CR01302

CRIMIC CR01302

Gene vkg

CG16858

Information

Donor Location Cytology FBti Stock number
pM37-p02L:5025521..502554625C1FBti020688683258

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK no band

Images

Construct Design Sequences

sgRNA sequence GATGGCAAACACGTGAGCGATGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
CCCCTCACCGATCACTTGAG AAGTGTGCAGCTCGTCTGAC GGGCCAGATTTCCAGATCCC CGGGGGTTCAATGAAGTCCA
 
Amplicon 5' Amplicon 3'
CCCCTCACCGATCACTTGAGCTGCTGCTAATTGTCGGAATCGAACTGCGCACACACACACAAGCGCACTGGCGAAAGAGCACACTTATTCACAGGCAATAACGGCATTTCCATATATGTATTTATAGCTGGGCAAGTGCAGGAGAAAAGAGAAAGGGTGCATGGGGTAGTGGTGCAGTGGTAAAGTGGGGAGAGCGACTGATAAAGACCATTTATCAAAATTCATTCATTGAAAGCCTCAGCTGCATCTGCAATCGATGAGTTCATCTCTCTCTGTCTCTTCTGCTCTTCGATTGAAGCGGCTCTTAAGCGATCGGCTCTCTTTTCGCGGAACGGAAAAGGAAATCATTTGTTCGGCTAATGAAGCGCCAAACTGGTTGCAAAACCCATTGCGAGAACAGTTTTAGAGCCATTGCCTGAGAGCTGTTAACTGTTTAATAAGTATATTCAAATGATGCTGGAATAAATACCCTTAAAAACATTAAGTATGAATATATCTTACTCATAGCAGATAATTCCCTTTATAAATTTCTTTATAGATTGTAAATAATGGCATACATACAATGTGTGTTTCTAATCAAAACTAATTCCAGCGAAAAATGTGAATCCCCTTCTATTCAGATTAGCTTAATGCACCCAGGATATAATTAATTATATCGTTTTCAAATACTGTTGCAAGTGAAACAACTTTTTTTGTAAGCCTTCGCTTATCGTGAGGGTAACACGAGCAACGGTAGCTAAGCGAAGTCAAGTTTTTCATCGTTTTGCGGTTAGTCCTCCATTGGCAACCCACAAGACCTGGAGACCTGGCAGCTCTCACTCGGAATCCGGGAGTTAGGGTCCCAAGCCCATGGCATTGACCACGTAACCGAGTGCGAAAAGAGCTGTGGATCGGATTACCAAAGTCGTCAGACGAGCTGCACACTT GGGCCAGATTTCCAGATCCCAACGAATTGAGTGCACGAATTGTGCATTGAGTTAACTGCTCAACTGGTATGAAAACTAAACTCTGATCTCTGGTAAAGGCAATAAAGTCAAGCCAAGCCGATAGCCTCTGCGACTGCTGGCGACACTTGTTTCAATTCTAAAGTGTATTTATTCTAAATTCGAGTCTAGAAAGCCATGTCGCAATCAGCGGAACATGCTATCAATGGCGTCAATGCCGCCAACATAGCAATAGGTCTAATTAAATGTGTGTAGATCAGTCGCAGCCTGATAAGACCAGCCGAATGCCATCAAAAAGTAGGTGCCGCCGGCATGTGCTCCATTTTTCATCCAGCATTACGAAATCTCTGTGATCCCTAGCCATTTAAATATTAATACTCAATTTAATTTGGTACATACACAAGGTACACTGAATTGGGCAACTCGGTGTTAAAAAGTAGTGCATAGTTTTTGGGGCAACATTGAGTATTGCGTTTGCCTCGCACTTTTGTGTCAAATCATTTTTGTGTTTGCTTAAAAAATCATTCATAAATAAATGAAATATTCGCAACCTCAATGGTTAATGTGTCTTATTTTGAATAATAATCTATTCAATTGGGGTAATAACTAAGAACCTGCGCATTCTTTGTTTTTCATACTCATTCCCGTTTCATTTTACAAATGATTATATGCCAATTAAGCGACTAATCTGGCGGTTATTAAATATAATTCTAATTGCCAGCCGGAAACGTTCAATGGCGAACCACAATTGGTATGCCAAAAAAGCAATGTCCTCCAAATTGAAAGATTAGGGGGGTTATCAATGGGCGTTATCGCCAACCTCTTCACCTACAAAATTATGTTGCCAAAATTATTTAATGACATAATCGATCCCAAGCTGGACTTGGACTTCATTGAACCCCCG
 

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