CR01358

CRIMIC CR01358

Gene CG4586

CG4586

Information

Donor Location Cytology FBti Stock number
pM37-p2X:6971043..69710486E4FBti020691383285

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence AAACACATTCAGCTCACCCAGGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
AAGGAGCGCTACGAACAGAC GCTGAATGTGTTTTATGGGCT CCAGGGTTCTTTAGATAATAAT GAGACATTCGGGCTAAGATC
 
Amplicon 5' Amplicon 3'
AAGGAGCGCTACGAACAGACCGTGGCCCGATCCACACGCTTCCTGGCCAAACTGCGCCAATGGCAGCGGGAGAAGCAGCCACAACTTCAGCTTAGTGTGAATATGCTGAGGGACTTTCGGCTGCTACTCTCCGGATCCCTGGGCACGGGCCTCTTCCAGCAGAGCTTCCCCCTTCGGGTGCATTTCTCCATGTTCATGACCACCTTGCTGAGCCAGGGAACCGAGGAGCAGCAGGCCCAGTGGCTCAATCGATCCTGGCACATGGACGGCGTCCTGGGCACCTATGCGCAAACGGAGCTGGGTCACGGCACCTTTGTCCGGGGCCTGGAAACCCGAGCCGACTACGATCCCATCACGCAGGAATTCATCCTGAACACCCCAACGCAAAGTTCATACAAGTGGTGGCCCGGCGGACTTGGTAATACCGCCAATGTGGCCATTGTGCTGGCCCAGCTATATGTGAAGGGCAAGCATTATGGCTTGCAATCATTTGTGGTGAGGATCAGGGACGAGAGGACACACGAACCGTTGACTGGAGTCGATGTGGGCGATATAGGACCTCGCCTGGGTGGCAATGGCGTTAACAACGGCTTTCTGGGACTCCGCGACGTGCGGATACCGCGCAATCAAATGCTGATGAAGAACGCCCAAGTCCTGACCGACGGCACCTTCGTCCAGGGTAGACCTCCGCTGCTGCTCTACGGCACAATGGTCTATGTGCGGGTAATTACCGTGAAGGATGTGCTCTTTGGACTGCTCCAGGCTGCCACCATTGCCACCAGGTGATTATATAGTTTCTGGGGATTATGTTTATCATAGCTGTTATTAAGCCCATAAAACACATTCAGC CCAGGGTTCTTTAGATAATAATTATCATTATCTTTAGGTATTCCGTGGTGCGTCGCCAGAGCCGGATTAACAGCGATCAGCCGGAGGTCTCTGTTTTGGATCACATCACACAGCAGGCAAAGATTCTACCGCAAATTGCTCGAGGAGTGTCCTATCGCCTGGTATCCGATTGGCTGTGGCGCTTCTACGAGGACGTACTCCGCCAACTGGAGGATGAAAGCACGAAAGGACGCAACTCCCTGCCGGAGCTGCACGCCCTGAGCTGCTGCCTCAAGGCGGTGGCCACCGACGAGGCCAGCGAGGGAGTTGATCTGCTGCGCAAGAGTTGCGGTGGCCACGGATTCCTTTCCTCGGCGAATTTCGATAGCATCTATGGTCTGACCGCCGCCACATACACCTACGAGGGAGAGTACACAGTACTCCTGCTCCAGACAGCTCGTTTCCTCGTCCGGCAGTATGCGGATAGCTTAAAACGCAAGGTGCTGCCCTCCAGTGTCTCCTATTTGAGGGATACAGCCCGTTTGATTTGGGGCAAGAATCTGGTTGCGAATTGTGTGCGAGCGCTGGAGATTTCAGCCATGGAGCAGGTGCGTGATGCTTGGCAGACCCAGAAGATGCACCAATCCGGCAAGGTGAGTCCCGAGATGGCCAGCAATCTGGCTGGAAGGCAGCTCACCTCGGCTGCCATAGCCCATGCTCACGCATTCTTCACACGCAATGCACTGGAGCAAGTGGAAGCACTTCGAAAGAAGGGCGATCTTAGCCCGAATGTCTC
 

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