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CRIMIC CR01151

Gene CG10936

CG10936

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:17629396..17629396	54D2		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GATCGACTCTATCTTTGCCGAAGAGG	CAATGGAGTCTCTCCTTCGCTCAGAA

Images

Construct Design Sequences

sgRNA sequence	GTAGTTAAGGTGGCCCATTGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTCAGTTTTGGGGCCACACT	TTGCGGCTGCCATCGTTG	ATGGGCCACCTTAACTACGT	TCGTGCAGAAACTCTGGCAT

 

Amplicon 5'

Amplicon 3'

TTCAGTTTTGGGGCCACACTGAGCCCGGTTGACTAAAGTTCGATTCAATGACATTTAAACATTTAAGCCGGGTGTCTTAGCCAATGTAGTGGCAGCTCACTTTTGTGTGGTTAGAACAAGATATTGCTTATAATATTGATTGAAATAACTTATGTGGATCGTTAGGAAAAAGTGGTTTGACCTTCGAATTTAAATTCTCTAATCGCTGGCAAAGACATCACAAAGTATCAGTTGTCAGGCCAAAAACAAAATTTATTGGGGAAAAAGGTTAGTATATCAGGTTACTTGAAACCATTGATATGTACATTTTTCAACATGTACGGAGAAAACATAAAACATAGTATAAACACAACTCGGTGTTTTTCCTTTGAATAGATCAATTTGATGGGTCAGCTGGAGTTTACCCAGCTTAGATGCGATGACCTTGAACTTGGAGTCGATTATGTTGTGTGATGTGAATTTTTCTTTTTGGCCAAAACGTTGTACAAACCTAAAAACAAAAACAAAAGAAAAACGACATGAAAAACAAATTTTTTTTATTGCTACAAAAACTTATTTTAATGTTGCACTACAAACAAAAATAAAAGCACTCATAAAAACAAACAAAGGCAATTTAATTATCTTAACGGGCGTGAAATTAACATTAAGAGGAGCTGACCCGCTGTTTCAGGTTCTTGGGGCGTGGTCAACAAAAAAAGCGAAAGAGATATAAGCTCGTAAGAAACAAAGCAAGGTCGCCGAAGACGGGGATGCACTTGCGTTTAAAGAAAAGGGAAAACTGAACTAGTTTTCCGAGGTCAAGCGATTAAAGATATAACAGTGAAAAATAATTGAATTGAGCTTATAAAAGTGAAATATACACTTGATCAATGCAACACTTGGATCTACATATATAAATAGCTTAAGTAATTGTTAGCTTGTCAAGAGTAATTTGAAGCTCAGAATTACCAAGACCAACCTTTGAAATCACTCCATCGGGTATTATCCGATGATAAATATCCCATCAACAATCAATCAGCTCTTGTAATGGAGCCAACCAAATTTAGCGCAGCATCGGTTTAACCAACATTGGGAAATCTAACCACATCTCCCGCAGCTGTTGTTGCTGCAACAAATATGCACAACGATGGCAGCCGCAA

ATGGGCCACCTTAACTACGTATAATTGCTGTTCCAATAACCCCTAACCCGGCGGCACAAAGCACATACACAAGAGATGCCAAACAGCGGCAGCAAATTGTGACTCGTGCCAACAAACAACAAGCAGATATATATTAAACTGTCAAACAAAATCATAAATATGGAAAACACACCCACTCTCTTGCCAATCGAGTGAAAAAAGCTAGTTCACTTGGACAGCAGATGGGGCAGGATTAAATTGCAGTGTGAAATGCAGTAGGAAAGATTAGGGGAAGGGAGACGCTCCTCCTCTGCATATGACTGCATATTATATTATTGTATCTGTTTGCTGCCCCTCAACCTAAGCGACCCAAAATGTTGCTGCTGGCCGCAGTCGACCACAAAACAACATCAACATCCAGCCAAGAAATGCGTTCGTTGCTGTTTTTGGTAATAAACGAAGCGTTAAACAAAATGCACTACAACAGCAGCAACAACCAACCAACCACCACCAACAACAACAACATAGAAAACAAACATTTTGTGTCTTTCTGATTTGTTGTTTACCATATGCAAAGCGTCATATATGAGGTTGCCTACAGTTGCAGTTGTTACCGGTTACTTGTTGCTGCCTTGGGCGTAATTACATCTCCAAACCAAAAATGGTCAAACGAGCGAGAAAATTATCACATACGAGGTCCTCATTGTTGTTGCTGCTCTTGTGGCGGCAGAAATTAAATGCAAATTTTGCCTGATAAAGAAATATGGGGTTACAGACGAAATTTCACAATCAGGAAATTAAGCATTTTTCTTTTTCGGCTGTCTCGAGCGGTGGAAGCCAAAATAGCCTTCGATGATTTGACATCTGATAGGTAATTTGAATATTTTTGGTAGGCCAATTAATGAGCGGAAACTTTTACAAGCATTTTGAAAGCATATGAGGGATACGTTTCCCTTTTTCTATTTCGCCTTCCATCAAACACTCCTCGGAAATGCCAGAGTTTCTGCACGA

 

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