CR01346

CRIMIC CR01346

Gene CG5080

CG5080

Information

Donor Location Cytology FBti Stock number
pM37-p22L:1162593..116261021F1FBti020690383275

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK no band

Images

Construct Design Sequences

sgRNA sequence AGTGCATTTGTCGGCGCCTGGGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
CTGGCCGACAACTTCTGGAT TGTGCATCATGGTTGTGGG GCGCCGACAAATGCACTTAG ATCGCCGCGAATATGTCCAA
 
Amplicon 5' Amplicon 3'
CTGGCCGACAACTTCTGGATCTCCTCGAGCAGCTTGGCGTTGAGGCGCTTCTGATCCTCCAGGAACTCCTTGGGAATGGCGGGTGGGGCGGCCGCGGCTGGGGGAGCGATCATGTTGGCGCTGACCGGCGGCCTCTTGAAGCACTCGTCGTTCTCCCGCATCCACTTGAACATGTGCTCCGTTGCCTGGTTGAAGCGGTCCGACTGCGAGTTGTACTTCTCCTCTTGCTGAGGATCGGATAAGTTTGGATGATAACATCTACTACCCCTTTATGGCTGACTTACCGTGATCAGCATGGTCTCTATCTGGCTGACCCGCTCGTCCAGGCGCCCGAAGATCCCATTGATGGGCTCCAGGCTCTTCTGGCCCTTCTGGAGCGACTGCAGCGCCTTCTTGAGCAGCTCGCCCAACGCACGCTCGCGATGCTCGTGCCGCTCCAGCTTATTGTCCAGCTGATTGTAGGAGTAGATCAGCGACTGGATAGTTCCGCGCAGCTCCTCGTTGCTGGAAAGGGTAGGGGGTTAGGGTTAGTGGCTTAAAATTAGCCTCGCTGCGGACCATTAATCATCGGAGTGGGGGAGGGCAGATGGCAGCGGGACCACCACCATGATGGCGCGTCTATAAATATTAAGGGCTGGGATCTCAGGGTCAGGAGCCCTCCACTCCGCACGCTGTTTGTGTGAACTGCATTGATGGCGATGGATGTGGCATGGCTATGTGTGGCTGTGGAGGGGAAACACGGAACACATGCCCATTTGCATGGGCGAGCAGTGGGAGCTCTGGGAGCACTCCCCCATTCCCAGCCAGAGTCCCAAAATAAACAAACAAATAGGCGCAGCGCCAACATCTTGGCTCCCTTTGAAGGCACCACTCACTACTGGGGAGTGGCTGTGCATGGGCTGCATCCATACTCCATACTCCATACTGCACGTCCCACAACCATGATGCACA GCGCCGACAAATGCACTTAGTTTGAAGTCTGGGCACAGTGGAGGGCATTATGAGCGGGAACCAGAACCACTCCGCGATGTGTGACATGTGTCAAGACCATAAAACCGGAGGGCTGCAGTTGTGGTTCCTCGAATCCACATCGGCCCCTCCAAGAGATTCAAACGAAGAACTTGAAGTGGCAGCGGATCAATTGGGTCTAGAAACTGAAGATCTGAGTGATTTTACGACGCTAATGGAAGTTTCCGATGGAGTTGGGCAATCAATAATGTGTATGGAGAACCAGGAGTGTTGGTATTTAGTGGTTTAAGCGAAGGGTTCCACAATGTCATTGGACCAAAGATCTATGTACTTATTCAAGCTTTTCCCCGAAAATGAATGAAATCTACTCACGAGACCAATCCAGCGGCGATGGGTTGCCATTGGGCGAGCGCAACTAGGACCAGGAGAACTAATTGGAGAGGGCGACTTCCTCCGGCCATCGTGGGTTTGGTGCGTCGATGCGATCTTAAATTGGTAAGCGAGTCCACTGCGTGGGGTGACCTGTGGAAAAAGAAATCCAAATCGAAATCGAACGGAAACGGGGAATTGAGGATGGGTAGCGATGGCGCCTCCGTTCGCCGCCAGTGGATGCGGATACTTCCTCTGGAGCTGCGTCGGCGAATGTTTATCGCTAGCGACAATGGGGTAAACAAATGTAGGCTTATTTATGTGCAATATTTGCGGCGGTGCAGGCGAGCAATAAAGAAATGTCAGTGGAACAAAGCGGATTGGTTCCTTGTCCGGATGGACCTCGCTAAAAATACATACATATGTGGATGCGATATTGTTCTTGTGGCGATAAACAAATGACGCGAATTATCCGCTGGACACTGCATGTGCCAGATTCATTCTCCTCCGCGGGAAGATATGGAGGAAGTGATGCATTCGGGAAATTGGGTCAGAAATAAGCGAGTGAAATTCGCGAGGGCGAATCGAAATTACAATAAGACCAGTTGCCATCGCAATTTCCCCGGAGATTTCTGCTGTAGGTTGGACATATTCGCGGCGAT
 

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