CR01353

CRIMIC CR01353

Gene Gr43a

CG1712

Information

Donor Location Cytology FBti Stock number
pM37-p22R:7392762..739278743B2FBti020690883280

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK (weak) OK

Images

Construct Design Sequences

sgRNA sequence CTACGGGCTTGTCCCATTCAAGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
CACGGTGCTGACCTTTTGTG CACTCGGAGTGGAGTCCTG GGACAAGCCCGTAGAGATTAGT ACACTCCGTCTAGTCTGGCT
 
Amplicon 5' Amplicon 3'
CACGGTGCTGACCTTTTGTGGCTTGATGGCACTGGTTGCATTGTTCTCTGTTGGGTGGTTTACAACTAATCAAAAATCCTGGAAATTTAAATTACCACTTACCTGCAAGAAAGCTGCTCGAAAGCATTCGGTTCAGACGGCCAAAGCGCCTTCCTAGTCCATAGGCCGTGTTGGCAATGTTAACCTGCAAGCCCGTGAGGATGAAGTAAAGACTATAGAATGGAATGTACCAGTGCACATTGAGCTCTGTCGAAGAGAAAATGAAAAGGTGGGGATTCAAAGATAGATTTCTTTCATTTTAAGATTTATTTGGCGGCTTATAAATGAATACATTAAAGCGCTAGCTACCCGTATCCGACTGAGCTATGTTCATATCCTGGGCAATGCGCATCCACGTCCCTACATCCAGGCCGACCAGAATCGAAATGGCCAGCAGAGAGACGGCAGCCATGCCCAAGGCTCGCCATCGATCTCTTCGAAAGTTATTGTAGGCATTTAGGGTATTATCGATCTGGGGTGTTTATTGGGTGATTATAACAAATAAAAGGAGGGTGGATCTATGATTGACGTGATCTCACCTTGTTTAGTCGATCGTTGAGCTCGAGAGTGTCGTTCAGGCTGAAAAGGCCACAGTAAACACCGGATACAATGGCCATGACCACCACAGACACGTCCAGGGCCGTCACCACCTTCGACGTAGCCGACTTCATTCTAAAAGATGCACTTTTGATTTGTTGGTTGGTTTTGTTTGCAGTTTGTCAGAGCAATTAATTGGTCAAGATCGAATGTGTTTAGAAGGGAGCAGAGGTGATGATTAGATAGAGAAAAATCGAGAAAAGTATACACACAATTAGGATCAAAGTAATGACACCCTCCGCAACAAAATCATATCCTTCTTGAGAGGAAAGTATTACTTATACATATTTGATAAGATATGGGTTCTAAAAGTGCAGGACTCCACTCCGAGTG GGACAAGCCCGTAGAGATTAGTAAAAAAAAAACGGCACAGAACTGGGACCTTACAAGTAGGAAACATAGATGTAGATACAAAAGTACCTACCGCACGGGAATTTCGGAGTTAGCATCGAACAGGAGACCCCGATAAGTGAGAAAAACTACGCAGATGCGGATAGCGTTTAGTCAGAAGGAAGTCACCCGCCTTTGGATGCTCAACTCACCCATAACCACCGTTAATGTGGCCGAGTATACAGTGAAGAGCCAGCTGCGACCAATTTCCACCCGGCCCTTGGAGTTCCGGACCGTAGCATATGGAGCCAGGGCCAGCACTTTGCTGATGTAGAAGATGCCGATGCTGGGCTGGGATATCTCCATAGTAATTCAGAACGCGGGACACTCGCAGTACGACCTCTGGACGAGAACTGTTCTCGAGTCGCCAAATACAGGACCTTAAATTGCAAAACGCCATATTCGACACCAAACAAATACTGCGCTCCCATATTTGACTATTATTAGTGGCCACCACCATCCACGCCCCACACCATGACCACATGGCCCTCGCAGACCGTCTGGGCAAACACCCCATGTGCGAGTGAGTAACACATTTGCTTATCAAAAAGCTCGGCTGTCCGCGATGTAAATAATAATTAGCGTGTGAAAATACCAAGCCGGAAAAAGGACACAGGAGCAGGAGCCAATTATTTTGGGGCCGACGTTGACAGCTGCGGCAGCTGCGACAGGTGCACCCGAGTGAATGAGGTCCACAGCCCAATTGTTACACAATTTCTCATTTATTGTTGCGACTCCACTCGGCGGTCCAGCCTAGTGGGGATCGCTATTAAGGAACAGTGGCAGATGCGCAATTAATCAAGTTAATTCTATCTGCCAGCCAGACTAGACGGAGTGT
 

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