CR01129

CRIMIC CR01129

Gene Sb

CG4316

Information

Donor Location Cytology FBti Stock number
pM37-p23R:16139950..1613996289B5Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
no band OK TTTCTGGCTCAAAGGAGTCTGCAGAC TCTTACCTGATCATCGGCTTGTAGGG

Images

Construct Design Sequences

sgRNA sequence TATAAGGGGGCACGTGCAACTGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GGCGATAAACGGGATTTGGC TGCACGTGCCCCCTTATATAC AGCGATGTGTGCTACCAGTT AGCCGCTACTCATGTCATCG
 
Amplicon 5' Amplicon 3'
GGCGATAAACGGGATTTGGCTGGAGCCGAGCTTTGAGTATCTCTTGTATGAGTGTGTCGAATGAATGATGTTTGGATATCTACGTTTTCCAATGAGTGTTCGATAAATATGAATTAATCCATGAGAGAGGTTTGCTCCGTACAAATTCGTATAAATATAAATTCATTTACATATGTATTATTTGGTTTTTAATAGTTTTTCTTAACCTGTATTTACTTACCTTTGTCATCGGAACATTTTAATCATAATTGATTAATTTATTTAGCCCATTTAAATAAAGTTCTAGCAATTACTGACTAATTACTCACTCCAACATTCATTCGCAAGTTCCTTCACCTCAGCCATCCATGAGTTGCATAATATTCCCAAGTGTCACATAATTGCCACAATCCCGCCCCCAAAGTGTTGTCAGCACATCCATTCACATGGCTCCTTTCCGCACTCCTCCAGTTATCATCGTTGCATATGACCATCATCATGATGATGGCGATCTTGTGATTACGATCTGTGGCCGGCTAATGCCGCAATCTCGTGTGTGTGGGTTGTCTGGTGGGTGGGCCGTTTGAAGCACCACCAGATCGACCGGCACCACCACATGCATGCCAAACTAAAGTTAGACATCCCGCAGTTAGCCTACTAATTACAGCCGGAGCTCTTTGCGCCCGGCATTCTCGATGCCATTACAAACATAAACCATAGCTTGGCGCAATAAAAAAAGAGCATACGGTTTTCTAACAACAAACTTTGGCTAACCATACTGATTATTACCATTCTCCGTGCAATCCATTGCAGACATGCGCCCGGCACGTTACCGATGAAGCAGCCAACTTTAATCAGACCCAGACTCAGACACAGGTGAGTTGACAACTCGAGGGGTGGTGGGGATGGTTGGATGGGGTATATAAGGGGGCACGTGCA AGCGATGTGTGCTACCAGTTTAGGTTACAAACCATAAAGGCAAACATCTAATTTGGCCAGCAATGGATTGGCTAAACTTTAATTAAATTTGTTGAAAAGAAAAAAGAGCTATTCCGCTTTGATGAGCTGCAGCGAAGAGGAGTACGAAAGGGGTGGGGCAGCACTGGGGTGCACTTGGTTTTGGGACAAGAAATTATTAATTGTAGCATGTGTCGTTTGACGTCGCCGTCGTGCGATCGCAATAAATTTTTTCCAGTCTTGTTCAACAATAAAACAAGACAAGAGCCACGAAAAAAGCTGATTATGAAGCAAAAGTGAAAAGTTGTAATTAAACCAGTCACTGACTGCACGGCGAGCAGCACAAAAACAAACTGCAGCAACATATGCAGCAGTAGCAGCAGCAACTGCAGCAGCAGCAGCAACAATCATAAAGAGTAGAAATCGTTGCCTGACTTTGGTTTTTTTTTTTGTGCTGCCACTTTTTGTGGCGACAAGTGCGCTTAATTAGTGGTGGACTCAGCGGCGGGGGGTGGTCCCAAACCCAAACAGGGTTCTGGTGGATGGGTTCCGGCAGGCGAAACAAGACTAATGTCCGAAAACTGTCATGCCCGTTCCTTGCAGAAGAAGCACACCAGCGGCAGCCACCAAGATGTGTCCCAAAAGGCATTGGCTAGTCAACAACAGAGCAGCGGGATCGCGAGGATCAGGAGGAGCTGCGGCGCGGAGCAGGCGCAGTCTAGACCAAATAGTAGAAGTATTAGTAGCCTTAATCGTAGTCAATTGCCTGGCCACAGCAGCAGCGGCGCTGATCACACCGCCCGACAGCCTCGAGTCACTGGGCTCCCTGGGAATCCCGTCATCTTCCGCCTCCTCCTCCGAGGACGACGATGACATGAGTAGCGGCT
 

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