CR00649

CRIMIC CR00649

Gene CG4945

CG4945

Information

Donor Location Cytology FBti Stock number
pM37-p02R:16567177..1656717753C8FBti020061783182

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK short band AATACCAGAACCGAGAGCAGTTGGCA AGTCCGAGCGATAGATGAGCACATTG

Images

Construct Design Sequences

sgRNA sequence TTTCCCGGGGAAGAGACAATTGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
AGAGGCAAGCGGGCATTATT CGATTTCCCGGGGAAGAGAC GTCTCTTCCCCGGGAAATCG CACGTCGTAGGTGGTCACAA
 
Amplicon 5' Amplicon 3'
AGAGGCAAGCGGGCATTATTTTTAATTTAGCTGTTGATATTATGCATAAAGCGAAGAGAGAAAACAAACATCAAAACAGAATAGAATCAGCGAAGCCGGGAGAGTGCCGAGAAGAATTCTACGGCCTGGTTCTGAAATCTTTTTTCCTCAAATTCCGACCGTGTGTGTACGTGTCAAAATATTTATACGCGCACACGCTCCACGGATTATTTGTTTGGTGAATTTCCACCAGGCACAGTCTGCATATATCTATCTACATATGTCCGTTTACCAGGAAGCGAATATACAAATCCCAATTCAGCCGATCAATTAATCTCTTAATTTACTTTAGCCGCGTGCAGGAACAGCAACAAAAGATACATCCATTAATGCAGATACTAGCGCGTATAAAATAAAGGAATCGCACAGATATAGAAATGTTTTCCTTTGGTGTTTCTGCTGCTGTTTCTGCTGCTGTCGCTATAAATATTTACAACGCCCGCCTTGTATACAAAGTGTAAATATGTAGATACAATCTGCTAGATACATTCGCGTACATAAACTGTTTCAATTGTTTTATTGGCCCGCTCATGGACAAAGCTGCGCAGAATTGAAAGTGAATCAAGTGCTATGAACCAGTCCAGTAAATAAAAAACTGACTTGCAACCACATAAACAGCCAAAATGTCTAAGAAACCACGTGGAAACATTCACAAGATACGAGAGTTCGAATTGGAGAAGGTGAGTGATCGGATATGATTCAATCCGAAATCGAATTGTAATACTCATTCATTTCCGCGCGACTTGTGACCCATTATTTTTATTTTTTTTATGGTTTACCGCGCCGCCATAAACAAATATGCAGATAGAGAAAGGTATTAAGAGTTCGTCTTGGCCCAGTCGGTATGATGGGCAATCTGCCAATTGTCTCTTCCCCGGGAAATCG GTCTCTTCCCCGGGAAATCGTCTACAATACAACTGGGAAAATGCCATGTTAACGATTAGATTTCTGATCATTAAACACAAACAGCAATGGTCAGCGTCTCGTTCTCGAATACAGTAATGTTTAATAGGTCTAGGTAACGCATTCTATACATACATATATGTAGTATATAGTTGCCCAGTGCATTTGCACATTTAATTGTTCAGATATAGTTGAGGTGGGTCTTTAACGAGGCGTTGCACTTCGCAAGAAGCTCTCTTTGATTTTTTCGCATAAAAGGCAAATGTATTAGTGAGTCAATTAGTTCGTTAATAATGGAATTCTGTTTATGAACACGTGTTGCATTCTAAATCTAAATGAAAACAGCAAGTGGTGCAAATCAAGTATATATACTTTGAGTTCCAGAACATGAGGTTCAAACTTTTTAAAGGCATTAACTTTGGTGTAACTTCTACATAACCTATTTTTAGTTTAAGTTTTAACAAAATATGTGCCCACCAATATTCTCTATTTCAATTTAAAGATATTATGGTTCCATGAAGTTTTTCTATCCCCATTGAATACATCTATGCATATATCGCAGCTGTTTTAATATGCCATCTATATATAGATCCAGCTTGTGGACGAGTTTGATATCATACAGATCGTTGGCGAAGGATGGTTCGGGAAGATTCTACTGGTGGAGCATCGGGGATCACAGACGGAGATGGTCCTGAAAGCTGTACCCAAACCATATGTGACATTGCGTGACTTTTACCGGGAATTCCACTATGGACTCCACTTGGGAGTCCATCGACACATTGTGACCACCTACGACGTG
 

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