CR01324

CRIMIC CR01324

Gene GluRIIC

CG4226

Information

Donor Location Cytology FBti Stock number
pM37-p12L:914725..91476021E2FBti020689683268

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence CCAACAGAGCGTATTCGTCATGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
AAAGGAGATGGCTGGAAGGC ACAAATCTGGGAGATGCCGT TCATCTGTTTAATGAGCAGACCT ACGTTATCCGCTGTCCATCC
 
Amplicon 5' Amplicon 3'
AAAGGAGATGGCTGGAAGGCCTTCGCTGTCGTCTGGTTGCCATGAAAAATTGACACCTCATCGCCTTTCAATGGAGCACCTGGAACCGGGAGCTCCGCTCGTGGGTGAAAGACTGCGGCTGATTGAACCGTGTCCGGCTTTAATGGTCTCTGCTCCACGGGGAGCCAATCGCTGATCCTTCGTCAGCTAAACAAAACTAAATTAAATATTGGAAATATTTGCTTAGAATCTTATATAGTACAGCATGTCAGCTGACAGAGCTTTCCCAGTCAGCTGATCTCCGATCTCCACTTGTCGCATTCGATCTCCAATGGCCCGAAGAGCTTTCCCAGTTGCCATGGAATCGCCAGCCCGTGTGCTCGCATGAAAAAAGAACTGTCCGGAAATAGCTCAGCCCGCTGGCCACAGATGTTCGGTGGGAATTCCAGAGAAGCAGGCATGTGGCAGCGCATACTTTTGCTCGGCTGCATGTGGAGTGCATTTTTCATGTGCCGCAGCCGGGGGCAACAAATCAACATTGGTGAGTTGTGGTTAGGAGTGGGAAAAAGCAGCTACCTACTTTTTTGTGCAACCCGCAGGCGCCTTCTTCTACGACGATGAGCTGGAGCTGGAGAAAGAGTTCATGACGGTGGTCAATGCCATCAATGGACCTGAATCGGAACAGACGATGCGTTTCTATCCGCTCATAAAGCGCCTGAAGCCCGAGGATGGGAGTGTCACAATGCAGGAACATGGTGAGTCCACCCATCCACGCACTCTATTCCAAATAACTCGAACATATTTTCGCTTTCAGCCTGCGATCTCATAGATAATGGTGTCGCGGCTATTTTCGGACCAAGTTCAAAGGCGGCGAGCGGTAAGAGAGTGCGGGAAAATCTTGTGTAGAACGCGAAAGCAAAATAGAACGCGAGGAAAATTGGGCACAAGAAAATCGACGAAAACTTTGACGGCATCTCCCAGATTTGT TCATCTGTTTAATGAGCAGACCTCTACTCTCTTTTTGCAGACATTGTGGCCTTGGTGTGCAATAGTACCGGCATTCCGCACATAGAGTTCGACATATCGGACGAGGGCATTCAGGCCGAGAAGCCCAACCACCAAATGACCCTTAACCTCTACCCGGCCCAGGCCATATTGTCCAAGGCCTACGCGGATATTGTACAAAACTTTGGGTGGCGCAAGTTCACCATTGTCTACGATGCGGACGATGGTGAGTAGGAGCATTCAAGAGAATTTGTTTATATCCAGGACGAACTTCCCACAGCCAGGGCAGCAGCTCGACTGCAGGATCTTCTGCAGCTGCGAGAGGTTCACAATGATGTGGTGCGGGTGCGAAAGTTCCACAAGGATGACGACTTCCGGGTCATGTGGAAGAGCATACGGGGCGAGCGGCGAGTGGTTTTGGACTGTGAGCCCAACATGCTGGTGGAGCTTCTGAATTCCTCCACTGAGTTCGGTCTGACTGGACAGTACAATGTGAGTAATCCATGGGATCCACTAAGAGAAACAGCTTTTATAAACTTTAAAACAGCATATATTCCTGACGAACCTGGAAACCTACACCGATCATCTGGAGGAACTAGCGGCGGATAACGAAACATTTGCCGTGAACATAACTGCTGCTCGTCTTTTGGTCAATCCCGATCCTCCGCCGGTAAATGAAGCAGACAAAGCCCTATTCCCTTGGGCACACTAACTCATTTTGGTTTCGCAGTACTCCCTACCTTACGGATATGTCACCCAAAGGGACAACATCGTTTACGAAAGCAGCGATCCGCCTCGCACCCTCATCCACGACTTGATACATGATGCACTGCAGCTTTTTGCGCAGTCCTGGCGCAATGCCAGCTTCTTCTACCCGGACAGGATGGTGGTGCCGCGAATAACCTGCGACTTTGCCGCATCCGGAGGTCGCACCTGGGCCATGGGGCGTTATCTGGCACGCCTCATGAAAGGGGTAAGAACTACTTTCCGCATAAACCGAGGAATCGAAACGTTAATCTGCGAGGTGTGTGTTGCAGACTTCCGGCGTGAACAACACGAACTTTCGGACAAGTATCCTGCAGTTCGACGAGGATGGACAGCGGATAACGT
 

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