MiMIC Cloning Vectors

The MiMIC constructs are available for order from the Drosophila Genomics Resource Center. DGRC barcode ordering numbers are shown below. See Venken et al. (2011) Nature Methods 8:737-743 [PDF] [Suppl] for more information about the technique.

pMiLR-attP1-2-yellow-SA-EGFP 1321 MiMIC or Mi{MIC} vector

pBS-KS-attB1-2 1322 General cloning and correction plasmid

Gene Trap
pBS-KS-attB1-2-GT-SA 1323 General gene trap plasmid
pBS-KS-attB1-2-GT-SA-mCherry-SV40 1324 mCherry gene trap plasmid
pBS-KS-attB1-2-GT-SA-GAL4-Hsp70pA 1325 GAL4 gene trap plasmid
pBS-KS-attB1-2-GT-SA-Flpo-SV40 1326 Flp gene trap plasmid
pBS-KS-attB1-2-GT-SA-QF-Hsp70 1296 QF gene trap plasmid

Phase 0
pBS-KS-attB1-2-PT-SA-SD-0 1297 General splice phase 0 protein trap plasmid
pBS-KS-attB1-2-PT-SA-SD-0-EGFP-FlAsH-StrepII-TEV-3xFlag 1298 Splice phase 0 EGFP-FlAsH-StrepII-TEV-3xFlag plasmid
pBS-KS-attB1-2-PT-SA-SD-0-mCherry 1299 Splice phase 0 mCherry plasmid
pBS-KS-attB1-2-PT-SA-SD-0-EBFP2-3xMyc 1300 Splice phase 0 EBFP2-3xMyc plasmid
pBS-KS-attB1-2-PT-SA-SD-0-TagRFP-T-3xHA 1301 Splice phase 0 TagRFP-3xHA plasmid
pBS-KS-attB1-2-PT-SA-SD-0-HRP-S 1302 Splice phase 0 HRP-S plasmid
pBS-KS-attB1-2-PT-SA-SD-0-Dendra-V5 1303 Splice phase 0 Dendra-V5 plasmid
pBS-KS-attB1-2-PT-SA-SD-0-KillerRed-V5 1304 Splice phase 0 KillerRed-V5 plasmid

Phase 1
pBS-KS-attB1-2-PT-SA-SD-1 1305 General splice phase 1 protein trap plasmid
pBS-KS-attB1-2-PT-SA-SD-1-EGFP-FlAsH-StrepII-TEV-3xFlag 1306 Splice phase 1 EGFP-FlAsH-StrepII-TEV-3xFlag plasmid
pBS-KS-attB1-2-PT-SA-SD-1-mCherry 1307 Splice phase 1 mCherry plasmid
pBS-KS-attB1-2-PT-SA-SD-1-EBFP2-3xMyc 1308 Splice phase 1 EBFP2-3xMyc plasmid
pBS-KS-attB1-2-PT-SA-SD-1-TagRFP-T-3xHA 1309 Splice phase 1 TagRFP-3xHA plasmid
pBS-KS-attB1-2-PT-SA-SD-1-HRP-S 1310 Splice phase 1 HRP-S plasmid
pBS-KS-attB1-2-PT-SA-SD-1-Dendra-V5 1311 Splice phase 1 Dendra-V5 plasmid
pBS-KS-attB1-2-PT-SA-SD-1-KillerRed-V5 1312 Splice phase 1 KillerRed-V5 plasmid

Phase 2
pBS-KS-attB1-2-PT-SA-SD-2 1313 General splice phase 2 protein trap plasmid
pBS-KS-attB1-2-PT-SA-SD-2-EGFP-FlAsH-StrepII-TEV-3xFlag 1314 Splice phase 2 EGFP-FlAsH-StrepII-TEV-3xFlag plasmid
pBS-KS-attB1-2-PT-SA-SD-2-mCherry 1315 Splice phase 2 mCherry plasmid
pBS-KS-attB1-2-PT-SA-SD-2-EBFP2-3xMyc 1316 Splice phase 2 EBFP2-3xMyc plasmid
pBS-KS-attB1-2-PT-SA-SD-2-TagRFP-T-3xHA 1317 Splice phase 2 TagRFP-3xHA plasmid
pBS-KS-attB1-2-PT-SA-SD-2-HRP-S 1318 Splice phase 2 HRP-S plasmid
pBS-KS-attB1-2-PT-SA-SD-2-Dendra-V5 1319 Splice phase 2 Dendra-V5 plasmid
pBS-KS-attB1-2-PT-SA-SD-2-KillerRed-V5 1320 Splice phase 2 KillerRed-V5 plasmid