CR01012

CRIMIC CR01012

Gene Tomosyn

CG17762

Information

Donor Location Cytology FBti Stock number
pM37-p2X:12637023..1263702311B6FBti020693783249

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK ACAACAAGGACGAACTCAGACCAGCA CCTGGGTCTCTGTAAAGGCAGACAAA

Images

Construct Design Sequences

sgRNA sequence GATGGAGATTATGTCATCGGGGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
CCCCATCGCATAACTGTGGA GAGATGGAGATTATGTCATCGGG TCCATCTCTCTTGCAGCGTG GCGGCGGGCAAATTAAAAGA
 
Amplicon 5' Amplicon 3'
CCCCATCGCATAACTGTGGATAGCAGAGCAGCCGGAGCACGAGGACGACTCATGTTACAAAAATGAAAAAGTTCACATTCAAAGGAGTTCTAGATGGATTTCGACAAAGCGTTCAGCCCCAAGCCACACGACAGGAGCAGGAGATTCAGGAGCAACTCAAGGCCGATCACTTTACGTTAAAGAAAGTAAGTTAAAAACATACGATAAATGGGATACGAGTTGAAAGTGCCCATAATCGAAAGGCATCTGTTGCAGCTAGCGGGTGTTTTATTTGGTAGCTGTAATGATTTGAATCTATCATAAGACACACGCGGCTATAACCGTTGTCTAAGAGCTTCAAAATCGATAATAACAAACAATAAAAGTAGTAATTTATAATACTAAATCATACGAGTTAGCGTGATTAGTACGTTTGATCCAATTTCTTCTCTTTATCGCAGACCTTTCGCCATGGCTTTCCATACTCGCCCACATCCTTCGCCTTCGATCCAGTACAAAAGCTACTAGCGATTGGCGACAAATCGGGCTACATTCGCATGTAAGTGTGACTTGAATACAACGTTTTTCTCATTATTTATTAATCTCATCTTTCTATATATGAACTAATTGCAGTTTGGGACGACCTGGCGTCGATGCCCATGCCAAACACGAGGGCGAATCCGAGTGCGCCGTGCTCTTTGCCCAGTTCCTGGTCAACGAGGGCGCATTGGTCACCGTCACCGCCGATGACACCATTCACTTGTGGAGCATTCGCCAAAAGACACCGCGCATTGTGCAGAGTCTGAAGTTTCAGAGGGAGCGGTAAGTTCAAGTCCATTATGTTTGGTCTTATTATGGATATCACGATATCGTAATACTGCCGTCCATAAAACGGGTTATCAATTTGGCTAATTCTCAACATATTCCAACATATGTATATCCCCCGATGACATAATCTCCATCTC TCCATCTCTCTTGCAGCGTGACTTGCATACACCTGCCTGTGGGCAGCAAATGGCTGTACGTGGGCACCGAGAAGGGAAACATACACGTTGTTCACATAGATACATTTGCACTTAGTGGTTATATTATTAACTGGAATAAAGCAATTGAAGTGTAAGTCCAATTACTCCCTTAGTGACCTAAACCGACCGCATTAAGTTCTAATCACGGGCTTAAACCAATTTGCAGGGTTAGAACTAGTCATCCAGGTGCTGTGATTGCGTTATGTGATAATCCATTGGATGCAAACAAGGTGAATACTTTGTTTCAATGAGGAAAATGCCTCAAGAAGATTTCACTCCCCTTAAACTTAATGCATATATTATATTATTATATTCGATGACTGATGAGATTCTTTCCGCACAGTTGCTTATTGCATTTGAGTGCGGGCTGCTTGTGCTGTGGGATCTGAAGGCGAAATGCGCTGAGCTGCGATGGCAGGCGGCCGAGGCCGTCAAGTCCCTGGCCTGGCACTACGAGGGCAAATATTTTGTGTCCTCGCACACGGATGGCTCCATTTGCTCCTGGCCCACGAAGCCGCAGGCTAAGCCGCAGTCGCAGGTCTGTCCGCATGGTAAGTTGCACAGTCATTATCCATATATAAATATATTTGATTCGATTTATGTATGCTTTAGCTAAAATCAACAAGGATGGCAATGCGGAGAAATGCAAGCCGATATATAAAGTTGACTTGAAGTCCAGCGCAACTGGGTGAGTAATAAGCACTGTCGAATCATTTGTTTACTACTATGCTTTATTTATATTACACGGAGATAGCTGAACAACCTAGGGCGTTATCTTTATTTTTGGAACAATAACCATAAAGCAATTTTACAATAATATTTCAACAATAAGAATCATTGCGCTGCCGGCGTGTCGATAAAACAAATGAATAATGATAACTTGACTCATTAAAAATTTATAATCTTTTAATTTGCCCGCCGC
 

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