CR00451

CRIMIC CR00451

Gene Sox21a

CG7345

Information

Donor Location Cytology FBti Stock number
pM37-p23L:14105355..1410537770D2FBti020055983174

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK no band TCACCGAAGGACAGAAGCGTCCATT TTGGCGGTGACTCCTTACAGATTGCA

Images

Construct Design Sequences

sgRNA sequence GCTTAAACTAATGGCCATTCGGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GGTGCTCATCCTAGACCACG GGGGTTTTCGAGCGAGCATA CCTTCTGACGAGCCCTGATC GGCAACACGTCGCATGAAAT
 
Amplicon 5' Amplicon 3'
GGTGCTCATCCTAGACCACGTGTTCCGCGGATGAGGAGGTGCCGGCGCCGAAGCGCAATGGAGTCGGTCAAATGATGTTTGGCGGACTGGCCGCGCTGGCCACTACCGCATTTGGTGAGGACTTCTCTGACTTGACGCATCCGTAGGATGAGATGCCGGTACCGCTGCTGCTGGGCGGGGAGGGTGGAGTTTCCGGACTTATCAGCGGCAGATATCGCCACGGGTGGTAGATGGAGCCGGCGTACATCGCCTGGAGGCGCGCCTGTAGGTCCAGGGCCAACGTGGGATCCAGTGGATAGTGGGCGAGGCCGGGAAAGGCATTCGGCAGGCGGGGCAGCGATAACTCCAGTGGCGAACCAAAACCGTACTTGGCGCTCAAGGTGGCGGCGGCCAGCTGGACATGATGTGGATGAGGATGCGGGTGGTGTGGATGCGATTGATATGGTGACTGGTGCGGATGTCCGTGGAGATGTGGCAGCGACTGACCCAAGGGCGACAACTGGGGCTGCATATCCTTGGGGGAGCCAACTGATCCTGGGCCACTGGGACCGGAGTTACCCGCACCACCAGCGTTGACACCACCGTTGGCACCACCTCCACCGCCGCCACCACCACCACCTGGCACCGGTGACTTGTTGAGCGTCTTGGGCTTGCGACGTGGTCGATACTTGTAGTCGGGATGTTCTTTCATGTGCAGAGCCCTGTAAATTAAATAAATGTTGAAACACTGATCAAAAGATCGAACGATTTAAGCTTTGATGATATCATAAAGTCAGCCATCTCTGGTCGTAAACCCCAAGAGAAGCGGAACCCTTCCATAAACCCCACGCCAGGCAGATACAAAATGCACAAGTTTATATTGTGTATTTAGCGAAGAACCAGCACTCCGATTCCAACTTCGATTCTCTTTCCGATTTCCCAGCTGCAACAATGCGCAATTGTTGACATTTGTTTGACATAACGATAAAACCTGATAAAAGGTGTGTTTGGGTCGCCTCCTCTTGTTTTACTTTCGCTTTTCTTTATTTTGTTTTCCTTTCTTTGGTCAGTTATGGCCTCCTTATCGCGGGTCCCGAAAAAAAATATTGTTTTCCTTATCATCGCGCATTGGCAAATATTTGATCAGAATTTTTGGTTCTTTTCATTCGATATGCTCGCTCGAAAACCCC CCTTCTGACGAGCCCTGATCATTTATAAATGTCATAAAATGTAAGTAAAATTTAACATTTCCATTTTGGCAGGCCAATCCAATCAATAAAAGGATTATGACAGTGCAAACACAGAGGTTCGTCATCATAAAAGACCGCACTATCTAGGAAGGCATAGAAAAAACGAACTTGTGAGATACGGCATTTCTGGGAATTCCTGTGGTTTAAAAGTGCACTGCAAATTTTGATTCCATTTATACCAATACAACTTATTTAAAAGCTAGTTTTATAAAAAATAAAATAATGTGAGATATCACTTTTGTACTTATTTGTAAAATGCTCGCTTTTGAGTATATTTCTTGTACAAAGAACTCCATTCTAGCGGTATTTTCAGTTAATCCCGATAATTACGTTTATTAAAACTAAGCTCAAACTGAAATTACTCAAACCCTTTCGAAAGGGTACACTGAATGGGTTAAGCACACAAAAAGAAGCAAAATGCAAAATGAATGGAGCATTTCATGCGACGTGTTGCC
 

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