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CRIMIC CR00813

Gene Rgk2

CG34390

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:18545813..18545831	55D3	FBti0200697	80675

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAACCTAAGTGCGGCTGCAGGATCAA	GCCCCGCAGCCGCTTTTGATAAAAAT

Images

Construct Design Sequences

sgRNA sequence	CACACATAGGTTCCAGCTAGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CATGTGGAGCATAAACCGCG	TAGGGGTCACTCGGCTACTC	TACCTGACCCCTAGCTTCCC	CACATGCAGATTCTCGCAGC

 

Amplicon 5'

Amplicon 3'

CATGTGGAGCATAAACCGCGACGCCGGGGCAGCTCCAAAGGTGGACTGGCCTACTTGGCCAGCCGGCGAAGTTCCCGTGAGTCGATGAAGAGCGCCTGCTCCAATGCCTCCATCGTTTCCAACGACGATGTTGGACCACTGGCCTTCCAGAGTTCGAATCGCGGTCGCCAGCGTAGAACATCGAACTTTCTCGAGTTGCCAGGTGAGAATGATTTTGTTTTTCATTGTCTGTCTATGATTATTCTTAATTTTCTTTTTTTTCCTCGTCTCAGTTCCCGACCATGTGCGTCCGCGAGTCTGTTCCCTTCCAGAGCGACCGTACAATCCGCGTGCCAGTGATGACCTGTACCGCCTGCGTCACTTCTCCATCAGCAAGGGCAACGTGGTTAACTGTGGCGACTCGATAATCTCGCGGAGATCGCGAAGCAACACCAGCGTCAACTCGACAAACAGCCGGGCCAGCGAACGTTCCCCATTCGAGGGCAGCTGTTGCGGAGCGGGCTATGCCAATGTGGATTCGCTGCCGGCAACACCGGATGACTCGGAGAATTTGGATCCACCTCCGCCCGCCCGCTACCGCGTCGTGATGCTGGGCGATGCCGGCGTGGGCAAGACGGCGCTGGTCAATCAGTTCATGACGTCGGAGTACATGCACACCTATGACGCCAGCCTGGGTAAGTTGTATGCCAAAGTGGGTCCGGGTTATGGTCCGGGTACCTCGGGGACGATCCGCAGAGCGCAGTGAGTAGCCGAGTGACCCCTA

TACCTGACCCCTAGCTTCCCGGCTGCCACTGTCGTCTTTAGAGTTTAATTCTGATTTGAGTTTTAGACGACGAGTTCGGCGAGAAGACGGTGAGCGTGCTGCTGGACGATGAGGAGTCGGACATGGTCTTCATCGACCATCCAAGCGTTGAGATGAGCGTGGAGAACTCCCTATCCACATATGAGCCACATGGCTGCGTCGTGGTCTTCTCGGTGGTGGATCGCAGCTCCTTCCGCGTGGCCGAGGAAATCATAAATTACCTGTGGCAGGAGAACTACACCAAGGACAAGGCTGTCATACTGGTGGGCAACAAGGCGGATCTGGCCCGCGCCCGGCTTATCACATCACAGGGTAAGTCCGGGGGGTCTTAATTATGATGGCTTGATTTCATTTCATTATTCCCCAATTCACTCTTGCTTCCCCAAGAGGGCAAAGCGCTGGCCGCCTCACGCGATGCCAAATTTATTGAGACGTCTAGCGGCATACAGCACAACGTCGATGAGCTGCTCGTCGGCATATTGAAGCAGGTGAGTCGGAGGTGAAAAGCTAGGACAAGCGGACCATTGGGGGGCAGAGAGGAAGTGGGGCAGTGGGGCTGCGGTCGTTGGGGCAAGGTGCCAACCACACGCAACCCACCGATGCATGGCGAAATTGTGCAGGAAATGGAGTTAAATGGATTTACCACGCTGCTGCGGGTTGGGTTGGTTTTCGGGTTGGGTTACTGCTTCCGGATCGGCTCACTAATTAATGAAAATGGTGCGGTAAGCGGGACCGAAGGAAACTTGGTCAGCAAACTTGATTAGGTGATTGGGTATTGTTACTTAACTCGGAGTAAGATATTTCCGATACTGGTAGACAAGGTTGTATATAAAAAATAATAAAGGGATGTCTTGATTACACATAAAATACATTTTGACATTTGAAAACTTAATCTAAGGTCTACAAATATTGCTGTTTTGAGGAAGATGCCTTTAAGAATATTTCTTTATACCATTTTTCATTCTTACACTATAAAACAGATGCGGCTGAAAGAGAAGCGGGAGAAAAAGGCCACCGCCTCGAAGATGAAGACTTCCCGCACCCACATATCGCTGCACTTGGCCAAGGAACTGCTGCAGAAGATCTGCCTCAGCGACATCTCCAAGTCGAAGAGCTGCGAGAATCTGCATGTG

 

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