CR01320

CRIMIC CR01320

Gene S

CG4385

Information

Donor Location Cytology FBti Stock number
pM37-p12L:1056584..105659721E4FBti020689383265

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
no band OK

Images

Construct Design Sequences

sgRNA sequence AGTAAGTACCTCATAAGAAGTGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
AATCCGGGATCTGGAATGGC TGAGGTACTTACTCCGCCCG CCTCTAGTCATCGTGAGTCGC TTAGCCCATTTGTGCGCAAC
 
Amplicon 5' Amplicon 3'
AATCCGGGATCTGGAATGGCGTGCTCGCCCTGGATCCGCATGTCCTATTGCCATTGCCTAGTAGTTTGGCTCGGTTGGTCTATTGATTTGTGCTTCTTGTCTGGCTTGTAATTACAAGCTCGTACGCCGCCATTCAAAATGGCCTGTTTCCATGTTGATAACGTGAAATGTTCCCATCCATGAACACGTCTTGCATGTGGTAACAGAACAGGCAACGCAAGCGCTGATTTATTTAGCAAATAGGTCCAAGTGGGCCTAATCTATGTGGGTCGGATCTTAATCACCGCCTGCAAAGCGCATTTAGTTGTGGCGAAATGGAAAAGGCCAATGCAGGGCAAACATGTACGTCACGTGATCAGGTGTGAAATGCATTAAGTGGGTGGAAAAACAAGGGAAAAGTGCGATTTAAAAGGTTGAATCGAAAAGAGCACCAACTACTAGCGAATGAAATGAAATATTTTATTAAACATTACCAATTCAGACTGATATATGACAGCGTGGCACTCTGTATGATGATATATGACCCATTGAGACGTGTCAAGAAACCTTTGGCATGGTAAATGACCCCGTTTTAATGCGTTAAACGTTCCCTTTAGCCATTAATCAACCCCGATTGAAATATCTTCCAGCGAAATCCCGATAAAGCAGACACGAGACCGAGACCAGTTGTTCAGCCCCCGCTGTTGTTGTTTTCCTTATTAACCTTATCGCAGTGGGCACTTTTCCAAGGAGAACCCACACGACGCCTAAGAAACGGCGCAATTGCAATGCCACAAGGAGGGTTGCAAAACCCGAGACCCACATGCGGGTGGTGGCTCTGCGGAAAGAAGGCAGGCGGGCGGAGTAAGTACCTCA CCTCTAGTCATCGTGAGTCGCCGTGGCGCATGCTGTGATAATGCCGTTTGAGCTTTGCTTTTCAAATTGAGCACTCGCTGCGCTTTTCACGGAGCTGATCATCTCCGCCGGAGGCTCGAACGAACCGGGCGAACCACTTGCACCACCGCGACGAAGCGCAGACCATGCCACGCAGTCCAGACGACGACAGCGACGCCTCTGCGGGGGTGGCTTTTTCCCCCCCGGGCTGGGGGCCATGCAACAATGTTGGCAACTGCACACGAAAATTATGAATCACGAGGCAAACTAAGTCGGTGGGAGGAGGGCCCACAATGACATCCGGTGGCTAGCAGGGGAGGTTAATGCTACACACGAAGAAACAAGAGGACAAATTTAGTTAATAGTCGAAGGTTATCAGTCGATAATGTGAGACTTTGTTATAAAAGGGTCGCTGAATAAGGCATATCCAATGTAGCAAAGAAATCTTTTAAAGTTCCAAGATTTTAATTTAGATGTAGGATCAAAGTCTTAATATTCTGCAAAGTACTTAAGACTGGTTAGAAACTGCCAGACTAATAGGGGATTTGTCGTGACCCCTCCCCTTCCACTTTGAATTCCCTACGAACCCGCTGCGTGCCTAACGGGAATTAAGTCATTAGTGGTTGGTGTGTGTGTGCTGTGTGTGTGGTGTGTGTGCAATGGAGTTCAAGTTGTAAATTTCAATTAAAGTTGCAATTTGCAAATTGTCACCAGTGAAGGCCAGACGACAGTGACGACGACATCGTGTAATCACCGCAAGTGTGAAGATAATGCCGTCCTAACATGATTAATGCCCTGACCTTTGGGGGCGTGTTCGCTAGCGTTCGAAGTGCACGGCAAAAAAAACTAGTGTGGGTAGTTAATTATATTGTAATGTGTTGTTACCCAATTCACAATTTTACTTCCTTGTTATCGAACCATAAACATAGTTGCGCACAAATGGGCTAA
 

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