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CRIMIC CR00955

Gene Lac

CG12369

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:12460412..12460434	49A7		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TAAAAGGAAGTCACAGGCGGGTTGGT	GCTATGCGAGGCAAACAAAAAACCGG

Images

Construct Design Sequences

sgRNA sequence	AGAACTCGGCGGTTCATATAGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CACATTGTTGACGTGCTGCA	CCGCCGAGTTCTGAAAGTCT	AGGGACAGTCATGCTAGAACC	GATGCTGCGTCCATCTCTGA

 

Amplicon 5'

Amplicon 3'

CACATTGTTGACGTGCTGCATTAACATTCATAATTTCCACATGGCAACGTTCGGTTCATGGAGTCAGTTGACATTTGAAGGGCCCTGCAAGAGAACGAGATAGACAGGCTTTTCCTGAATTTTCCTTAGACTTTCCGCCGGAGTTGCTGGCCAAATTGCAGGAAATTGAATGGGGAAAGAACAAATATGGCCGCGGGCCACGACTTGCCGCGGAAAGGGAAAGCGCCTTGCATCAAATGTTATTTTGGCTAGTTGTTGTTGTTGCCCGTCTCACACGTTTTCCCGCTCGGCGGGTACAAAAAAGGGGGTTTGAAAAAAAAAAACAGCGTCTGGCCCTGTGATTGGTGCGGATATTTTTTGCATGCCACTCAATTTCCCTTTTTCCAGCCCTTTTGGGTGGTTGTTTCTGTCACCTCACAAAATGGCGCTCAACGGCGGGGTTTTATTTTGTCGCTTTAAGTAGTTTATGAAACATTAGAAACGGTAGCAGAACCAGCAACCCTTTCCACCATCCCTACTTGTCACCAATTTTCCAAGCGAGCGCTCATTGAAAGTCATCCAAGCAGGAAGCCAGGAGACCGAGAGACTTTCAGAACTCGGCGG

AGGGACAGTCATGCTAGAACCTACACCTACACCTGTTCCGTGCATTTTCCATTTTCCATTTTCCGTTTTCTGTGCAGCGTCTCAGCGAAGTTGCATCCTTTTAGGACCTATCTACCTGCACTTCTCACATTTTTCCCTGACCAAAAAAAACTGAATTCTCACCTCTAGCTTTGCACAATTTTCCATTTCTTTGGCTCTTTTTTCGTTGAGCCTTCAACTGACAATTTATCTCTGGTTGTTTGTCCAGGGAGCAGAGGGATCCGGAGTCGGCAGTCGGGGTTCCAGAAACAAGGGGTGTTGCGTTAGCAACGTTGTTTTCATTTCGCATGATGGCAGGCGGAGACTTCCAGACAGGTTTCCCTGAAGGAAGGGCCTAAAACAAATGTGGACCCGCCCGCCTGCCAAGTCGGCGTTTTCATTACCTTTTGAGTTTCCATCTTTCCATCTTTCCTGTTTCTGTGCCGTTGCTGTTTCTGTTTTCCTTTCATTAGTTGTCAGAGATTGCCGAGTCAGAGATGGACGCAGCATC

 

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