CR01025

CRIMIC CR01025

Gene Sema2a

CG4700

Information

Donor Location Cytology FBti Stock number
pM37-p12R:16522143..1652212153C6Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK AATTAGCACAGGCAACGGGCCAAATG TCTTTTGCGATTTTCACTGGGCGGAG

Images

Construct Design Sequences

sgRNA sequence CTGAAATGCCCCCGGGGCATCGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
CCTTCCCTTTTGTCTGGCCA ATTCCACATTGCAGCCATGA AGACGGCTTACAGTGCTGAC CACGTGGCAAAATGGAGCAA
 
Amplicon 5' Amplicon 3'
CCTTCCCTTTTGTCTGGCCAAAATATGTTGCATATGGCAAGGAACTTGCGGCTTTGTGCGCACTTAAGTGGCAACAAGACGTGCAGCTGGACTCTGTGAATTCATGGAAAATTAAGCGAGCCGCAGAATGTTTTAGTTGCTGGGCAATTTTCCCATAAAGGTGGCCAAAACTAGCAAAAAAAAATAAAAATAAATGAATACCATCTGGGTAAAAAGGGCTGAGGGTAATAGAGCTGTAAATACTCATACGTAATTGTACACTTAAATAATACAGGATATATAATCACATCATTCAAATTTCTTTTATGAAATTCAAACTTTTTTATATTTGGTTAAAAAATTGAAATGGCTGATATTTTCAATAAATACGGCTTGGTGTTTGATTACGATAATTTTTTCAACTATCCACTCTAATATTCATTTGTTTTCTTTTACCTGCCCACATTTTTTCGCTCATTAAACTTCAGTTGTATATGTATATATCCTGTACAAGGGTATAAAAAGTGTGGGTTTTAAAACTATTTGAAGCGTTTATGTGGCGCACTCACACATGAGCCATCAAATGAAGTTGCACTGTGGAAATACCTACCTCTGCTACCACCTCGTTTTTAACCAAAAATGGAAACAGTTACCGCATCCGCATTTTGTTGCGTTTTTGTTGGCAGCTTTTGGGCGATGTCCAGGCCATATAACTAGGATACTCGGATGCTGGGATGCTGGGATACTGGGATGTCTAGTGCTGTTTTGACTGCTCTTTTGATGCTTTTTTATGAGTGCGCTCTTCGTTTTTTTTTTTTTTTTTTGCATTTCGCTATTTTGCAACAAAATGAACTTGCGCAGCGCATTGCTGCTGCCATAAAATGAACTAAATTGAGTTGAAAATGTTTTGCACAACTCTTCACCCCCCCAAAAAACTTCCTCTACAGTCTAAAGCATCCCTGCATTTCATTCATGGCTGCAATGTGGAAT AGACGGCTTACAGTGCTGACAATAAGTGTATTTACCATGCATATGCGTTTTAATTATACACACAGACAATCAAAAGGAGACAGCCACCTCTGGGTTTGGGGCCATTGTGTGTTGTCTTGTGCAAATGAAATGGTAACCGCTTTGGCTAAGCATTTTGGCTTACTCCCCACACTGCCAATCCCCCTCTATTCTTCTACTTTGGCTTTCTCAGCCACACCCACTACCGCCCGCCCGCCCTTTTCACATTCAGTTTGCAGCACAGGTGCAGACGTCTAGACGGCTTTCGAGTGTTTAGAGCCGACGTGAATTTCTCATAATTAGCAGAGCACTGAAATAACTGGGAATTTTCGCCAGGGAAATTCTGGGCGGGCAATGCAAAGCAGGGAAATAAACAAAGTGATTGCTTTTATTTCATTCAGTAGATAAAAAATATATATTTATTTTTTAAGTTTCAAGATGGTATTCTTTTAACTAAGTTTTCTATATGTTCCTTTTATTCTAAGAACTTAAATTTTCTTGGACCAGCAGTCATTTTCTCTATTTTTTACTTCCATTTCTTCCGATTAGTCTGCCCTCCCTCCTTCCACTTTTTCACATCCTTGCAATCCCTTTATTGTCTAGACTTTCTACGGAATGTTTAGCTGCAAAAGACCAGAGAATTATCAAAGGAAATTTTGGGTTCTTCCCAGTTTAAAGGCCATTTGCATAGCCAACTGGATGAAGGTAAAAGCAGCTGCAGGGTATTGATTCGACATGAAAGCCTTGAGTACACGTTCCACTCTGTGTGTCATCTTTTGCGACAAATTTGGAATATACCTTAACATTTCAAAGGTAAATGTCAGCCGGAGACCGTGCACCTGCAACTGACATGGTCGTGTATATTTGCGTATATATACGTACATATATGTATGTACATATGTGTGGTACCTTCATCTGGGGCCCGGGGCACGGTCAAATGGGCGGCTTGAGTGGGTGGCACGACATTAAATGTACAATATGCTCATTGCAATCCCTTCAACCTCCCCTCGTGGCCAGCACTCGCATTTGATTTGATAATAACGTTTTCGAAAATGTCGTTTTCTCCCTACCACAAATTGATTTGACTGCCTAATCTTGGCTCTTTTTGCTCCATTTTGCCACGTG
 

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