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CRIMIC CR01006

Gene Fatp1

CG46149

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:10516482..10516460	31F5		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAGTCTCAAATTGATGGCGTCCTTGC	ACACAAGTCAAAAGGCGACACTTCCG

Images

Construct Design Sequences

sgRNA sequence	AGTGCTGCTCTTGTACAGGGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGTGGCAACCCTGGAATCTT	GCTGCTCTTGTACAGGGAGG	GCACTCACGTTAGATCGCGA	GGTCTTCCCATCCAGCACAA

 

Amplicon 5'

Amplicon 3'

CGTGGCAACCCTGGAATCTTAACACTTCCACTGTAGCCATAAAAAAGTGAATCACTTAAAAATTAAAAATCTATGTGGTTTATAATATTATTGTAATTTCTTTAAATTTATACTATTTATGAGGTTAGCAAACTTACTTTGATATCCAAGTCCTAGACATTCTTAACTATAACAATTTTAAAAGTAAAGAGCTGCTTAAAAATTCTGAATACTTAATAACCTTTCTGCAAAATTTCAAAATGTAAGTAGCCCTGGCAACCCTGGAAAAACGAAAATTCCAAGACGCAGGCGACACTTCCGCCATCATAATGACCCAAGCTTTGGCTCTTTGGCGTACGTTTTCAAGAACAGATCGGTTAGTGTGCCAGTGGTCTTCCGCCTGACGCACTCGATCGCATTCGGATCGATTAGTATGCGCGGAGGGTTTCGCTGGGCGACGATCGTATGAAGTCAATGGTAAATAAAAGCGAGTTTTGTTTTGGTTTCTCGTAGGTTTTTTTTTTTTTTTGGGCTGTGCGACCTTGAACCTGAAGAAGTCAATAAACTGCTCGTAATCGAGTGGCAAAAACTAATAATTTGCGTTGACTATTCGTCACAATGTCGCATGGATCATTTCGCCAGTTAAGTTTTCTCTTTTATGGTTTTCCCAACTGGAAATGAATGCTGCTGTTGGCGACCCAAATAGATGGAAAACGAGTTTTTTCCACTGTGGTTTCTGACTTTCGGCTACGAAGTGAACCTCGCACTCGGCTGATTTTTCAGTTCGCTTATGTTCTCAATCGATTCACAAAAGAAAAGCCCCCAAGATGTTGTTGTGTATTGAAATGCAAACCGCACGGGGGCGGTATTGTAAACTTATCACTGGACTTGGTCTTGGGTACAGTATGCCCTCCCTGTACAAGAGCAGC

GCACTCACGTTAGATCGCGACTAGCGGTCGCACCCGCTATGTAGAACCAGCGCCAGCCGGGTTTCGTCAGTAGCAGAGCCACCAGTGCGACGAGCACAGCAAAAATCCAGCCCATTGTGTGGAGCGTGTAGATGACGCAGGCGATTGCCAAACCGAAAGCGACCGTTGCGCAGAAAAACCGAAAAATGACTAAAAACCGCTGGCGGCGACGTCGCTGTCGATCGTGCGAGCCGTCGGTTATCGACTTTCGATCAGCTGGCCGCTTGGCGTTGCCAGATCAGCGAAAAGAAAATAAGATCGTTAGTATTCGAAATGGGTTTTGAGATTTAAATTCTTACATTGGTGCTGTAGAGCGCGCAGTTGTCAATGTTTATTATCACATTGCTGTTCGCATAGTTGTCCAACAGGCTGTTGTGCTGCAGAAACATTTTTGCATTTTATTTCAGTTTTTAAAATTTGAGTGTTCTTTGTATTGCATGACCACTGTCTTGCTCCATATGTTGCACACATTACTTACTGTAAGCTCATGCCTCGGATGACCAATATTTGAGCTATTGTAACGCAAACGCTGATATCGTTCGTTGCGCTGTCACAAGAATCCATTGAACATGTAGAACGGCAGAAAGTGTCGGCGAGCCTTGCGAAATAACATTTTCCGGTATTTAAATGGGAGTAACTTTGCAGTTTTTAAACAATTGCCGTTGCTTGTTTTGGTTTTGTAACTTGTCCTGCGCCAATTGTTTTTTTTTCGAGAGCTTTTGATGCTGCTCTTAGAAACTAGTTCCTAAAATCCAAAACATCGGTATGTGGTCCACCACTAACTATCGAATGGCTGCTCGATATCATCAGCTGTCCCAACCATCAGGTGGCAACTCTTGAGGCCAAAACAAATGTTTAGTTTTATTTAGCGGGTTTTTTTTTTGTTTATTGTCTAATCACTGCCTCGCCATGAAGATTTTGTGCGAAGTTCAGGTGGTGAATCGCGCCACCCAGACGAACAAAACGCCACGTCCCGTGAAATCCACACTGGCCATTGGCTACAAGCAAAGCGCCAGGGATGCGAATGGAAACACCAAGAAGGAGCTGGAAATGGTGCTCTTTAGTGGACAAAACAAGACTGGAAATCGCTACAAGGTCAAGGATAACATTCATGTCGTGCACACCAAGTTTGTGCTGGATGGGAAGACC

 

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