACATTTTTGATGGCGTGCCGAAGTATATTATCCGGGCTTATAGATGACTCGACTCCGATTGCCACATATTTCTTTCTGTGTAGTGACCACCATGAGGATGTGACTCGCCTGCAAAGTTCTCAGCGATCGGCAGGAAGTGGAAAACCGTTTGTCACGGGCTATTGATATGCGCTTAATATCTCGTAATGCAGCAGCTTCTGTTGCCGAGAAAATGCGGAAACACGAGTCATGATGCCACGCTGCCATGAGTTGCACTTGGTGGCTGTCTGTTTGCCCAGCGCTCGACGGCTGGCACCGCCGAGATGCCGAGATGTGGAGTTCCTCGAATAACCAGCACCACTTGGTGCGAGGCTAACCCATTTGCAATGAGCTAAGCCAGCACGCGAACCCAAATTCGCCGCTGTTGACTTGACCCATTTGGCAGCAGAAAGTTGCCTTTGGGCCACGATATTCATTACAGAATCGCTGTGTTCGAGTGATTTAGCATAATGCTATATGTGAGCAGTGGGTGTGGAACCGGTTTCGGCGGCTTTCAGTTTATATTTTCGGTTTTGACTATTTGCCAAGGCTTAATATCCGTCTTTCGGTCTTCTCTCACTCTTGCAGCTCGCACCATGAAGACAGGCACTCGAATGGACGCTTTCCACACGGCGCTGCACTTAATCGTAAGTATTCTGCACTGGGTTCTCTTCCCCAGGCCCATCGAAATGGAAAATTGCTGGAGTCGGCCAGAACACATCAATCAGGCAGCCCGCTGAATGACAATTGCATGCCAATGCGATTAACCTGG GACTGCTCCCCTCCTCAGTATGCTAAACGCGAATCCGAGTGGCCAAAAGTGGCACACCAGCGCCATTCTAAGCCGCTTGGCTTGGCCCCAAAGATCCGAGATTCAGGTTCGGGCTCGGGTTCAACTCTAACCCAGACTGGCGAAAGTTTGCCAGTCCGTCCAAAGGAGCCTCAGAATCGTGAGAATGCAGCGGTTCCCCTACTCGCCGGCAACACGAAATCGAAATTTCCCCTTTAATTAAAGAAGCTTACTGGCGATGGATTAGGCCAGGGACTTAATTTCGGCTTGTCGTGATTTATGTCAACGCTTTGTGCTTGAAAAGGGGTCGGGCATCCATGGTCCATGGTCGGACCTGATTGGATCGGATAGAGTAGCATATATACCAACTATGCTAATGAGTGACTTGTCTCCACTTTCTTTGCAGACAATCGCAGCTCTGACGACGCACGCGGCGCAGATTCCAACGGCAATGAGTGAGTACATAAATCTGTTCATTAGGAAGCTAAACCGAATTCTATGGATTGGGACACCGAGCCACTGGGGGCCCAAAGAAAAATTGCCAGCCAATTGGTAACGCAGACAGACGCTGGCAAGATTAAACACAGTTGGGCGCTTTGTTAGTCGCTTGAAAAACATAAAATAATATTCAAATGAGCCATCGAAATCGAAGTTATCAACTATTGTAGTTTCCGCTCAAACAGAAGTAAACAGAAGAGAGAATATAATCAAATTGGGAATATTTAGTATAAATTTAAGGTTAAGTAGGAAGAGTATTTAAACACAAAGTACCTAGTTAGAAATCCATCATGACGTAACCAGCAGCTTACAAATATGTATATTTATAAAATGGATAGCAATGCCTGATGAATTGGTTCCCCTTCAAATTGGCGAAGCATTACCTAATTAGTTATAAAGGCACTCCACTTAATCCGCCAGCGTTTTTGCCATTGTTAAGTAAACCCGCTAAGCTGGCTGTTGTCTCTCCGGTTGCCAAATCTCTGTTGACTCTGTTTGCCAAAAACCAAAACGAGGTATTATAATCGCATGGCAAACAGCGGTCCAGTGAAGTCGCTTTGGAGGCTTTGGAGCTGCCTCATTGAGG
 

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