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CRIMIC CR01040

Gene CG10211

CG10211

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:18521013..18521030	36F6	FBti0206880	83250

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTGCCAGATGCTGACATTGTCTAGT	AGTTTGTTGGCCAACTGCCACCCAAT

Images

Construct Design Sequences

sgRNA sequence	AGAGTTATACGTGCTCTCTCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCTTCAACGGTAGCAATGCC	CAGGGTCAGCAACTAAAGCG	TCTGTCGTTGGCCAATTCCA	ACAACGCACCCTGGTGTTTA

 

Amplicon 5'

Amplicon 3'

GCTTCAACGGTAGCAATGCCAAGGGGGTGTGGGTTGAAATTGTGAGATGACATGATTTTACACGTGCCTAAGAAATGAGAAAATGTGGAAGGGCCAAGAAGCTATGAAATTGAGCAAAAGATTTATTTCCCATACCATGATAATTATTATGTTCATACCATTATCATTTAACAAATCCCAAAAAATATTTCAAAAACATAGATAATGGCATTAAAATTGCTGGGTTCTAACTCTAATCTTTAATCAGATTTTAAGATATTTATCTCTCTAAAACCAAACATGATAGTTTAAAATTGTTCAAAATATTAATGATCTAATTTATTTTGGATCCAACAATTCCGCATCTTCACATAGTTCTTCAATCCCACTTTAAGTGTTGTCACTGTCATTAATCTATTTCACAGAAATCCATTAGCACCTTTCCAAAAGGGAAACTTAAATTTAAATTAACTTTAGCGCCGCCTCTTCGTTTAACACATTTTTGTGAAGAAAATTAGTCAAGACATTTTTTTTGCTGGCTAGAACTGTTTTAAAGTCAATTAGCCAACGTGTATATGATAAATAAGTAATTAAGAGAGAAAAAACAGAACGGCGAGTTCAAGGCTGAAACGATATCTGAAACCGAAACCGAATTCTCTTAGTCGAGAATTCGTTATTTTATGTGTTTTTGTAATTTAATTTGCAAGTTTTAGCCGTCTTTCGATGGGCTGTCATTTATCATATTTATTTCATCCGTTGTTTGCTGTGTCTAAGACACGTTTTTTTATTTATGTATGTCCATGTCTAATTTATTGACTAATTTATCAACATTTGTCTGGGCTGGGGATCCAACACCGAAAGTTGATCCAGTGAAAAGTTGAATAAACCTTTTTGACATGTGACTGTCGCTTGATGGCCGAGAATTCCTTTTCTTTCTCGGCCTTCCTGGTGAAAGGAGATCAAAACTAGAGATCGCTTTAGTTGCTGACCCTG

TCTGTCGTTGGCCAATTCCAAGTGATTCGATAAATCAAGATCCGTTGCTCAACTTGTTTTCTGCTCCGCCAAGAATAATTCAATGCGCACAAATATTTCTGCGAGTGTCCAACTCTGGCAGCGCCTGTCTGACCACTTCGCTTATTTCTACATACATGACTCCAAAAAACGGGTTACCCCAAATAAAATAAAAGTATAACGACCAATATGCATAGCAGCTGGATCAAGAAAAAAATCTCAAAGGAACGGGTGCCAATTGTCTTTTCAATACATGTAGCTGGTGGTCTCTCCCTTCAATACACAAAAATGTGCTACATTCAACCGCAAACAATATACAAAAATTACATCCATGCATACATCTTGTTAGCGACTGGTTTATTTGGGTTAAAAAGAAATTTTTTGGATGCGTTCGAGCTCCCCTCTTTCAAAAAGATAACGGAACAAGACATCTGTGAAAAAAATCAAAAACCCATAAACAAAATCTGAAGCAAAATGCCAAACACAAGAAAGTGAAACTTTTGTTGCTGCCATTGTACGACGTAATCAACTTCAACAGGATCAAGTGGCCAACAGGCTTGACTGGGCCTCATTTTTTCGGGTTTTCAGTAATTTCGCTCTCCAGCAACGGAACTAAATATTTGTTAATGTGTCTAGCTCTTCAAATGAAGTTTAAAGAGCCTTTTGAGGTTTTTGCTTTTGGCACTTGCCTGCCTGATAGCAACTGGCATCTTCCGTCATTTCAAGGCGCTACAACTGGTTGTCATCTTACATAAGTCGAATGAGGATGGGTGAAATATGATGATTATGATCAGCGACTAAATATATGAATTTTTTAATTTAAAGCACGGAACTTATGGAACAAGGGGCTGGGGTCTTGTTTTGTTTTGTGCCTTATTATACATACTCAATACAATTTAGATAAATTTCTGTCCCATTTTCTGTCTGTAGATTTTCATCTTGGTTTCCATAAACAAGCCTTCATTTAACTTTCTCACATTTCACCTTAAATTGCCCGAAAAGATTTCACAGCATTTGGCTGCCGGGGGGAACACAAAGCAATTTTTTACCATAAACACCAGGGTGCGTTGT

 

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