CR00048

CRIMIC CR00048

Gene Nep3

CG9565

Information

Donor Location Cytology FBti Stock number
pM14X:19964314..1996433219A3Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
short band no band AGTAGCCATCATTGACTTTCACATGGGC TTGAGGAACGGCGCCAGTTTGGACA

Images

Construct Design Sequences

sgRNA sequence TGAATACCCTATCTGGTGACAGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
ACACACTACCGCATACCGTG TCATGTCAGTTCGCAGAGGG AGGGTAACGATGGCTTTGAGAG AAAGTCGTGATGAGCAGCCA
 
Amplicon 5' Amplicon 3'
ACACACTACCGCATACCGTGCTCTCCCCATACACAAAGATTAACCAAAATGTGGCTTTCCGACTGCCGTTCGGTATGTCCGTGCTTCAGCTCCTGTATCCCATTCACTTTTAGTTGGGCACCTTAGTTAGACATGGAATGCTGACAAGTGGTTGAGAATGGGTTTTGTTCATGTTCATGTTTCAGACTTCGCGATTTCTTTCGAATGGATATCTAGATAGCGAGTAAAAATAGTCCAAAGATTACGAGGAACTCTTAGTACAAAATTATACATATATTTCTCTTAAGTTGTTGTGCATTACCTTTTTCTATTTCTATATTTCCATATAGTTTGGGTAATTTGCAAATTGTTCCTATGATGAATGCACATTTTCTGAAAACCCAAGGCATATCGTAAACTTGGAATGCTCTGCTGACTGCTGAAGGGTTAACACTTTTGAGTACATAGAGCACCATATGAAAATGGGCAGCAGTGGGGGATGTGAGAGAAATGTAAAGCACAAGGCGTTGAGTGAGCGCTAAAGAACGCACGGTATCTATACAATCCGAGCGGAAAAGCTTCGGTCTACTTATCAGATGCCCTAGACCAAAACATGTGATAACAGATAGCAGCAACATATTTAGATTATTAGATACACGTTGGTAGGCAATTTGATGGGTTTGCAACAATTCGATTCTCATTCCAGATGACGCGCTACAAGCAGACCGAATTCACGGAGGACGACTCGAGTTCCATCGGCGGCATTCAATTGAACGAAGCGACCGGCCATACGGGCATGCAGATACGCTACCACACCGCCCGGGTATGTAAGAAACCTGTCGGGGTAACACCGTGCATCCGTGCAATGCAATTCAGTCGGATAGCCACTTAGTTGTCGTTGGGCTTCAGCTTTATCTTTGTGGCAATGACCCAGAAACGAGTGGCAAATGGGGCCAAAGTGAGCGCTTTTCTCATTTGCTCGAAGGTTGCCAGTTGACAGTGAAAAGTCTGCTCTTATGAGTGGGAGCGCGTTGGTGTGAGCTTTTCCCTCTGCGAACTGACATGA AGGGTAACGATGGCTTTGAGAGGCGGTTCGTTGTTCAAATAAATTTAAACTTCATGTGATATTATTCCATAGATATACGAAAGCACTTGACCGTATACAACTGTATCAACAAAATGTCTAAAAGCAAGTTAAATTACTATAGAATTTAAACAGTGTGGTACAATATTTGTCTTTGGAGACTGCATTTCATGAGTCGTTGCGGCTTCTGAAAAGCCATTTATCTATATCTCAGCGATATATGGAAAAATTAGCAGGCAAGTGCCTTACGCTTACGAAATGTATCCCATGTAGATACCACCCCAATGTGTACAAGTTTGAACTCATTTGAACCGCCGTCGTCTTGTTTACAAAACACACAATTCGCTGGAAAAGATTTATGGGGCGAATCTTTTGTGCCGCCATGTGGAGGCCAGAATGATTGGTGGCTAATGCGGCGTTTTCATTTCACAGGCTACATGGAATTGGCGCTCGAGAAACAAGACGGAGAAATGGCTGCTCATCACGACTTT
 

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