CR01350

CRIMIC CR01350

Gene Myo31DF

CG7438

Information

Donor Location Cytology FBti Stock number
pM37-p22L:10498984..1049901431F3FBti020690683278

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence GGTTAATTTACACTCGCTGATGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
AGAGCTCCCGACCCTTGTAT ACTTTCGGAACACTCTGGGT AACCCAGATGAGCTACATAACC CGAGTGTGCTGTTCGTCATG
 
Amplicon 5' Amplicon 3'
AGAGCTCCCGACCCTTGTATTTGCGAATGGTTTCCGGACCGTAGATGTTCATCTGTCTGTAGGGATTCATCGAGACGCACACTTCGCCGATGTATGTGTAGATGGATCCGTTTTGGAATCTAGAGAAAGAGTAATGGACAGTCGTTCATTAGTTCGGCAGTGTTCGCTGCAGTGTTTGGAATGGCATTTCCCCAAGCTTACGAAATACATTTCGAGTGAGTGGGAGTGACAGGTGGGGAGCCACCACCCGTCGTTGGCGCCTGTAGCTTTCAATTCGAAAATTGCTTTTTCAACATTCTACATGCGGGTCTATATACCTGATATACCTGCTCCACATGAAGCCAACGATCGATATAATTGAGCGAGATGCGCTATGTAGATCAAATTGTTGATCTCTTTTCTCACTTTTTGATTGACTGACATGTGGATGCATTTATTCTGCACTCGATTTGTCAGGCGCCAAAAGATTGGGCATATAAAAGTTTGTTGTGTTCATAAAATGCATTCGCTAAGTGGGTCACTTGCGATCGATAGACAATGAGAAAGAAAGAGAGCATGAGAATCTCAAATATTTCACTTGGAGACTACAATATAAAGTATATATTATTCTGAACTAAAACTTTAGTTGATAAGAGTTCTAATAAGTCTTTTTCATGACACAGGCTATCTTATCTGAAGCGATTGCGAGTTCTTGAAACTATCGACTGGATTATTATAAGTTGGAAACTAAGATTTTGCAAAAGATACTTTTTTATTGAACTTTGCCTCTTCACAAGTATTTAGATTAAAAGTGAGTTGAATTTACACATGATAGAGTGATTTTCTAGTTTGCCTTAAGAACAGTTTAGATTTCACTGCATGTGAGTGGAATTGAAAAACAATAAAGCAACTTAAATGCAAATTACGCATTACGTGTTCCCAGAAAATTACCCAGAGTGTTCCGAAAGT AACCCAGATGAGCTACATAACCAAAAGTCCAGCCCCAAAAAGAAAAAAAAAACAAAACTGTGCAACAAGTGGCTATTACAAAAGGCTGGACAGAGTGTTATTTTCCCTCCCAAAACCAGAAAAACAAAAAGCCAAAAATGTAAATGAAAATGAAGCCAACAAATTAGCAGACAAAGAAACGGTAAAATTGTTTAAAGCCAGAGACCAATTCGAAAACTAAGCCCAAACCAAACAGCCAAAAGTTTTCGTTAGCCCGAATTGAGGGAATGAAAACGATTAAAACAATACACTTAATACGCGCATTTGTTTGTATTTATGTTTTGGCTGCTGGTTTGGTGCCCATTTTTTCTTCGAACACATGTTTTTACTTAACTTTTCAGTGCTTATTTATAGAGACGACTGCGCTGGCTTTTCAGCTTTCTGTTTCGTTTTGTATGCCTTTGGCTGCCCCGGCACTTCTATTTGCCTTCTGTTGCCCTTTTCAACACATTAAGAATTTTGCGTCAAAACGAGTGCGACGAGTGACAAATGGCCACCTGAACTCCGGCTCCAGAATGGCCAAGAAAGTCCAAGACGCCGTTTAGCGCGCTTTTCAACTTCTTTTTTGGGGCCATCACATTATGGTAAGTACATACATATAAGTTGAACTCAGTGGCAAAAGGGCAGGCAGCAAATGACAAGCCTCTTAGAGGCAAAGGAAACCCAATACGCCACAGATTTGTATGATTTCTACCCCATCCTCCCTTTTACAACAACCATATTGACACCATGTGCGCGCCACTGCGAAGGGTCTGTCCTTGTGTGTGTGTGTGTGTGTGGGTGGGTGGTGATCGCCCACGGGGCGTATGATTGACATCATGTCAAAATTTGTTTTTGGCCCGCATGACGAACAGCACACTCG
 

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