CR00557

CRIMIC CR00557

Gene Su(z)12

CG8013

Information

Donor Location Cytology FBti Stock number
pM37-p13L:19919650..1991966376D4FBti0200584Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
no band short band GCACTGAGATTGTGCGCAAGAATCTC AACCGGATGTGAAGTGTAAGCATGGG

Images

Construct Design Sequences

sgRNA sequence TTGTGAACTCATGACGATGGTGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GGCGTTGTTACTGGTTGCTG CCGATCAGAAGCAAATCCCA TGAACTCATGACGATGGTGGG GCTGAGCTCCAAAGGTGAGT
 
Amplicon 5' Amplicon 3'
GGCGTTGTTACTGGTTGCTGGCAGTGAATGGTTATTGCTTTTGTTGCGGTTGGCTCCGCTTCCACTTCCGCCTCCGTGTCCATTTCCGATCCCGGTCCCGGGACTTCCGTCGCTGTAACGCTGCCGCTTGTTAAGCACTGTATTATCTAGCAAAGGTCCAAAACATATACCATTAGAATTAGAGCGGGTCAAATGCAAGTTGAATCCAAGTTACTACTGATTTAGTTGCCCACACAGTTGTTGGCAACTGGGCTCGCAACTCCATTCATAACTCCCACGGCACCAAATCCGCCACACTCATCATCAATAATACCAACATCGACAGCATTATCGCTGTGGGTCAGCACTTCTGGCGCATCCGCCGCCTGCTCCACATCTGCCACAGACCGTGCGTTCACATTGAGAGGAAGAATTCAGTTAATGTCCAAAGGAAAAGCGAAAGTTTTGAAAGAAAAAGGTCCAAACGATACAATTTTGATTGAATTTTGGGATTTGCTTCTGATCGG TGAACTCATGACGATGGTGGGATGCATTGGATGTTTTGTACATATGAAGATGCATGTAGCGATTCCTTGTCTTGGATATGTTATCAGTTTGGTCTGCATAAAGCTACTTTATGTGGCACAATTGATGATGATATGTTCAATGGGGGAAATAGGGGCTTACACAAATTGCAAACGATAAAAGATTATAAAAGATAATAAGACTTATCTCAAATATCTCAAATAATAACTTCATTTACATCGATTTTAAATGAAATGAGCAATTCGGATTGCCAAATGAGAAAGTAATTCTTTGATTTTGTATATATAAGGCTCACACTATCGAGTTGCAATTATGTTTAAGTCTCAGAGACGATTCATTTTTCATTAGGCATCAAGTTTAGTCAGATTACTGTTTGTAATCCCAAGCCATTGTAAAAACAAAAAGGCAAAGTTAAAATGACTACGATGGGGATGAGACGGGTATGCTTCCTGCCTGCTGATGCGGCACTCACCTTTGGAGCTCAGC
 

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