CR00890

CRIMIC CR00890

Gene Inos

CG11143

Information

Donor Location Cytology FBti Stock number
pM37-p02R:7458002..745802043C3FBti020684383197

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK short band TGTACTACGATCTCCGGCTGGAAAGA GTGATTAGAGCCGATAAGCAGGAGCA

Images

Construct Design Sequences

sgRNA sequence ATTCCGGGGAGTGGTCCCTATGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
TTGGGAACGTGTTGGTCCAA CTCCCCGGAATCGCAAAAGA GAGAATTCGGCACAGCTCGG CGGAATAAGAGAGTGCGCCT
 
Amplicon 5' Amplicon 3'
TTGGGAACGTGTTGGTCCAAAGTCCGATAACAAGATGTCCATTTGCCGGCCTGCGAAATTATCACATTTCTTCAGCCGGCTAAGAGAGGAGCTCTTTCTCTCTTTACATTAGATGCTCTCTTCGCGGGCATCGCCTGACAGTGACCTTGAGGCGCAGCAAGTGTCCGTGGGAGGGAAGTGGGCGAAGGGAGATGGTTGATGGGTGGAGTGTATTGTTTGCAGGCGGTGTAAACAGAGCACGTACCGCGACCACAATCACTTGGAAAAATGGGCTATTGGGTTTCTAGTCGGGTGCACCAGCCCCATTCCTATTTATTTAGGTGCCATTCGAGCGGCCGAAAAGGCGCCTGCGCATAAAATCACGCCACTCGCGCTCGGTCCGGAATAGTGACGAGATATCTATCGGCCGTATTTGTCGTCAGCTGTTCAGCTGAGCAATCAATAAATTGTAACTGTAACGCCCCGCCTGTGTGAGTGCGATTGGATAGGAGGCCGGCCCTCTCTCTTATCGCTATTTTTGTCTTTTGCGATTCCGGGGAG GAGAATTCGGCACAGCTCGGTCCTTTGCTGGATAATGGAAAGTTTCACTGGGTCAATTTAGTTGCTAAGTTAACGCTTATATTGGGGTAGCAAGTTTCAGTTCATTACGCTATAACGAGCTAACTCTTAGTTAAGATTTTAAACTAATTGTGCTTGGATGTGATACGTTAAGGATGTTCACAATTTAATTTATAGTACGAAATTAATCAAATAACTTTAACATGGTTAAATGGCCTTTTCATTAAGTTCATAATTTAAACATTGCTTGTTACAGATTTATATTAAAATTTATAAGATTCTGTTTTGTAAAATAGAATGCAATTAGTCATAGGTGGCCTATGCTGGACTCGAAACAAAATAAACCACTATTTCTTCATCCAGGTGAGAAATATTTTTCTCCGAGTGCAGGCGAGTGCGCATTTGAGCGGGAGAGCTTATCCATCCCGCCTGTTGGAAAAGTTCAATGTGAAGGTCAGCCGGAAAATGACTGTTCGCACCACATTCGATATCAATATCATTCAAGTGGATGATGCTTCCGATCAACTAGTGCCCATGGTTCGATAAGCAATCCCTTGACGATTGGACCTACCTGGAGCTGTCCATCGGCGGTGCGCTTCACATGGGATGTCTGGTAATCGTAGTCGGTGGTGATGAACTCATCGTCCACCTGCACTTTCGGCGAGATTACCTCCAGGGTTGAGTTATTGGTGGGCTTCATTCTGGGATGTTTTTGCTAACACACTCTCGTCTTGTCAATTAGCGCGTTTCAATGTGCTTGCTTCTGGAATTCCTGGCTTCTGCAAAAGAGACTGCGATGTGGTCGAGGCAAAAGTTCGCGGCGAATGACTCGGCTGAGTGGAAATCGGGCCGCTTTTATGGTCTCCCGCGGGCAGAGGCGCACTCTCTTATTCCG
 

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