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CRIMIC CR00951

Gene Nak

CG10637

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:18967417..18967439	37B7		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCACTGCATTGTGTGTGTGCATGCAA	GCGGTGGGGCGGCAAAATAAAAAGAT

Images

Construct Design Sequences

sgRNA sequence	AGCACAGGCGATTTCCTTAGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCGAAACGCAAACGAGGGTA	CTGTGCTGTGTCCTTGTCCT	GCCATTTAGCTCGGCGAAAG	TCGCCCGCGATTATGCTTAT

 

Amplicon 5'

Amplicon 3'

TCGAAACGCAAACGAGGGTAGAAAATACGTCTGAAGGGGCGTCAACTGCAAGGTGGGCAGTAAGCGGTTAAGGGTGGGGGCTGAGGGGTTAAGGCGGGAAAGCTTCATTCGTTAACCGTTTGTCGCTTAGTCATTTGTAATTGTTGTTGGCTGCTCAAAAATCGAATCAAAGAAAGCAGGTAAATATTGAAGGTGATTAAGCACAGTGTTACTTTGGAAGTATCCAGCATATCGAGCAGTTTAAACTTGGCTATACGCGCATTTTATTCATTGAGCATCTGCAAAAGACTCTATTTATTTTTTCTCTTTCTTAAATGAAATACCAATAGTTACGTTACTGGATTAAATGAATTAATAAATAATATATAATTTAGTTTATAATTTGTTATACTAATTCAATAACTTTAACCAAATAACCGTGTTCGTCAAACGCTCCGCCTTTTTAATTACAGGGTATGCCCGACAACAGCTGACTAATTGCCAGCGGCGCCAGCGCAGCGCAAAACAAAATTACGAATCGAGTCCGCTTGCCAAAAGTATAAAAAGTAAAAAACACTACGTAATGGCTAAAGGAAAGGTGGCGAATGGCTGGCCAGGCGGTGGGTGAGCAATCGTTAGATGATCAAATGTTGGGCGGAGGGTACTGGTGCCAGGCCAACAAGGAGGCACTTGCACTGCAGGATGATGTGGAGTGGAGCACTCCGCATTTCAAGGACAAGGACACAGCACAG

GCCATTTAGCTCGGCGAAAGGTGAAAAACGGAAGACTGTCGGAGACAGTCGGCTGATGAGTGTTGGTCTTGCCGCTTCATTGGCTAAATTAACATAATTGATTCAATTGATTTGTGGCCATGTGTAGTGGCTACGGGAATTCAATGGGTATTCCATGGAAAAACCAACCAGAACCGTAACTGTGTTTGTACAGTCGGGTTTCTCTAACGCGGACACCCATTTGTCACAATAGAAATGCTGGCATTTCTGGTTTTGGCAAAAATAAACTCTTTAAAGACTGACATAAATTTGGACGAAATATCTGCACGCCATTAGAGAGCCGAGTTATGGACATAAATATGTAGTTCTATGCCAAGGGCAAAATTGCAGTTCAATTAGTTGGAATTTAGGTTACAAACCAGTATTTAGTATTCATAATTAATGAAATAATTTAAAATGTTTTACAGTTTTATTAAAAGATAATATATCAAAGTCAGATTTTAGCTGTTTTGAGTTTAACATCTTATACAGAAATTATTGGGAGTAATTTATTTGGATTTAATCGGAAATGCGACAAGTGGTGTCCCCAATTATGGAGGATACGCAGCTGATTTTTATGCTCTCTAGCGCTTTGAGTACGCCCCACATTTGGGAGTTCTCGCGCTGAAGGGGTTTATCTAATCTGTGACGAGGGGCGAGACCGAAAAGCGGACGGCAGGCTACTGCCCACCTGTAAATCAGGCAGGTGATAAGCATAATCGCGGGCGA

 

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