CR00327

CRIMIC CR00327

Gene BubR1

CG7838

Information

Donor Location Cytology FBti Stock number
pM37-p02R:5973328..597333542A6Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
short band no band GGTCAGTGATGACGTTGTGTTGCTAAGC GGTAATAGTGGATTTAGTGGTGGTACCGC

Images

Construct Design Sequences

sgRNA sequence TAAGGTGCACCTGATAGTCAAGG
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
CCTCCTGCACAGACGAAACT TCAGGTGCACCTTACCTTAATT AGGAAACCGACTCGTTCTCT GACTGTCCTACACCAGTGCC
 
Amplicon 5' Amplicon 3'
CCTCCTGCACAGACGAAACTCTGCGTGGCATGATGGATTTTCTGAAGGAAGAGGGAAAGAAGCTCTTGCGGCGTGGCATCTTGTTTCCGGGCAAGCAAACGGTTTCTTCCATGTCAAAGTCACCATGAACTGTCACATTTCGTTTTTCCATCCATTTAGCTGCCAACAACTCCTCTGGCTGGTACTCCTTTTGCTCAACTTCGTTGTAAATTTTGTCAAAAACTACCTGAAGGCCCGGTACCAGTGGGGGTTTCCAAAACTTGTCTAGCTCATCGAGATCTTGTGGCTTAGAATATCTGGCGAAATTCGGATAGCAGCGAATTCCTCTGATCACCCGTCCAGTGCCCCACCAATCATCATTGCGATGGATAAATGGATGCTGTGGGTTGCGATGCTTCAAAAAGGAATAGGCTCGATACTCTTCGGGCGAGAACTCGATGTTGGGGCGGGGGTAGAGGAGACACTTGTTATAGCAAGGCAGTCCACTTGCGTTTGGCTCGTCCTCAATAGTAACTGGCGTCACCCACGGTTCCTGAGGCCTGTTCTTGGCCACAAAGTTTGTCGGAAATCTAAATGGTTGTTCTAGACGACGCTCGTAGTTTGTAATAGGTGGCATTGCTTGTTCGTCAACGTGTATATCAAAGCCCAGATCCGGCGAGGCGTTACTGCCGAAAATTTTTCCATGTTTGTGGCGTTTAGCATTTGCCTTATTCCAGGCCACCGGTTCTTTTATATTCTCCTGACTGCAAGCCGAGTCGATAATGGATCGCAGGCTTGACTTCACCTCCGTCTCAGAATTAGAGATCGGAATATTTCCCTCAGAATTTTCGTCATTAAACACCTCAACGTTCCGACCTACATTGCGCCTACTGGTGGTGGTTGAAGGCTCGACCTGCAGAATGACAATGTATATTTTTAAAGAAATTTTATTCGATTTAAACCTTTTTTATTACTACTGTATATTGATATAAAAGCTTAGGTGGATTTAAAATCCCTTAGAAAAAATCGAGCTTAACGGCGGGATCTCCAAAAAGTTGGACAGGTTCACCCTAAGCCGTTTAATAAAATACAAATACAAATTAAGGTAAGGTGCACCTGA AGGAAACCGACTCGTTCTCTATCGTTCTCTGTTGTTTCTGAATTCTAGAGAAGATAGTCATTAAACATGCATCAAACATAAGTTATAAAAGCAAAATTATACTTGAAACCGATTTCTCTAGCCAACCAAGTTCTTTGTAAACATGAAATAAAAAATCACCCACCTGAACCACGCCTGGGAGGCCGCTTTTAACTGCTGCTCCGGTACGGATGCTGCCCACGGTGATGCTGTTAGAGATCTGCTGCCGGCGACCATGAAGTGATTGCAGTGCTAGCCGTCGCTCGTTAAGTTCCTGGTTTACTGCGTTGGCCTCTTCACCAGTGCTGTAGAGCATGCGCTGGCCCACCGTATAGCCAAAGTGGTGGTGGGCCTCCTTCAGATCCTCTAAGGGCTCTGCGCGGCACTCTAGACCTAAGCGAAAGATCTCGTCGGCCTTGCGCATGTTACCGCTCAGGTCGTAGCTGTAGGCCCAAGCAATGTAGAAATCTGCCAGCATTGTTCCGATCCCGTTGTTGTACATCTGCTGATAAAACTCGATCTTGTCCTTCTGTTTCTCCATCTAAAAAGTGACGAAGTAAAATCGGTGGTTGAGTGATCGTTTAAAATATCATCGGCTACTCACGAATTTAATGAAAAGCTTTATAAGCCGCTTGTCCTGACGGTAGCGCTCGTCGTCTTCAAATTTGGTGAGGCACTGGTGTAGGACAGTC
 

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