The Gene Disruption Project

The Gene Disruption Project (GDP) is using a non-targeted transposon mutagenesis strategy to create a public collection of insertion mutants. The collection can be searched online and new mutants may be requested while they are being balanced. Balanced stocks are available from the Bloomington Drosophila Stock Center. We have currently contributed over 12,000 insertions to the public collection.
This project is a collaboration among the laboratories of Hugo Bellen (Baylor College of Medicine), Roger Hoskins (Lawrence Berkeley National Laboratory) and Allan Spradling (Carnegie Institution of Washington) and is supported by the National Institutes of Health (5R01GM067858)and the Howard Hughes Medical Institute. The flies are generated, selected, balanced and maintained by Yuchun He, Ying Fang, Zhihua Wang and Jianping Li in the Bellen lab. The inverse PCRs and sequencing are performed by Martha Evans-Holm in the Hoskins lab. Mapping information is obtained by Ben Booth in the Hoskins lab followed by manual curation by Bob Levis and Allan Spradling in the Spradling lab. The web database and strain requests are being handled by Karen Schulze in the Bellen lab. The MiMIC construct was designed by Koen Venken in the Bellen lab.
Please reference our publications, which are also found on the Downloads page, as well as the Bloomington Drosophila Stock Center, when utilizing these stocks.

  1. Nagarkar-Jaiswal S, DeLuca SZ, Lee PT, Lin WW, Pan H, Zuo Z, Lv J, Spradling AC, Bellen HJ (2015) A genetic toolkit for tagging intronic MiMIC containing genes. eLife 4:e08469. [Article]

  2. Nagarkar-Jaiswal S, Lee PT, Campbell ME, Chen K, Anguiano-Zarate S, Cantu Gutierrez M, Busby T, Lin WW, He Y, Schulze KL, Booth BW, Evans-Holm M, Venken KJ, Levis RW, Spradling AC, Hoskins RA, Bellen HJ (2015) A library of MiMICs allows tagging of genes and reversible spatial and temporal knockdown of proteins in Drosophila. eLife 4:e05338. [Article]

  3. Spradling AC, Bellen HJ, Hoskins RA (2011) Drosophila P elements preferentially transpose to replication origins. Proceedings of the National Academy of Sciences USA 108:15948-15953. [PDF] [Suppl]

  4. Venken KJT, Schulze KL, Haelterman NA, Pan H, He Y, Evans-Holm M, Carlson JW, Levis RW, Spradling AC, Hoskins RA, Bellen HJ (2011) MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes. Nature Methods 8:737-743. [PDF] [Suppl]

  5. Bellen HJ, Levis RW, He Y, Carlson JW, Evans-Holm M, Bae E, Kim J, Metaxakis A, Savakis C, Schulze KL, Hoskins RA, Spradling AC (2011) The Drosophila Gene Disruption Project: progress using transposons with distinctive site-specificities. Genetics 188:731-743. [PDF]

  6. Bellen HJ, Levis RW, Liao G, He Y, Carlson JW, Tsang G, Evans-Holm M, Hiesinger PR, Schulze KL, Rubin GM, Hoskins RA, Spradling AC (2004) The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. Genetics 167:761-781. [PDF]